Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cas7e and cas5e are separated by 10 nucleotides cas5e and cas6e overlap by 19 nucleotides cas6e and cas1e overlap by 4 nucleotides cas1e and cas2e overlap by 4 nucleotides
OKFHMN_20410: cas7e - type I-E CRISPR-associated protein Cas7/Cse4/CasC, at 3,974,204 to 3,975,259
cas7e
OKFHMN_20415: cas5e - type I-E CRISPR-associated protein Cas5/CasD, at 3,975,270 to 3,976,016
cas5e
OKFHMN_20420: cas6e - type I-E CRISPR-associated protein Cas6/Cse3/CasE, at 3,975,998 to 3,976,648
cas6e
OKFHMN_20425: cas1e - type I-E CRISPR-associated endonuclease Cas1e, at 3,976,645 to 3,977,568
cas1e
OKFHMN_20430: cas2e - type I-E CRISPR-associated endoribonuclease Cas2e, at 3,977,565 to 3,977,858
cas2e
Position (kb)
3975
3976
3977 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3975.029 kb on + strand, within cas7e at 3975.120 kb on + strand, within cas7e at 3975.140 kb on - strand, within cas7e at 3975.140 kb on - strand, within cas7e at 3975.142 kb on + strand, within cas7e at 3975.143 kb on - strand, within cas7e at 3975.257 kb on + strand at 3975.430 kb on + strand, within cas5e at 3975.498 kb on + strand, within cas5e at 3975.530 kb on + strand, within cas5e at 3975.530 kb on + strand, within cas5e at 3975.688 kb on + strand, within cas5e at 3975.787 kb on + strand, within cas5e at 3975.788 kb on - strand, within cas5e at 3975.792 kb on - strand, within cas5e at 3975.798 kb on + strand, within cas5e at 3976.001 kb on + strand at 3976.001 kb on + strand at 3976.001 kb on + strand at 3976.002 kb on - strand at 3976.015 kb on - strand at 3976.020 kb on + strand at 3976.020 kb on + strand at 3976.020 kb on + strand at 3976.020 kb on + strand at 3976.021 kb on - strand at 3976.024 kb on + strand at 3976.025 kb on - strand at 3976.076 kb on + strand, within cas6e at 3976.077 kb on - strand, within cas6e at 3976.541 kb on - strand, within cas6e at 3976.655 kb on + strand at 3976.693 kb on - strand at 3976.724 kb on + strand at 3976.935 kb on + strand, within cas1e at 3976.941 kb on - strand, within cas1e at 3976.945 kb on + strand, within cas1e at 3977.002 kb on + strand, within cas1e at 3977.089 kb on + strand, within cas1e at 3977.089 kb on + strand, within cas1e at 3977.089 kb on + strand, within cas1e at 3977.096 kb on + strand, within cas1e at 3977.110 kb on + strand, within cas1e at 3977.111 kb on - strand, within cas1e at 3977.111 kb on - strand, within cas1e at 3977.206 kb on + strand, within cas1e at 3977.207 kb on - strand, within cas1e at 3977.212 kb on - strand, within cas1e at 3977.232 kb on - strand, within cas1e at 3977.262 kb on + strand, within cas1e at 3977.348 kb on + strand, within cas1e at 3977.391 kb on - strand, within cas1e at 3977.391 kb on - strand, within cas1e at 3977.435 kb on + strand, within cas1e at 3977.500 kb on - strand at 3977.608 kb on + strand, within cas2e
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 3,975,029 + cas7e OKFHMN_20410 0.78 +0.5 3,975,120 + cas7e OKFHMN_20410 0.87 -0.9 3,975,140 - cas7e OKFHMN_20410 0.89 +0.3 3,975,140 - cas7e OKFHMN_20410 0.89 +0.7 3,975,142 + cas7e OKFHMN_20410 0.89 -0.8 3,975,143 - cas7e OKFHMN_20410 0.89 +0.8 3,975,257 + -0.6 3,975,430 + cas5e OKFHMN_20415 0.21 +0.1 3,975,498 + cas5e OKFHMN_20415 0.31 +0.3 3,975,530 + cas5e OKFHMN_20415 0.35 -0.2 3,975,530 + cas5e OKFHMN_20415 0.35 +0.3 3,975,688 + cas5e OKFHMN_20415 0.56 +0.4 3,975,787 + cas5e OKFHMN_20415 0.69 -1.8 3,975,788 - cas5e OKFHMN_20415 0.69 +0.1 3,975,792 - cas5e OKFHMN_20415 0.70 +1.6 3,975,798 + cas5e OKFHMN_20415 0.71 +0.1 3,976,001 + -1.5 3,976,001 + -2.4 3,976,001 + -0.5 3,976,002 - -0.6 3,976,015 - +0.2 3,976,020 + +0.9 3,976,020 + +0.4 3,976,020 + +0.0 3,976,020 + +0.2 3,976,021 - -0.3 3,976,024 + +1.1 3,976,025 - -0.7 3,976,076 + cas6e OKFHMN_20420 0.12 -1.9 3,976,077 - cas6e OKFHMN_20420 0.12 +0.3 3,976,541 - cas6e OKFHMN_20420 0.83 +0.2 3,976,655 + -0.6 3,976,693 - +0.3 3,976,724 + +0.5 3,976,935 + cas1e OKFHMN_20425 0.31 +0.3 3,976,941 - cas1e OKFHMN_20425 0.32 +0.1 3,976,945 + cas1e OKFHMN_20425 0.32 +0.8 3,977,002 + cas1e OKFHMN_20425 0.39 -0.1 3,977,089 + cas1e OKFHMN_20425 0.48 +0.2 3,977,089 + cas1e OKFHMN_20425 0.48 -0.5 3,977,089 + cas1e OKFHMN_20425 0.48 -1.8 3,977,096 + cas1e OKFHMN_20425 0.49 -0.4 3,977,110 + cas1e OKFHMN_20425 0.50 -0.2 3,977,111 - cas1e OKFHMN_20425 0.50 +0.3 3,977,111 - cas1e OKFHMN_20425 0.50 +0.4 3,977,206 + cas1e OKFHMN_20425 0.61 -0.0 3,977,207 - cas1e OKFHMN_20425 0.61 -0.5 3,977,212 - cas1e OKFHMN_20425 0.61 +0.3 3,977,232 - cas1e OKFHMN_20425 0.64 -1.2 3,977,262 + cas1e OKFHMN_20425 0.67 -1.7 3,977,348 + cas1e OKFHMN_20425 0.76 -0.2 3,977,391 - cas1e OKFHMN_20425 0.81 +0.2 3,977,391 - cas1e OKFHMN_20425 0.81 -3.3 3,977,435 + cas1e OKFHMN_20425 0.85 -0.0 3,977,500 - -0.8 3,977,608 + cas2e OKFHMN_20430 0.15 +0.8
Or see this region's nucleotide sequence