Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fabG and manX are separated by 9 nucleotides manX and agaB overlap by 1 nucleotides agaB and manY are separated by 65 nucleotides manY and sorM are separated by 10 nucleotides sorM and sorE are separated by 51 nucleotides
OKFHMN_13200: fabG - sorbitol-6-phosphate 2-dehydrogenase, at 2,531,421 to 2,532,227
fabG
OKFHMN_13205: manX - PTS sorbose transporter subunit IIA, at 2,532,237 to 2,532,644
manX
OKFHMN_13210: agaB - PTS fructose transporter subunit IIB, at 2,532,644 to 2,533,138
agaB
OKFHMN_13215: manY - PTS mannose/fructose/sorbose transporter subunit IIC, at 2,533,204 to 2,534,001
manY
OKFHMN_13220: sorM - PTS system sorbose-specific EIID component, at 2,534,012 to 2,534,836
sorM
OKFHMN_13225: sorE - L-sorbose 1-phosphate reductase, at 2,534,888 to 2,536,117
sorE
Position (kb)
2533
2534
2535 Strain fitness (log2 ratio)
-2
-1
0
1 at 2532.237 kb on - strand at 2532.272 kb on - strand at 2532.307 kb on - strand, within manX at 2532.386 kb on - strand, within manX at 2532.652 kb on + strand at 2532.733 kb on + strand, within agaB at 2532.736 kb on + strand, within agaB at 2532.781 kb on - strand, within agaB at 2532.781 kb on - strand, within agaB at 2533.158 kb on + strand at 2533.159 kb on - strand at 2533.159 kb on - strand at 2533.439 kb on + strand, within manY at 2533.710 kb on + strand, within manY at 2533.796 kb on + strand, within manY at 2533.797 kb on - strand, within manY at 2533.797 kb on - strand, within manY at 2533.797 kb on - strand, within manY at 2533.800 kb on + strand, within manY at 2533.800 kb on + strand, within manY at 2533.801 kb on - strand, within manY at 2533.801 kb on - strand, within manY at 2533.916 kb on - strand, within manY at 2533.925 kb on + strand at 2534.098 kb on + strand, within sorM at 2534.099 kb on - strand, within sorM at 2534.120 kb on + strand, within sorM at 2534.158 kb on - strand, within sorM at 2534.220 kb on + strand, within sorM at 2534.221 kb on - strand, within sorM at 2534.221 kb on - strand, within sorM at 2534.444 kb on - strand, within sorM at 2534.444 kb on - strand, within sorM at 2534.477 kb on + strand, within sorM at 2534.477 kb on + strand, within sorM at 2534.622 kb on + strand, within sorM at 2534.622 kb on + strand, within sorM at 2534.622 kb on + strand, within sorM at 2534.773 kb on - strand at 2534.799 kb on + strand at 2534.888 kb on - strand at 2534.913 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 2,532,237 - +0.6 2,532,272 - +0.3 2,532,307 - manX OKFHMN_13205 0.17 +0.4 2,532,386 - manX OKFHMN_13205 0.37 -0.4 2,532,652 + +0.1 2,532,733 + agaB OKFHMN_13210 0.18 -0.0 2,532,736 + agaB OKFHMN_13210 0.19 +0.1 2,532,781 - agaB OKFHMN_13210 0.28 -0.1 2,532,781 - agaB OKFHMN_13210 0.28 +0.3 2,533,158 + +0.3 2,533,159 - -0.0 2,533,159 - -0.9 2,533,439 + manY OKFHMN_13215 0.29 +0.9 2,533,710 + manY OKFHMN_13215 0.63 -0.2 2,533,796 + manY OKFHMN_13215 0.74 -0.4 2,533,797 - manY OKFHMN_13215 0.74 -1.9 2,533,797 - manY OKFHMN_13215 0.74 -0.4 2,533,797 - manY OKFHMN_13215 0.74 -1.4 2,533,800 + manY OKFHMN_13215 0.75 +0.1 2,533,800 + manY OKFHMN_13215 0.75 +0.5 2,533,801 - manY OKFHMN_13215 0.75 +0.3 2,533,801 - manY OKFHMN_13215 0.75 +0.2 2,533,916 - manY OKFHMN_13215 0.89 +0.2 2,533,925 + +1.1 2,534,098 + sorM OKFHMN_13220 0.10 -0.7 2,534,099 - sorM OKFHMN_13220 0.11 +0.7 2,534,120 + sorM OKFHMN_13220 0.13 -0.2 2,534,158 - sorM OKFHMN_13220 0.18 +1.4 2,534,220 + sorM OKFHMN_13220 0.25 -0.1 2,534,221 - sorM OKFHMN_13220 0.25 -0.5 2,534,221 - sorM OKFHMN_13220 0.25 +0.4 2,534,444 - sorM OKFHMN_13220 0.52 +1.0 2,534,444 - sorM OKFHMN_13220 0.52 +0.6 2,534,477 + sorM OKFHMN_13220 0.56 -0.0 2,534,477 + sorM OKFHMN_13220 0.56 -0.4 2,534,622 + sorM OKFHMN_13220 0.74 -0.5 2,534,622 + sorM OKFHMN_13220 0.74 +0.1 2,534,622 + sorM OKFHMN_13220 0.74 +0.0 2,534,773 - +0.3 2,534,799 + -1.1 2,534,888 - +0.2 2,534,913 - +0.1
Or see this region's nucleotide sequence