Strain Fitness in Escherichia coli ECRC100 around OKFHMN_07820

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOKFHMN_07805 and ybcJ are separated by 1 nucleotidesybcJ and ybcI are separated by 107 nucleotidesybcI and cysS are separated by 35 nucleotidescysS and ppiB are separated by 173 nucleotidesppiB and lpxH are separated by 2 nucleotides OKFHMN_07805: OKFHMN_07805 - Bifunctional protein FolD, at 1,423,118 to 1,423,984 _07805 OKFHMN_07810: ybcJ - ribosome-associated protein YbcJ, at 1,423,986 to 1,424,198 ybcJ OKFHMN_07815: ybcI - Inner membrane protein YbcI, at 1,424,306 to 1,424,827 ybcI OKFHMN_07820: cysS - cysteine--tRNA ligase, at 1,424,863 to 1,426,248 cysS OKFHMN_07825: ppiB - peptidylprolyl isomerase B, at 1,426,422 to 1,426,916 ppiB OKFHMN_07830: lpxH - UDP-2,3-diacylglucosamine diphosphatase, at 1,426,919 to 1,427,641 lpxH Position (kb) 1424 1425 1426 1427Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1423.961 kb on + strandat 1423.982 kb on + strandat 1423.983 kb on - strandat 1424.003 kb on - strandat 1424.006 kb on + strandat 1424.388 kb on + strand, within ybcIat 1424.456 kb on + strand, within ybcIat 1424.523 kb on + strand, within ybcIat 1424.524 kb on - strand, within ybcIat 1424.856 kb on - strandat 1426.290 kb on - strandat 1426.290 kb on - strandat 1426.290 kb on - strandat 1426.295 kb on - strandat 1426.322 kb on - strandat 1426.322 kb on - strandat 1426.355 kb on + strandat 1426.355 kb on + strandat 1426.368 kb on + strandat 1426.368 kb on + strandat 1426.370 kb on + strandat 1426.370 kb on + strandat 1426.370 kb on + strandat 1426.370 kb on + strandat 1426.370 kb on + strandat 1426.372 kb on + strandat 1426.372 kb on + strandat 1426.372 kb on + strandat 1426.372 kb on + strandat 1426.372 kb on + strandat 1426.372 kb on + strandat 1426.372 kb on + strandat 1426.372 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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1,423,961 + +1.4
1,423,982 + +0.3
1,423,983 - +0.2
1,424,003 - -1.9
1,424,006 + +0.3
1,424,388 + ybcI OKFHMN_07815 0.16 -0.6
1,424,456 + ybcI OKFHMN_07815 0.29 +1.1
1,424,523 + ybcI OKFHMN_07815 0.42 +0.0
1,424,524 - ybcI OKFHMN_07815 0.42 +0.8
1,424,856 - -0.1
1,426,290 - +0.3
1,426,290 - +0.5
1,426,290 - +2.1
1,426,295 - -0.5
1,426,322 - +0.5
1,426,322 - +0.0
1,426,355 + +0.1
1,426,355 + +1.5
1,426,368 + -1.8
1,426,368 + -0.8
1,426,370 + +0.3
1,426,370 + -0.1
1,426,370 + -1.0
1,426,370 + +0.2
1,426,370 + +1.1
1,426,372 + -0.1
1,426,372 + +0.3
1,426,372 + -0.2
1,426,372 + -0.6
1,426,372 + +0.3
1,426,372 + +0.4
1,426,372 + -0.1
1,426,372 + +3.4

Or see this region's nucleotide sequence