Strain Fitness in Escherichia coli ECRC100 around OKFHMN_02675

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttrpB and trpA overlap by 1 nucleotidestrpA and yciG are separated by 380 nucleotidesyciG and yciF are separated by 85 nucleotides OKFHMN_02670: trpB - tryptophan synthase subunit beta, at 425,480 to 426,673 trpB OKFHMN_02675: trpA - tryptophan synthase subunit alpha, at 426,673 to 427,479 trpA OKFHMN_02680: yciG - Uncharacterized protein YciG, at 427,860 to 428,039 yciG OKFHMN_02685: yciF - Protein YciF, at 428,125 to 428,625 yciF Position (kb) 426 427 428Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 425.876 kb on + strand, within trpBat 425.877 kb on - strand, within trpBat 425.877 kb on - strand, within trpBat 425.877 kb on - strand, within trpBat 426.035 kb on + strand, within trpBat 426.036 kb on - strand, within trpBat 426.422 kb on + strand, within trpBat 426.591 kb on + strandat 426.791 kb on + strand, within trpAat 426.792 kb on - strand, within trpAat 426.977 kb on - strand, within trpAat 427.190 kb on - strand, within trpAat 427.280 kb on - strand, within trpAat 428.068 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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425,876 + trpB OKFHMN_02670 0.33 -3.0
425,877 - trpB OKFHMN_02670 0.33 -1.6
425,877 - trpB OKFHMN_02670 0.33 -3.2
425,877 - trpB OKFHMN_02670 0.33 -3.5
426,035 + trpB OKFHMN_02670 0.46 -2.7
426,036 - trpB OKFHMN_02670 0.47 -3.5
426,422 + trpB OKFHMN_02670 0.79 -0.7
426,591 + -1.2
426,791 + trpA OKFHMN_02675 0.15 -3.1
426,792 - trpA OKFHMN_02675 0.15 -3.9
426,977 - trpA OKFHMN_02675 0.38 -3.0
427,190 - trpA OKFHMN_02675 0.64 -2.3
427,280 - trpA OKFHMN_02675 0.75 -4.3
428,068 + -0.2

Or see this region's nucleotide sequence