Strain Fitness in Escherichia coli ECRC100 around OKFHMN_03850

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcgsD and cgsE are separated by 4 nucleotidescgsE and cgsF are separated by 24 nucleotidescgsF and cgsG are separated by 26 nucleotides OKFHMN_03845: cgsD - transcriptional regulator CsgD, at 613,771 to 614,421 cgsD OKFHMN_03850: cgsE - curli production assembly/transport protein CsgE, at 614,426 to 614,815 cgsE OKFHMN_03855: cgsF - curli production assembly/transport protein CsgF, at 614,840 to 615,256 cgsF OKFHMN_03860: cgsG - curli production assembly/transport protein CsgG, at 615,283 to 616,116 cgsG Position (kb) 614 615Strain fitness (log2 ratio) -1 0 1at 614.206 kb on + strand, within cgsDat 614.527 kb on + strand, within cgsEat 614.601 kb on + strand, within cgsEat 615.139 kb on + strand, within cgsFat 615.254 kb on + strandat 615.443 kb on - strand, within cgsGat 615.687 kb on + strand, within cgsG

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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614,206 + cgsD OKFHMN_03845 0.67 -0.6
614,527 + cgsE OKFHMN_03850 0.26 -1.0
614,601 + cgsE OKFHMN_03850 0.45 -1.5
615,139 + cgsF OKFHMN_03855 0.72 -0.0
615,254 + -1.6
615,443 - cgsG OKFHMN_03860 0.19 +0.5
615,687 + cgsG OKFHMN_03860 0.48 -1.6

Or see this region's nucleotide sequence