Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gpsA and cysE are separated by 79 nucleotides cysE and trmL are separated by 52 nucleotides trmL and lldD are separated by 185 nucleotides
OKFHMN_15755: gpsA - NAD(P)H-dependent glycerol-3-phosphate dehydrogenase, at 3,044,585 to 3,045,604
gpsA
OKFHMN_15760: cysE - serine O-acetyltransferase, at 3,045,684 to 3,046,505
cysE
OKFHMN_15765: trmL - tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine (34)-2'-O)-methyltransferase TrmL, at 3,046,558 to 3,047,031
trmL
OKFHMN_15770: lldD - quinone-dependent L-lactate dehydrogenase, at 3,047,217 to 3,048,407
lldD
Position (kb)
3045
3046
3047 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3045.661 kb on + strand at 3045.662 kb on - strand at 3045.662 kb on - strand at 3046.409 kb on + strand, within cysE at 3046.418 kb on + strand, within cysE at 3046.433 kb on + strand at 3046.434 kb on - strand at 3046.472 kb on + strand at 3046.472 kb on + strand at 3046.478 kb on + strand at 3046.503 kb on + strand at 3046.503 kb on + strand at 3046.503 kb on + strand at 3046.503 kb on + strand at 3046.504 kb on - strand at 3046.504 kb on - strand at 3046.504 kb on - strand at 3046.533 kb on + strand at 3046.533 kb on + strand at 3046.534 kb on - strand at 3046.534 kb on - strand at 3046.534 kb on - strand at 3046.556 kb on - strand at 3046.559 kb on + strand at 3046.559 kb on + strand at 3046.607 kb on + strand, within trmL at 3046.607 kb on + strand, within trmL at 3046.607 kb on + strand, within trmL at 3046.765 kb on + strand, within trmL at 3046.786 kb on - strand, within trmL at 3046.942 kb on - strand, within trmL at 3046.959 kb on + strand, within trmL at 3046.960 kb on - strand, within trmL at 3046.963 kb on - strand, within trmL at 3046.980 kb on + strand, within trmL at 3046.980 kb on + strand, within trmL at 3046.981 kb on - strand, within trmL at 3046.989 kb on + strand at 3046.990 kb on - strand at 3047.013 kb on - strand at 3047.017 kb on + strand at 3047.017 kb on + strand at 3047.018 kb on - strand at 3047.026 kb on + strand at 3047.026 kb on + strand at 3047.031 kb on + strand at 3047.059 kb on - strand at 3047.152 kb on + strand at 3047.152 kb on + strand at 3047.153 kb on - strand at 3047.156 kb on + strand at 3047.156 kb on + strand at 3047.156 kb on + strand at 3047.156 kb on + strand at 3047.157 kb on - strand at 3047.157 kb on - strand at 3047.377 kb on - strand, within lldD at 3047.408 kb on - strand, within lldD at 3047.457 kb on - strand, within lldD
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 3,045,661 + +0.4 3,045,662 - +2.1 3,045,662 - -2.7 3,046,409 + cysE OKFHMN_15760 0.88 -3.0 3,046,418 + cysE OKFHMN_15760 0.89 -0.6 3,046,433 + -0.2 3,046,434 - +1.9 3,046,472 + -0.4 3,046,472 + +0.1 3,046,478 + -0.3 3,046,503 + -0.2 3,046,503 + +0.5 3,046,503 + -0.1 3,046,503 + -0.8 3,046,504 - +1.5 3,046,504 - +0.7 3,046,504 - +0.7 3,046,533 + +1.2 3,046,533 + -1.9 3,046,534 - +0.1 3,046,534 - +0.3 3,046,534 - +0.5 3,046,556 - -0.4 3,046,559 + +0.6 3,046,559 + -0.2 3,046,607 + trmL OKFHMN_15765 0.10 -0.1 3,046,607 + trmL OKFHMN_15765 0.10 +0.8 3,046,607 + trmL OKFHMN_15765 0.10 -0.2 3,046,765 + trmL OKFHMN_15765 0.44 -0.3 3,046,786 - trmL OKFHMN_15765 0.48 -0.2 3,046,942 - trmL OKFHMN_15765 0.81 -2.1 3,046,959 + trmL OKFHMN_15765 0.85 +1.5 3,046,960 - trmL OKFHMN_15765 0.85 -0.0 3,046,963 - trmL OKFHMN_15765 0.85 -0.2 3,046,980 + trmL OKFHMN_15765 0.89 +0.6 3,046,980 + trmL OKFHMN_15765 0.89 +0.1 3,046,981 - trmL OKFHMN_15765 0.89 -0.6 3,046,989 + +0.3 3,046,990 - +3.4 3,047,013 - +0.6 3,047,017 + +0.3 3,047,017 + +0.6 3,047,018 - +0.0 3,047,026 + +0.2 3,047,026 + -0.4 3,047,031 + +0.2 3,047,059 - +0.1 3,047,152 + -0.8 3,047,152 + +0.3 3,047,153 - -2.2 3,047,156 + -2.7 3,047,156 + +0.2 3,047,156 + +0.1 3,047,156 + -1.3 3,047,157 - -0.0 3,047,157 - -0.5 3,047,377 - lldD OKFHMN_15770 0.13 -0.6 3,047,408 - lldD OKFHMN_15770 0.16 -0.2 3,047,457 - lldD OKFHMN_15770 0.20 -2.0
Or see this region's nucleotide sequence