Strain Fitness in Escherichia coli ECRC100 around OKFHMN_05200

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthspQ and yccU are separated by 44 nucleotidesyccU and yccT are separated by 172 nucleotidesyccT and mgsA are separated by 95 nucleotidesmgsA and helD are separated by 31 nucleotides OKFHMN_05190: hspQ - heat shock protein HspQ, at 857,976 to 858,293 hspQ OKFHMN_05195: yccU - Uncharacterized protein YccU, at 858,338 to 858,751 yccU OKFHMN_05200: yccT - DUF2057 domain-containing protein, at 858,924 to 859,586 yccT OKFHMN_05205: mgsA - methylglyoxal synthase, at 859,682 to 860,140 mgsA OKFHMN_05210: helD - DNA helicase IV, at 860,172 to 862,226 helD Position (kb) 858 859 860Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 857.942 kb on + strandat 857.944 kb on + strandat 858.170 kb on + strand, within hspQat 858.170 kb on + strand, within hspQat 858.174 kb on + strand, within hspQat 858.193 kb on + strand, within hspQat 858.340 kb on - strandat 858.482 kb on - strand, within yccUat 858.648 kb on + strand, within yccUat 858.649 kb on - strand, within yccUat 858.723 kb on - strandat 858.843 kb on + strandat 858.844 kb on - strandat 858.849 kb on + strandat 858.849 kb on + strandat 858.849 kb on + strandat 858.850 kb on - strandat 858.852 kb on - strandat 858.860 kb on - strandat 858.919 kb on + strandat 859.152 kb on + strand, within yccTat 859.155 kb on - strand, within yccTat 859.353 kb on + strand, within yccTat 859.354 kb on - strand, within yccTat 859.375 kb on - strand, within yccTat 859.548 kb on - strandat 859.635 kb on + strandat 859.644 kb on - strandat 859.648 kb on + strandat 859.649 kb on - strandat 859.656 kb on - strandat 859.676 kb on + strandat 859.721 kb on - strandat 859.788 kb on + strand, within mgsAat 859.803 kb on + strand, within mgsAat 859.833 kb on + strand, within mgsAat 859.959 kb on + strand, within mgsAat 860.288 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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857,942 + -0.3
857,944 + +0.2
858,170 + hspQ OKFHMN_05190 0.61 +0.2
858,170 + hspQ OKFHMN_05190 0.61 -0.7
858,174 + hspQ OKFHMN_05190 0.62 +0.2
858,193 + hspQ OKFHMN_05190 0.68 +0.5
858,340 - -0.7
858,482 - yccU OKFHMN_05195 0.35 +0.0
858,648 + yccU OKFHMN_05195 0.75 +0.4
858,649 - yccU OKFHMN_05195 0.75 -0.0
858,723 - +0.6
858,843 + -3.4
858,844 - +0.5
858,849 + -2.5
858,849 + -0.1
858,849 + +0.4
858,850 - -0.4
858,852 - +0.5
858,860 - -2.4
858,919 + +0.4
859,152 + yccT OKFHMN_05200 0.34 +0.4
859,155 - yccT OKFHMN_05200 0.35 -0.3
859,353 + yccT OKFHMN_05200 0.65 -0.6
859,354 - yccT OKFHMN_05200 0.65 -0.0
859,375 - yccT OKFHMN_05200 0.68 -1.8
859,548 - -1.4
859,635 + +1.1
859,644 - +0.1
859,648 + +0.1
859,649 - +2.4
859,656 - +0.2
859,676 + -0.6
859,721 - -1.4
859,788 + mgsA OKFHMN_05205 0.23 +1.2
859,803 + mgsA OKFHMN_05205 0.26 +2.3
859,833 + mgsA OKFHMN_05205 0.33 +0.1
859,959 + mgsA OKFHMN_05205 0.60 -0.4
860,288 - +0.6

Or see this region's nucleotide sequence