Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nagZ and thiK are separated by 10 nucleotides thiK and lpoB overlap by 20 nucleotides lpoB and OKFHMN_03520 are separated by 13 nucleotides
OKFHMN_03505: nagZ - beta-N-acetylhexosaminidase, at 551,886 to 552,911
nagZ
OKFHMN_03510: thiK - thiamine kinase, at 552,922 to 553,746
thiK
OKFHMN_03515: lpoB - penicillin-binding protein activator LpoB, at 553,727 to 554,368
lpoB
OKFHMN_03520: OKFHMN_03520 - DUF1425 domain-containing protein, at 554,382 to 554,759
_03520
Position (kb) 552
553
554 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 551.923 kb on - strand at 551.997 kb on - strand, within nagZ at 552.266 kb on - strand, within nagZ at 552.285 kb on - strand, within nagZ at 552.536 kb on + strand, within nagZ at 552.806 kb on - strand, within nagZ at 552.932 kb on - strand at 553.064 kb on - strand, within thiK at 553.098 kb on - strand, within thiK at 553.246 kb on - strand, within thiK at 553.259 kb on - strand, within thiK at 553.259 kb on - strand, within thiK at 553.413 kb on - strand, within thiK at 553.416 kb on - strand, within thiK at 553.416 kb on - strand, within thiK at 553.440 kb on + strand, within thiK at 553.505 kb on - strand, within thiK at 553.505 kb on - strand, within thiK at 553.659 kb on + strand, within thiK at 553.663 kb on - strand, within thiK at 553.800 kb on - strand, within lpoB at 553.883 kb on - strand, within lpoB at 553.963 kb on + strand, within lpoB at 554.389 kb on + strand at 554.390 kb on - strand at 554.390 kb on - strand at 554.392 kb on - strand at 554.519 kb on - strand, within OKFHMN_03520 at 554.719 kb on - strand, within OKFHMN_03520
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 551,923 - -0.5 551,997 - nagZ OKFHMN_03505 0.11 -1.2 552,266 - nagZ OKFHMN_03505 0.37 +0.5 552,285 - nagZ OKFHMN_03505 0.39 +1.4 552,536 + nagZ OKFHMN_03505 0.63 +1.0 552,806 - nagZ OKFHMN_03505 0.90 +0.8 552,932 - -0.5 553,064 - thiK OKFHMN_03510 0.17 -1.0 553,098 - thiK OKFHMN_03510 0.21 -3.0 553,246 - thiK OKFHMN_03510 0.39 -1.0 553,259 - thiK OKFHMN_03510 0.41 +0.3 553,259 - thiK OKFHMN_03510 0.41 +0.2 553,413 - thiK OKFHMN_03510 0.60 -0.7 553,416 - thiK OKFHMN_03510 0.60 -4.5 553,416 - thiK OKFHMN_03510 0.60 +1.1 553,440 + thiK OKFHMN_03510 0.63 +0.3 553,505 - thiK OKFHMN_03510 0.71 +0.5 553,505 - thiK OKFHMN_03510 0.71 -0.0 553,659 + thiK OKFHMN_03510 0.89 -1.7 553,663 - thiK OKFHMN_03510 0.90 +0.7 553,800 - lpoB OKFHMN_03515 0.11 +0.0 553,883 - lpoB OKFHMN_03515 0.24 -0.2 553,963 + lpoB OKFHMN_03515 0.37 -0.9 554,389 + -0.2 554,390 - -1.6 554,390 - -2.0 554,392 - +0.9 554,519 - OKFHMN_03520 0.36 +0.4 554,719 - OKFHMN_03520 0.89 -0.0
Or see this region's nucleotide sequence