Strain Fitness in Escherichia coli ECRC100 around OKFHMN_05515

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpflB and pflA are separated by 191 nucleotidespflA and ycaM are separated by 209 nucleotides OKFHMN_05510: pflB - formate C-acetyltransferase, at 932,971 to 935,253 pflB OKFHMN_05515: pflA - pyruvate formate lyase 1-activating protein, at 935,445 to 936,185 pflA OKFHMN_05520: ycaM - Inner membrane transporter YcaM, at 936,395 to 937,825 ycaM Position (kb) 935 936 937Strain fitness (log2 ratio) -1 0 1 2 3at 934.468 kb on + strand, within pflBat 934.483 kb on + strand, within pflBat 934.715 kb on + strand, within pflBat 934.807 kb on + strand, within pflBat 935.016 kb on + strand, within pflBat 935.047 kb on + strandat 935.047 kb on + strandat 935.223 kb on + strandat 935.247 kb on + strandat 935.247 kb on + strandat 935.247 kb on + strandat 935.273 kb on + strandat 935.347 kb on - strandat 935.347 kb on - strandat 935.351 kb on - strandat 935.351 kb on - strandat 935.351 kb on - strandat 935.386 kb on - strandat 935.514 kb on - strandat 935.597 kb on + strand, within pflAat 935.643 kb on - strand, within pflAat 935.747 kb on + strand, within pflAat 935.747 kb on + strand, within pflAat 936.093 kb on + strand, within pflAat 936.176 kb on - strandat 936.246 kb on - strandat 936.311 kb on + strandat 936.314 kb on - strandat 936.349 kb on - strandat 936.404 kb on - strandat 936.441 kb on - strandat 936.446 kb on + strandat 936.551 kb on - strand, within ycaMat 936.593 kb on - strand, within ycaMat 936.593 kb on - strand, within ycaMat 936.669 kb on - strand, within ycaMat 936.757 kb on + strand, within ycaMat 936.956 kb on - strand, within ycaMat 937.156 kb on - strand, within ycaM

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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934,468 + pflB OKFHMN_05510 0.66 +0.9
934,483 + pflB OKFHMN_05510 0.66 -0.1
934,715 + pflB OKFHMN_05510 0.76 +0.1
934,807 + pflB OKFHMN_05510 0.80 +0.1
935,016 + pflB OKFHMN_05510 0.90 +0.2
935,047 + +0.4
935,047 + +0.4
935,223 + +0.1
935,247 + +0.3
935,247 + +3.0
935,247 + +1.4
935,273 + -1.0
935,347 - +0.4
935,347 - +0.1
935,351 - -1.3
935,351 - -1.1
935,351 - -0.6
935,386 - -1.3
935,514 - +0.1
935,597 + pflA OKFHMN_05515 0.21 -0.7
935,643 - pflA OKFHMN_05515 0.27 -0.0
935,747 + pflA OKFHMN_05515 0.41 +0.8
935,747 + pflA OKFHMN_05515 0.41 +0.5
936,093 + pflA OKFHMN_05515 0.87 +1.2
936,176 - +1.3
936,246 - +0.2
936,311 + -1.1
936,314 - -0.7
936,349 - -0.6
936,404 - +0.1
936,441 - -1.4
936,446 + +0.7
936,551 - ycaM OKFHMN_05520 0.11 +0.1
936,593 - ycaM OKFHMN_05520 0.14 -0.1
936,593 - ycaM OKFHMN_05520 0.14 +0.5
936,669 - ycaM OKFHMN_05520 0.19 +0.9
936,757 + ycaM OKFHMN_05520 0.25 +0.8
936,956 - ycaM OKFHMN_05520 0.39 -1.1
937,156 - ycaM OKFHMN_05520 0.53 +0.4

Or see this region's nucleotide sequence