Strain Fitness in Escherichia coli ECRC100 around OKFHMN_20515

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntedcC and ecdD are separated by 10 nucleotidesecdD and pphB are separated by 40 nucleotidespphB and mutS are separated by 105 nucleotides OKFHMN_20510: edcC - Phenolic acid decarboxylase, at 3,990,575 to 3,992,002 edcC OKFHMN_20515: ecdD - Protein EcdD, at 3,992,013 to 3,992,249 ecdD OKFHMN_20520: pphB - protein-serine/threonine phosphatase, at 3,992,290 to 3,992,946 pphB OKFHMN_20525: mutS - DNA mismatch repair protein MutS, at 3,993,052 to 3,995,613 mutS Position (kb) 3992 3993Strain fitness (log2 ratio) -2 -1 0 1at 3991.094 kb on - strand, within edcCat 3991.213 kb on + strand, within edcCat 3991.235 kb on + strand, within edcCat 3991.236 kb on - strand, within edcCat 3991.410 kb on - strand, within edcCat 3991.488 kb on - strand, within edcCat 3991.551 kb on - strand, within edcCat 3991.551 kb on - strand, within edcCat 3991.602 kb on - strand, within edcCat 3991.602 kb on - strand, within edcCat 3991.625 kb on - strand, within edcCat 3991.625 kb on - strand, within edcCat 3991.704 kb on - strand, within edcCat 3991.704 kb on - strand, within edcCat 3991.708 kb on - strand, within edcCat 3991.996 kb on + strandat 3991.997 kb on - strandat 3992.001 kb on - strandat 3992.087 kb on - strand, within ecdDat 3992.088 kb on + strand, within ecdDat 3992.109 kb on + strand, within ecdDat 3992.109 kb on + strand, within ecdDat 3992.109 kb on + strand, within ecdDat 3992.109 kb on + strand, within ecdDat 3992.112 kb on - strand, within ecdDat 3992.809 kb on - strand, within pphBat 3992.846 kb on - strand, within pphBat 3993.008 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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3,991,094 - edcC OKFHMN_20510 0.36 -0.2
3,991,213 + edcC OKFHMN_20510 0.45 +1.3
3,991,235 + edcC OKFHMN_20510 0.46 +0.5
3,991,236 - edcC OKFHMN_20510 0.46 +0.1
3,991,410 - edcC OKFHMN_20510 0.58 -0.2
3,991,488 - edcC OKFHMN_20510 0.64 -2.3
3,991,551 - edcC OKFHMN_20510 0.68 -0.9
3,991,551 - edcC OKFHMN_20510 0.68 -0.1
3,991,602 - edcC OKFHMN_20510 0.72 -2.2
3,991,602 - edcC OKFHMN_20510 0.72 -0.3
3,991,625 - edcC OKFHMN_20510 0.74 +0.2
3,991,625 - edcC OKFHMN_20510 0.74 -0.8
3,991,704 - edcC OKFHMN_20510 0.79 +0.4
3,991,704 - edcC OKFHMN_20510 0.79 +0.5
3,991,708 - edcC OKFHMN_20510 0.79 +0.6
3,991,996 + +0.5
3,991,997 - -0.2
3,992,001 - -0.5
3,992,087 - ecdD OKFHMN_20515 0.31 -1.4
3,992,088 + ecdD OKFHMN_20515 0.32 +0.4
3,992,109 + ecdD OKFHMN_20515 0.41 -0.7
3,992,109 + ecdD OKFHMN_20515 0.41 +0.2
3,992,109 + ecdD OKFHMN_20515 0.41 -0.7
3,992,109 + ecdD OKFHMN_20515 0.41 +1.4
3,992,112 - ecdD OKFHMN_20515 0.42 +0.2
3,992,809 - pphB OKFHMN_20520 0.79 +0.5
3,992,846 - pphB OKFHMN_20520 0.85 +0.2
3,993,008 + -0.6

Or see this region's nucleotide sequence