Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybjG and deoR are separated by 57 nucleotides deoR and dacC are separated by 46 nucleotides dacC and gstB are separated by 245 nucleotides gstB and yliI overlap by 4 nucleotides
OKFHMN_06030: ybjG - undecaprenyl-diphosphate phosphatase, at 1,064,599 to 1,065,195
ybjG
OKFHMN_06035: deoR - DNA-binding transcriptional repressor DeoR, at 1,065,253 to 1,066,011
deoR
OKFHMN_06040: dacC - serine-type D-Ala-D-Ala carboxypeptidase, at 1,066,058 to 1,067,260
dacC
OKFHMN_06045: gstB - glutathione S-transferase GstB, at 1,067,506 to 1,068,132
gstB
OKFHMN_06050: yliI - aldose sugar dehydrogenase YliI, at 1,068,129 to 1,069,244
yliI
Position (kb)
1066
1067
1068 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1065.069 kb on + strand, within ybjG at 1065.069 kb on + strand, within ybjG at 1065.084 kb on + strand, within ybjG at 1065.239 kb on + strand at 1065.239 kb on + strand at 1065.239 kb on + strand at 1065.239 kb on + strand at 1065.317 kb on + strand at 1065.317 kb on + strand at 1065.448 kb on + strand, within deoR at 1065.574 kb on - strand, within deoR at 1065.623 kb on + strand, within deoR at 1065.798 kb on + strand, within deoR at 1066.017 kb on + strand at 1066.018 kb on - strand at 1066.060 kb on - strand at 1066.187 kb on - strand, within dacC at 1066.342 kb on + strand, within dacC at 1066.343 kb on - strand, within dacC at 1066.343 kb on - strand, within dacC at 1066.529 kb on - strand, within dacC at 1066.600 kb on + strand, within dacC at 1066.646 kb on - strand, within dacC at 1066.759 kb on + strand, within dacC at 1066.760 kb on - strand, within dacC at 1067.040 kb on + strand, within dacC at 1067.041 kb on - strand, within dacC at 1067.297 kb on - strand at 1067.356 kb on - strand at 1067.449 kb on + strand at 1067.511 kb on + strand at 1067.579 kb on + strand, within gstB at 1067.584 kb on + strand, within gstB at 1068.016 kb on + strand, within gstB at 1068.130 kb on + strand at 1068.131 kb on - strand at 1068.154 kb on - strand at 1068.185 kb on - strand at 1068.186 kb on + strand at 1068.187 kb on - strand at 1068.187 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,065,069 + ybjG OKFHMN_06030 0.79 +0.5 1,065,069 + ybjG OKFHMN_06030 0.79 -0.7 1,065,084 + ybjG OKFHMN_06030 0.81 +0.2 1,065,239 + -0.0 1,065,239 + +0.0 1,065,239 + +0.1 1,065,239 + +0.6 1,065,317 + -2.0 1,065,317 + -1.8 1,065,448 + deoR OKFHMN_06035 0.26 -0.4 1,065,574 - deoR OKFHMN_06035 0.42 -0.1 1,065,623 + deoR OKFHMN_06035 0.49 -0.1 1,065,798 + deoR OKFHMN_06035 0.72 -0.3 1,066,017 + +0.2 1,066,018 - +0.3 1,066,060 - +0.0 1,066,187 - dacC OKFHMN_06040 0.11 -2.2 1,066,342 + dacC OKFHMN_06040 0.24 -2.9 1,066,343 - dacC OKFHMN_06040 0.24 -0.8 1,066,343 - dacC OKFHMN_06040 0.24 -1.1 1,066,529 - dacC OKFHMN_06040 0.39 -0.3 1,066,600 + dacC OKFHMN_06040 0.45 +0.0 1,066,646 - dacC OKFHMN_06040 0.49 +0.5 1,066,759 + dacC OKFHMN_06040 0.58 -0.9 1,066,760 - dacC OKFHMN_06040 0.58 -0.7 1,067,040 + dacC OKFHMN_06040 0.82 -0.8 1,067,041 - dacC OKFHMN_06040 0.82 +0.8 1,067,297 - +0.8 1,067,356 - +0.3 1,067,449 + -0.5 1,067,511 + +1.1 1,067,579 + gstB OKFHMN_06045 0.12 +0.6 1,067,584 + gstB OKFHMN_06045 0.12 -0.4 1,068,016 + gstB OKFHMN_06045 0.81 +1.9 1,068,130 + +0.6 1,068,131 - -0.4 1,068,154 - -0.7 1,068,185 - -0.4 1,068,186 + -0.1 1,068,187 - -0.3 1,068,187 - -0.7
Or see this region's nucleotide sequence