Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nagD and asnB are separated by 256 nucleotides asnB and Met_trna are separated by 379 nucleotides Met_trna and Leu_trna are separated by 10 nucleotides Leu_trna and Gln_trna are separated by 23 nucleotides Gln_trna and Gln_trna are separated by 34 nucleotides Gln_trna and Met_trna are separated by 15 nucleotides Met_trna and Gln_trna are separated by 48 nucleotides
OKFHMN_07175: nagD - ribonucleotide monophosphatase NagD, at 1,292,954 to 1,293,706
nagD
OKFHMN_07180: asnB - asparagine synthase B, at 1,293,963 to 1,295,627
asnB
OKFHMN_07185: Met_trna - tRNA-Met(cat), at 1,296,007 to 1,296,083
_trna
OKFHMN_07190: Leu_trna - tRNA-Leu(tag), at 1,296,094 to 1,296,178
_trna
OKFHMN_07195: Gln_trna - tRNA-Gln(ttg), at 1,296,202 to 1,296,276
_trna
OKFHMN_07200: Gln_trna - tRNA-Gln(ttg), at 1,296,311 to 1,296,385
_trna
OKFHMN_07205: Met_trna - tRNA-Met(cat), at 1,296,401 to 1,296,477
_trna
OKFHMN_07210: Gln_trna - tRNA-Gln(ctg), at 1,296,526 to 1,296,600
_trna
Position (kb) 1293
1294
1295
1296 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1292.970 kb on + strand at 1293.158 kb on + strand, within nagD at 1293.215 kb on + strand, within nagD at 1293.216 kb on - strand, within nagD at 1293.252 kb on - strand, within nagD at 1293.337 kb on + strand, within nagD at 1293.470 kb on + strand, within nagD at 1293.470 kb on + strand, within nagD at 1293.471 kb on - strand, within nagD at 1293.471 kb on - strand, within nagD at 1293.471 kb on - strand, within nagD at 1293.510 kb on + strand, within nagD at 1293.649 kb on + strand at 1293.649 kb on + strand at 1293.649 kb on + strand at 1293.649 kb on + strand at 1293.650 kb on - strand at 1293.650 kb on - strand at 1293.941 kb on + strand at 1293.942 kb on - strand at 1293.962 kb on + strand at 1294.078 kb on - strand at 1294.153 kb on - strand, within asnB at 1294.175 kb on - strand, within asnB at 1294.175 kb on - strand, within asnB at 1294.282 kb on - strand, within asnB at 1294.363 kb on - strand, within asnB at 1294.401 kb on + strand, within asnB at 1294.401 kb on + strand, within asnB at 1294.401 kb on + strand, within asnB at 1294.402 kb on - strand, within asnB at 1294.402 kb on - strand, within asnB at 1294.402 kb on - strand, within asnB at 1294.402 kb on - strand, within asnB at 1294.402 kb on - strand, within asnB at 1294.402 kb on - strand, within asnB at 1294.402 kb on - strand, within asnB at 1294.404 kb on - strand, within asnB at 1294.431 kb on + strand, within asnB at 1294.432 kb on - strand, within asnB at 1294.527 kb on - strand, within asnB at 1294.533 kb on + strand, within asnB at 1294.533 kb on + strand, within asnB at 1294.534 kb on - strand, within asnB at 1294.537 kb on - strand, within asnB at 1294.622 kb on + strand, within asnB at 1294.765 kb on + strand, within asnB at 1294.766 kb on - strand, within asnB at 1294.781 kb on - strand, within asnB at 1294.867 kb on + strand, within asnB at 1294.954 kb on - strand, within asnB at 1295.040 kb on + strand, within asnB at 1295.041 kb on - strand, within asnB at 1295.091 kb on - strand, within asnB at 1295.108 kb on + strand, within asnB at 1295.109 kb on - strand, within asnB at 1295.109 kb on - strand, within asnB at 1295.109 kb on - strand, within asnB at 1295.112 kb on + strand, within asnB at 1295.113 kb on - strand, within asnB at 1295.113 kb on - strand, within asnB at 1295.113 kb on - strand, within asnB at 1295.113 kb on - strand, within asnB at 1295.280 kb on + strand, within asnB at 1295.280 kb on + strand, within asnB at 1295.448 kb on + strand, within asnB at 1295.449 kb on - strand, within asnB at 1295.449 kb on - strand, within asnB at 1295.621 kb on + strand at 1295.621 kb on + strand at 1295.622 kb on - strand at 1295.671 kb on - strand at 1295.694 kb on + strand at 1295.771 kb on + strand at 1295.785 kb on + strand at 1295.871 kb on + strand at 1295.896 kb on - strand at 1296.604 kb on + strand at 1296.624 kb on + strand at 1296.624 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,292,970 + +0.2 1,293,158 + nagD OKFHMN_07175 0.27 +0.2 1,293,215 + nagD OKFHMN_07175 0.35 -0.1 1,293,216 - nagD OKFHMN_07175 0.35 +0.2 1,293,252 - nagD OKFHMN_07175 0.40 +0.1 1,293,337 + nagD OKFHMN_07175 0.51 +0.7 1,293,470 + nagD OKFHMN_07175 0.69 +1.5 1,293,470 + nagD OKFHMN_07175 0.69 +0.2 1,293,471 - nagD OKFHMN_07175 0.69 +0.8 1,293,471 - nagD OKFHMN_07175 0.69 -3.0 1,293,471 - nagD OKFHMN_07175 0.69 +0.4 1,293,510 + nagD OKFHMN_07175 0.74 +0.2 1,293,649 + -0.9 1,293,649 + +0.3 1,293,649 + +0.1 1,293,649 + +0.2 1,293,650 - -0.4 1,293,650 - -1.5 1,293,941 + -0.0 1,293,942 - +0.5 1,293,962 + +0.2 1,294,078 - +0.4 1,294,153 - asnB OKFHMN_07180 0.11 +0.5 1,294,175 - asnB OKFHMN_07180 0.13 +0.8 1,294,175 - asnB OKFHMN_07180 0.13 +0.0 1,294,282 - asnB OKFHMN_07180 0.19 +0.0 1,294,363 - asnB OKFHMN_07180 0.24 +0.6 1,294,401 + asnB OKFHMN_07180 0.26 -1.6 1,294,401 + asnB OKFHMN_07180 0.26 +0.5 1,294,401 + asnB OKFHMN_07180 0.26 +0.4 1,294,402 - asnB OKFHMN_07180 0.26 -0.3 1,294,402 - asnB OKFHMN_07180 0.26 +0.4 1,294,402 - asnB OKFHMN_07180 0.26 -0.3 1,294,402 - asnB OKFHMN_07180 0.26 +0.6 1,294,402 - asnB OKFHMN_07180 0.26 -0.8 1,294,402 - asnB OKFHMN_07180 0.26 +0.2 1,294,402 - asnB OKFHMN_07180 0.26 -1.1 1,294,404 - asnB OKFHMN_07180 0.26 +1.3 1,294,431 + asnB OKFHMN_07180 0.28 +0.2 1,294,432 - asnB OKFHMN_07180 0.28 +0.5 1,294,527 - asnB OKFHMN_07180 0.34 -1.6 1,294,533 + asnB OKFHMN_07180 0.34 -0.9 1,294,533 + asnB OKFHMN_07180 0.34 -0.4 1,294,534 - asnB OKFHMN_07180 0.34 +0.6 1,294,537 - asnB OKFHMN_07180 0.34 +1.7 1,294,622 + asnB OKFHMN_07180 0.40 +0.1 1,294,765 + asnB OKFHMN_07180 0.48 +0.8 1,294,766 - asnB OKFHMN_07180 0.48 -1.2 1,294,781 - asnB OKFHMN_07180 0.49 +0.2 1,294,867 + asnB OKFHMN_07180 0.54 -0.3 1,294,954 - asnB OKFHMN_07180 0.60 -0.2 1,295,040 + asnB OKFHMN_07180 0.65 -2.6 1,295,041 - asnB OKFHMN_07180 0.65 +0.1 1,295,091 - asnB OKFHMN_07180 0.68 -2.2 1,295,108 + asnB OKFHMN_07180 0.69 -0.3 1,295,109 - asnB OKFHMN_07180 0.69 +0.4 1,295,109 - asnB OKFHMN_07180 0.69 -0.1 1,295,109 - asnB OKFHMN_07180 0.69 +0.5 1,295,112 + asnB OKFHMN_07180 0.69 -0.6 1,295,113 - asnB OKFHMN_07180 0.69 +0.6 1,295,113 - asnB OKFHMN_07180 0.69 +0.0 1,295,113 - asnB OKFHMN_07180 0.69 +1.2 1,295,113 - asnB OKFHMN_07180 0.69 +0.7 1,295,280 + asnB OKFHMN_07180 0.79 -0.3 1,295,280 + asnB OKFHMN_07180 0.79 +0.5 1,295,448 + asnB OKFHMN_07180 0.89 -0.3 1,295,449 - asnB OKFHMN_07180 0.89 -0.4 1,295,449 - asnB OKFHMN_07180 0.89 -0.3 1,295,621 + +0.2 1,295,621 + -1.0 1,295,622 - -0.1 1,295,671 - +0.8 1,295,694 + -0.8 1,295,771 + +0.1 1,295,785 + -0.0 1,295,871 + -0.7 1,295,896 - -0.4 1,296,604 + -0.9 1,296,624 + -0.0 1,296,624 + +0.1
Or see this region's nucleotide sequence