Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybiY and OKFHMN_06140 are separated by 5 nucleotides OKFHMN_06140 and pflD are separated by 77 nucleotides pflD and ybiV are separated by 145 nucleotides
OKFHMN_06135: ybiY - Putative pyruvate formate-lyase 3-activating enzyme, at 1,085,532 to 1,086,431
ybiY
OKFHMN_06140: OKFHMN_06140 - Putative formate acetyltransferase 3 (Pyruvate formate lyase 3), at 1,086,437 to 1,086,991
_06140
OKFHMN_06145: pflD - Pyruvate-formate lyase, at 1,087,069 to 1,088,868
pflD
OKFHMN_06150: ybiV - sugar-phosphatase YbiV, at 1,089,014 to 1,089,829
ybiV
Position (kb)
1087
1088
1089 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1086.319 kb on + strand, within ybiY at 1086.320 kb on - strand, within ybiY at 1086.323 kb on + strand, within ybiY at 1086.503 kb on - strand, within OKFHMN_06140 at 1086.543 kb on - strand, within OKFHMN_06140 at 1086.545 kb on - strand, within OKFHMN_06140 at 1086.555 kb on - strand, within OKFHMN_06140 at 1086.585 kb on + strand, within OKFHMN_06140 at 1086.705 kb on - strand, within OKFHMN_06140 at 1086.706 kb on + strand, within OKFHMN_06140 at 1087.309 kb on + strand, within pflD at 1087.309 kb on + strand, within pflD at 1087.322 kb on - strand, within pflD at 1087.386 kb on + strand, within pflD at 1087.638 kb on - strand, within pflD at 1087.638 kb on - strand, within pflD at 1087.704 kb on + strand, within pflD at 1087.735 kb on + strand, within pflD at 1087.735 kb on + strand, within pflD at 1087.735 kb on + strand, within pflD at 1087.741 kb on + strand, within pflD at 1087.741 kb on + strand, within pflD at 1087.742 kb on - strand, within pflD at 1087.742 kb on - strand, within pflD at 1087.742 kb on - strand, within pflD at 1087.966 kb on + strand, within pflD at 1087.974 kb on + strand, within pflD at 1087.975 kb on - strand, within pflD at 1088.031 kb on + strand, within pflD at 1088.304 kb on + strand, within pflD at 1088.314 kb on - strand, within pflD at 1088.341 kb on + strand, within pflD at 1088.342 kb on - strand, within pflD at 1088.362 kb on + strand, within pflD at 1088.404 kb on + strand, within pflD at 1088.404 kb on + strand, within pflD at 1088.425 kb on - strand, within pflD at 1088.479 kb on - strand, within pflD at 1088.691 kb on + strand at 1088.838 kb on + strand at 1088.838 kb on + strand at 1088.839 kb on - strand at 1088.860 kb on - strand at 1088.860 kb on - strand at 1088.860 kb on - strand at 1088.860 kb on - strand at 1088.866 kb on + strand at 1088.867 kb on - strand at 1088.930 kb on - strand at 1088.972 kb on - strand at 1088.972 kb on - strand at 1088.972 kb on - strand at 1089.013 kb on + strand at 1089.013 kb on + strand at 1089.014 kb on - strand at 1089.021 kb on + strand at 1089.075 kb on - strand at 1089.091 kb on + strand at 1089.092 kb on - strand at 1089.156 kb on - strand, within ybiV at 1089.158 kb on + strand, within ybiV at 1089.187 kb on - strand, within ybiV at 1089.231 kb on - strand, within ybiV at 1089.303 kb on + strand, within ybiV at 1089.351 kb on - strand, within ybiV at 1089.351 kb on - strand, within ybiV at 1089.402 kb on - strand, within ybiV at 1089.513 kb on + strand, within ybiV at 1089.513 kb on + strand, within ybiV at 1089.513 kb on + strand, within ybiV at 1089.514 kb on - strand, within ybiV at 1089.582 kb on + strand, within ybiV at 1089.645 kb on + strand, within ybiV at 1089.760 kb on + strand at 1089.761 kb on - strand at 1089.763 kb on + strand at 1089.821 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,086,319 + ybiY OKFHMN_06135 0.87 +0.5 1,086,320 - ybiY OKFHMN_06135 0.88 -0.1 1,086,323 + ybiY OKFHMN_06135 0.88 +0.1 1,086,503 - OKFHMN_06140 0.12 -0.2 1,086,543 - OKFHMN_06140 0.19 -0.5 1,086,545 - OKFHMN_06140 0.19 -0.1 1,086,555 - OKFHMN_06140 0.21 -0.2 1,086,585 + OKFHMN_06140 0.27 +1.1 1,086,705 - OKFHMN_06140 0.48 -0.8 1,086,706 + OKFHMN_06140 0.48 +0.3 1,087,309 + pflD OKFHMN_06145 0.13 -1.4 1,087,309 + pflD OKFHMN_06145 0.13 -0.6 1,087,322 - pflD OKFHMN_06145 0.14 +0.2 1,087,386 + pflD OKFHMN_06145 0.18 -0.7 1,087,638 - pflD OKFHMN_06145 0.32 -1.4 1,087,638 - pflD OKFHMN_06145 0.32 -1.1 1,087,704 + pflD OKFHMN_06145 0.35 -0.5 1,087,735 + pflD OKFHMN_06145 0.37 -2.3 1,087,735 + pflD OKFHMN_06145 0.37 -0.6 1,087,735 + pflD OKFHMN_06145 0.37 -0.5 1,087,741 + pflD OKFHMN_06145 0.37 -0.3 1,087,741 + pflD OKFHMN_06145 0.37 +0.1 1,087,742 - pflD OKFHMN_06145 0.37 -0.6 1,087,742 - pflD OKFHMN_06145 0.37 -1.7 1,087,742 - pflD OKFHMN_06145 0.37 -0.1 1,087,966 + pflD OKFHMN_06145 0.50 +0.0 1,087,974 + pflD OKFHMN_06145 0.50 +0.3 1,087,975 - pflD OKFHMN_06145 0.50 -2.4 1,088,031 + pflD OKFHMN_06145 0.53 -0.7 1,088,304 + pflD OKFHMN_06145 0.69 -0.7 1,088,314 - pflD OKFHMN_06145 0.69 +1.5 1,088,341 + pflD OKFHMN_06145 0.71 +0.5 1,088,342 - pflD OKFHMN_06145 0.71 +0.0 1,088,362 + pflD OKFHMN_06145 0.72 +0.3 1,088,404 + pflD OKFHMN_06145 0.74 +0.4 1,088,404 + pflD OKFHMN_06145 0.74 +0.6 1,088,425 - pflD OKFHMN_06145 0.75 +2.1 1,088,479 - pflD OKFHMN_06145 0.78 +1.0 1,088,691 + +0.2 1,088,838 + -0.2 1,088,838 + -0.5 1,088,839 - -0.9 1,088,860 - -1.7 1,088,860 - +0.1 1,088,860 - -0.7 1,088,860 - +0.4 1,088,866 + -0.8 1,088,867 - -0.2 1,088,930 - +0.3 1,088,972 - +1.2 1,088,972 - -0.6 1,088,972 - -0.4 1,089,013 + +2.4 1,089,013 + -0.2 1,089,014 - -0.0 1,089,021 + +0.0 1,089,075 - -0.8 1,089,091 + +0.9 1,089,092 - -0.9 1,089,156 - ybiV OKFHMN_06150 0.17 +0.8 1,089,158 + ybiV OKFHMN_06150 0.18 -1.9 1,089,187 - ybiV OKFHMN_06150 0.21 +0.1 1,089,231 - ybiV OKFHMN_06150 0.27 -0.5 1,089,303 + ybiV OKFHMN_06150 0.35 -1.6 1,089,351 - ybiV OKFHMN_06150 0.41 +1.2 1,089,351 - ybiV OKFHMN_06150 0.41 +0.1 1,089,402 - ybiV OKFHMN_06150 0.48 -0.9 1,089,513 + ybiV OKFHMN_06150 0.61 +0.7 1,089,513 + ybiV OKFHMN_06150 0.61 +0.8 1,089,513 + ybiV OKFHMN_06150 0.61 -1.2 1,089,514 - ybiV OKFHMN_06150 0.61 +0.0 1,089,582 + ybiV OKFHMN_06150 0.70 +0.3 1,089,645 + ybiV OKFHMN_06150 0.77 +0.1 1,089,760 + -0.2 1,089,761 - -1.5 1,089,763 + +0.5 1,089,821 - +0.4
Or see this region's nucleotide sequence