Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nadD and cobC are separated by 23 nucleotides cobC and rsfS are separated by 259 nucleotides rsfS and rlmH are separated by 3 nucleotides rlmH and mrdA are separated by 30 nucleotides
OKFHMN_07345: nadD - nicotinate-nucleotide adenylyltransferase, at 1,321,373 to 1,322,014
nadD
OKFHMN_07350: cobC - adenosylcobalamin/alpha-ribazole phosphatase, at 1,322,038 to 1,322,649
cobC
OKFHMN_07355: rsfS - ribosome silencing factor, at 1,322,909 to 1,323,226
rsfS
OKFHMN_07360: rlmH - 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH, at 1,323,230 to 1,323,697
rlmH
OKFHMN_07365: mrdA - peptidoglycan DD-transpeptidase MrdA, at 1,323,728 to 1,325,629
mrdA
Position (kb)
1322
1323
1324 Strain fitness (log2 ratio)
-1
0
1 at 1322.006 kb on + strand at 1322.006 kb on + strand at 1322.015 kb on + strand at 1322.016 kb on - strand at 1322.162 kb on + strand, within cobC at 1322.190 kb on - strand, within cobC at 1322.344 kb on + strand, within cobC at 1322.492 kb on + strand, within cobC at 1322.496 kb on + strand, within cobC at 1322.497 kb on - strand, within cobC at 1322.504 kb on + strand, within cobC at 1322.538 kb on + strand, within cobC at 1322.583 kb on + strand, within cobC at 1322.585 kb on + strand, within cobC at 1322.670 kb on + strand at 1322.708 kb on + strand at 1322.802 kb on + strand at 1322.884 kb on + strand at 1322.884 kb on + strand at 1322.884 kb on + strand at 1323.052 kb on + strand, within rsfS at 1323.052 kb on + strand, within rsfS at 1323.052 kb on + strand, within rsfS at 1323.052 kb on + strand, within rsfS at 1323.114 kb on + strand, within rsfS at 1323.200 kb on + strand at 1323.200 kb on + strand at 1323.296 kb on + strand, within rlmH
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,322,006 + +1.0 1,322,006 + +0.1 1,322,015 + +0.1 1,322,016 - +0.4 1,322,162 + cobC OKFHMN_07350 0.20 -0.4 1,322,190 - cobC OKFHMN_07350 0.25 -1.0 1,322,344 + cobC OKFHMN_07350 0.50 +0.2 1,322,492 + cobC OKFHMN_07350 0.74 -0.1 1,322,496 + cobC OKFHMN_07350 0.75 +0.1 1,322,497 - cobC OKFHMN_07350 0.75 -0.4 1,322,504 + cobC OKFHMN_07350 0.76 -1.4 1,322,538 + cobC OKFHMN_07350 0.82 +0.6 1,322,583 + cobC OKFHMN_07350 0.89 -0.8 1,322,585 + cobC OKFHMN_07350 0.89 -0.1 1,322,670 + -0.2 1,322,708 + +0.6 1,322,802 + +0.1 1,322,884 + +0.2 1,322,884 + -0.7 1,322,884 + +0.7 1,323,052 + rsfS OKFHMN_07355 0.45 -1.6 1,323,052 + rsfS OKFHMN_07355 0.45 -0.3 1,323,052 + rsfS OKFHMN_07355 0.45 +0.1 1,323,052 + rsfS OKFHMN_07355 0.45 +0.7 1,323,114 + rsfS OKFHMN_07355 0.64 +0.2 1,323,200 + +0.8 1,323,200 + -0.2 1,323,296 + rlmH OKFHMN_07360 0.14 +0.5
Or see this region's nucleotide sequence