Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hycF and hycG overlap by 1 nucleotides hycG and hycH overlap by 4 nucleotides hycH and hycI overlap by 8 nucleotides hycI and ascB are separated by 123 nucleotides
OKFHMN_20595: hycF - formate hydrogenlyase subunit HycF, at 4,007,954 to 4,008,496
hycF
OKFHMN_20600: hycG - formate hydrogenlyase subunit HycG, at 4,008,496 to 4,009,263
hycG
OKFHMN_20605: hycH - formate hydrogenlyase assembly protein HycH, at 4,009,260 to 4,009,670
hycH
OKFHMN_20610: hycI - hydrogenase maturation peptidase HycI, at 4,009,663 to 4,010,133
hycI
OKFHMN_20615: ascB - 6-phospho-beta-glucosidase AscB, at 4,010,257 to 4,011,681
ascB
Position (kb)
4009
4010 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 4008.364 kb on + strand, within hycF at 4008.478 kb on + strand at 4008.507 kb on - strand at 4008.741 kb on - strand, within hycG at 4008.830 kb on - strand, within hycG at 4008.830 kb on - strand, within hycG at 4008.916 kb on + strand, within hycG at 4008.916 kb on + strand, within hycG at 4008.917 kb on - strand, within hycG at 4008.917 kb on - strand, within hycG at 4009.138 kb on + strand, within hycG at 4009.138 kb on + strand, within hycG at 4009.138 kb on + strand, within hycG at 4009.144 kb on - strand, within hycG at 4009.152 kb on - strand, within hycG at 4009.254 kb on + strand at 4009.347 kb on + strand, within hycH at 4009.348 kb on - strand, within hycH at 4009.350 kb on + strand, within hycH at 4009.494 kb on + strand, within hycH at 4009.494 kb on + strand, within hycH at 4009.592 kb on + strand, within hycH at 4009.603 kb on + strand, within hycH at 4009.604 kb on - strand, within hycH at 4009.607 kb on + strand, within hycH at 4009.620 kb on - strand, within hycH at 4009.641 kb on + strand at 4009.641 kb on + strand at 4009.641 kb on + strand at 4009.676 kb on + strand at 4009.759 kb on - strand, within hycI at 4009.933 kb on - strand, within hycI at 4009.933 kb on - strand, within hycI at 4009.974 kb on + strand, within hycI at 4009.975 kb on - strand, within hycI at 4010.146 kb on + strand at 4010.146 kb on + strand at 4010.147 kb on - strand at 4010.255 kb on - strand at 4010.259 kb on - strand at 4010.298 kb on - strand at 4010.316 kb on + strand at 4010.317 kb on - strand at 4010.317 kb on - strand at 4010.376 kb on - strand at 4010.435 kb on + strand, within ascB at 4010.499 kb on - strand, within ascB at 4010.600 kb on + strand, within ascB at 4010.600 kb on + strand, within ascB at 4010.600 kb on + strand, within ascB at 4010.600 kb on + strand, within ascB at 4010.601 kb on - strand, within ascB at 4010.601 kb on - strand, within ascB at 4010.601 kb on - strand, within ascB at 4010.601 kb on - strand, within ascB at 4010.601 kb on - strand, within ascB at 4010.601 kb on - strand, within ascB at 4010.620 kb on - strand, within ascB at 4010.665 kb on + strand, within ascB at 4010.666 kb on - strand, within ascB at 4010.666 kb on - strand, within ascB at 4010.666 kb on - strand, within ascB at 4010.666 kb on - strand, within ascB
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 4,008,364 + hycF OKFHMN_20595 0.76 +0.0 4,008,478 + -0.1 4,008,507 - +0.4 4,008,741 - hycG OKFHMN_20600 0.32 +1.1 4,008,830 - hycG OKFHMN_20600 0.43 +0.3 4,008,830 - hycG OKFHMN_20600 0.43 +0.2 4,008,916 + hycG OKFHMN_20600 0.55 -0.3 4,008,916 + hycG OKFHMN_20600 0.55 +0.2 4,008,917 - hycG OKFHMN_20600 0.55 +0.1 4,008,917 - hycG OKFHMN_20600 0.55 +0.2 4,009,138 + hycG OKFHMN_20600 0.84 +0.1 4,009,138 + hycG OKFHMN_20600 0.84 -0.2 4,009,138 + hycG OKFHMN_20600 0.84 +0.7 4,009,144 - hycG OKFHMN_20600 0.84 +0.1 4,009,152 - hycG OKFHMN_20600 0.85 +1.7 4,009,254 + -0.9 4,009,347 + hycH OKFHMN_20605 0.21 +0.6 4,009,348 - hycH OKFHMN_20605 0.21 -5.2 4,009,350 + hycH OKFHMN_20605 0.22 +0.1 4,009,494 + hycH OKFHMN_20605 0.57 +0.6 4,009,494 + hycH OKFHMN_20605 0.57 +0.2 4,009,592 + hycH OKFHMN_20605 0.81 +0.4 4,009,603 + hycH OKFHMN_20605 0.83 -0.3 4,009,604 - hycH OKFHMN_20605 0.84 -0.3 4,009,607 + hycH OKFHMN_20605 0.84 -0.1 4,009,620 - hycH OKFHMN_20605 0.88 -0.4 4,009,641 + -0.3 4,009,641 + +0.7 4,009,641 + +0.2 4,009,676 + +0.7 4,009,759 - hycI OKFHMN_20610 0.20 +0.2 4,009,933 - hycI OKFHMN_20610 0.57 +1.3 4,009,933 - hycI OKFHMN_20610 0.57 -2.4 4,009,974 + hycI OKFHMN_20610 0.66 -0.3 4,009,975 - hycI OKFHMN_20610 0.66 +1.5 4,010,146 + -0.0 4,010,146 + +0.4 4,010,147 - -0.1 4,010,255 - +0.1 4,010,259 - +0.4 4,010,298 - -0.4 4,010,316 + -1.2 4,010,317 - -0.3 4,010,317 - +1.4 4,010,376 - -0.8 4,010,435 + ascB OKFHMN_20615 0.12 -1.0 4,010,499 - ascB OKFHMN_20615 0.17 -0.3 4,010,600 + ascB OKFHMN_20615 0.24 +0.7 4,010,600 + ascB OKFHMN_20615 0.24 +0.6 4,010,600 + ascB OKFHMN_20615 0.24 +0.3 4,010,600 + ascB OKFHMN_20615 0.24 +0.4 4,010,601 - ascB OKFHMN_20615 0.24 +0.1 4,010,601 - ascB OKFHMN_20615 0.24 -0.3 4,010,601 - ascB OKFHMN_20615 0.24 +0.3 4,010,601 - ascB OKFHMN_20615 0.24 +0.7 4,010,601 - ascB OKFHMN_20615 0.24 +0.2 4,010,601 - ascB OKFHMN_20615 0.24 +0.4 4,010,620 - ascB OKFHMN_20615 0.25 -1.2 4,010,665 + ascB OKFHMN_20615 0.29 -0.0 4,010,666 - ascB OKFHMN_20615 0.29 +0.3 4,010,666 - ascB OKFHMN_20615 0.29 +1.1 4,010,666 - ascB OKFHMN_20615 0.29 -0.2 4,010,666 - ascB OKFHMN_20615 0.29 +0.9
Or see this region's nucleotide sequence