Strain Fitness in Escherichia coli ECRC100 around OKFHMN_08415

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntthiI and xseB are separated by 205 nucleotidesxseB and ispA overlap by 1 nucleotidesispA and dxs are separated by 24 nucleotides OKFHMN_08405: thiI - tRNA sulfurtransferase, at 1,563,953 to 1,565,401 thiI OKFHMN_08410: xseB - exodeoxyribonuclease VII small subunit, at 1,565,607 to 1,565,849 xseB OKFHMN_08415: ispA - (2E,6E)-farnesyl diphosphate synthase, at 1,565,849 to 1,566,748 ispA OKFHMN_08420: dxs - 1-deoxy-D-xylulose-5-phosphate synthase, at 1,566,773 to 1,568,635 dxs Position (kb) 1565 1566 1567Strain fitness (log2 ratio) -3 -2 -1 0 1at 1564.916 kb on - strand, within thiIat 1565.045 kb on - strand, within thiIat 1565.176 kb on + strand, within thiIat 1565.177 kb on - strand, within thiIat 1565.177 kb on - strand, within thiIat 1565.194 kb on + strand, within thiIat 1565.258 kb on - strandat 1565.293 kb on - strandat 1565.393 kb on - strandat 1565.439 kb on + strandat 1565.439 kb on + strandat 1565.440 kb on - strandat 1565.491 kb on + strandat 1565.492 kb on - strandat 1565.492 kb on - strandat 1565.493 kb on + strandat 1565.499 kb on + strandat 1565.527 kb on + strandat 1565.528 kb on - strandat 1565.541 kb on - strandat 1565.541 kb on - strandat 1565.585 kb on + strandat 1565.606 kb on + strandat 1565.671 kb on + strand, within xseBat 1565.785 kb on + strand, within xseB

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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1,564,916 - thiI OKFHMN_08405 0.66 +1.4
1,565,045 - thiI OKFHMN_08405 0.75 +1.2
1,565,176 + thiI OKFHMN_08405 0.84 -2.2
1,565,177 - thiI OKFHMN_08405 0.84 -0.5
1,565,177 - thiI OKFHMN_08405 0.84 +0.1
1,565,194 + thiI OKFHMN_08405 0.86 +0.2
1,565,258 - -3.5
1,565,293 - -0.3
1,565,393 - -1.1
1,565,439 + -0.2
1,565,439 + +0.3
1,565,440 - -2.0
1,565,491 + +0.4
1,565,492 - -0.3
1,565,492 - -0.2
1,565,493 + -1.3
1,565,499 + -1.2
1,565,527 + -0.3
1,565,528 - +0.3
1,565,541 - +0.2
1,565,541 - +0.4
1,565,585 + +0.2
1,565,606 + -0.7
1,565,671 + xseB OKFHMN_08410 0.26 +0.2
1,565,785 + xseB OKFHMN_08410 0.73 -0.6

Or see this region's nucleotide sequence