Strain Fitness in Escherichia coli ECRC100 around OKFHMN_20390

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcysH and hokG are separated by 263 nucleotideshokG and cas3 are separated by 191 nucleotides OKFHMN_20380: cysH - phosphoadenosine phosphosulfate reductase, at 3,967,958 to 3,968,692 cysH OKFHMN_20390: hokG - Putative protein HokG, at 3,968,956 to 3,969,108 hokG OKFHMN_20395: cas3 - CRISPR-associated helicase/endonuclease Cas3, at 3,969,300 to 3,971,999 cas3 Position (kb) 3968 3969 3970Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 3967.958 kb on - strandat 3968.029 kb on + strandat 3968.100 kb on - strand, within cysHat 3968.236 kb on - strand, within cysHat 3968.268 kb on - strand, within cysHat 3968.349 kb on - strand, within cysHat 3968.526 kb on + strand, within cysHat 3968.528 kb on + strand, within cysHat 3968.558 kb on + strand, within cysHat 3968.605 kb on + strand, within cysHat 3968.803 kb on + strandat 3968.804 kb on - strandat 3968.804 kb on - strandat 3968.880 kb on + strandat 3968.928 kb on + strandat 3968.988 kb on - strand, within hokGat 3968.988 kb on - strand, within hokGat 3968.988 kb on - strand, within hokGat 3968.991 kb on - strand, within hokGat 3969.000 kb on + strand, within hokGat 3969.000 kb on + strand, within hokGat 3969.000 kb on + strand, within hokGat 3969.038 kb on + strand, within hokGat 3969.338 kb on + strandat 3969.594 kb on + strand, within cas3at 3969.595 kb on - strand, within cas3at 3969.743 kb on + strand, within cas3at 3969.744 kb on - strand, within cas3at 3969.754 kb on + strand, within cas3at 3969.755 kb on - strand, within cas3at 3969.807 kb on - strand, within cas3at 3969.955 kb on - strand, within cas3at 3969.956 kb on + strand, within cas3at 3969.957 kb on - strand, within cas3at 3970.061 kb on - strand, within cas3

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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3,967,958 - -3.6
3,968,029 + -2.4
3,968,100 - cysH OKFHMN_20380 0.19 -4.3
3,968,236 - cysH OKFHMN_20380 0.38 -1.3
3,968,268 - cysH OKFHMN_20380 0.42 -2.0
3,968,349 - cysH OKFHMN_20380 0.53 +1.1
3,968,526 + cysH OKFHMN_20380 0.77 -0.9
3,968,528 + cysH OKFHMN_20380 0.78 -3.1
3,968,558 + cysH OKFHMN_20380 0.82 -4.2
3,968,605 + cysH OKFHMN_20380 0.88 -1.5
3,968,803 + -0.9
3,968,804 - +0.7
3,968,804 - -0.4
3,968,880 + +0.3
3,968,928 + -0.5
3,968,988 - hokG OKFHMN_20390 0.21 -0.6
3,968,988 - hokG OKFHMN_20390 0.21 +1.1
3,968,988 - hokG OKFHMN_20390 0.21 +0.1
3,968,991 - hokG OKFHMN_20390 0.23 -0.1
3,969,000 + hokG OKFHMN_20390 0.29 -0.1
3,969,000 + hokG OKFHMN_20390 0.29 -0.1
3,969,000 + hokG OKFHMN_20390 0.29 -2.8
3,969,038 + hokG OKFHMN_20390 0.54 +1.7
3,969,338 + +1.0
3,969,594 + cas3 OKFHMN_20395 0.11 -1.0
3,969,595 - cas3 OKFHMN_20395 0.11 +2.3
3,969,743 + cas3 OKFHMN_20395 0.16 +0.5
3,969,744 - cas3 OKFHMN_20395 0.16 -0.1
3,969,754 + cas3 OKFHMN_20395 0.17 +0.5
3,969,755 - cas3 OKFHMN_20395 0.17 -0.7
3,969,807 - cas3 OKFHMN_20395 0.19 -0.5
3,969,955 - cas3 OKFHMN_20395 0.24 -0.2
3,969,956 + cas3 OKFHMN_20395 0.24 +0.2
3,969,957 - cas3 OKFHMN_20395 0.24 -0.9
3,970,061 - cas3 OKFHMN_20395 0.28 -0.9

Or see this region's nucleotide sequence