Strain Fitness in Escherichia coli ECRC100 around OKFHMN_02665

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttrpD and trpCF are separated by 3 nucleotidestrpCF and trpB are separated by 11 nucleotides OKFHMN_02660: trpD - bifunctional anthranilate synthase glutamate amidotransferase component TrpG/anthranilate phosphoribosyltransferase TrpD, at 422,511 to 424,106 trpD OKFHMN_02665: trpCF - bifunctional indole-3-glycerol-phosphate synthase TrpC/phosphoribosylanthranilate isomerase TrpF, at 424,110 to 425,468 trpCF OKFHMN_02670: trpB - tryptophan synthase subunit beta, at 425,480 to 426,673 trpB Position (kb) 424 425 426Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 423.492 kb on - strand, within trpDat 423.689 kb on + strand, within trpDat 423.724 kb on + strand, within trpDat 424.479 kb on + strand, within trpCFat 424.763 kb on + strand, within trpCFat 424.810 kb on + strand, within trpCFat 424.885 kb on + strand, within trpCFat 424.886 kb on - strand, within trpCFat 424.941 kb on + strand, within trpCFat 424.941 kb on + strand, within trpCFat 425.078 kb on + strand, within trpCFat 425.078 kb on + strand, within trpCFat 425.078 kb on + strand, within trpCFat 425.387 kb on + strandat 425.466 kb on + strandat 425.525 kb on + strandat 425.876 kb on + strand, within trpBat 425.877 kb on - strand, within trpBat 425.877 kb on - strand, within trpBat 425.877 kb on - strand, within trpBat 426.035 kb on + strand, within trpBat 426.036 kb on - strand, within trpBat 426.422 kb on + strand, within trpB

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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423,492 - trpD OKFHMN_02660 0.61 -3.8
423,689 + trpD OKFHMN_02660 0.74 -1.3
423,724 + trpD OKFHMN_02660 0.76 -1.5
424,479 + trpCF OKFHMN_02665 0.27 -1.2
424,763 + trpCF OKFHMN_02665 0.48 +2.8
424,810 + trpCF OKFHMN_02665 0.52 -2.7
424,885 + trpCF OKFHMN_02665 0.57 -2.2
424,886 - trpCF OKFHMN_02665 0.57 -0.8
424,941 + trpCF OKFHMN_02665 0.61 -1.5
424,941 + trpCF OKFHMN_02665 0.61 -2.1
425,078 + trpCF OKFHMN_02665 0.71 -3.7
425,078 + trpCF OKFHMN_02665 0.71 -2.1
425,078 + trpCF OKFHMN_02665 0.71 -2.4
425,387 + -3.1
425,466 + +0.4
425,525 + -3.2
425,876 + trpB OKFHMN_02670 0.33 -3.0
425,877 - trpB OKFHMN_02670 0.33 -1.6
425,877 - trpB OKFHMN_02670 0.33 -3.2
425,877 - trpB OKFHMN_02670 0.33 -3.5
426,035 + trpB OKFHMN_02670 0.46 -2.7
426,036 - trpB OKFHMN_02670 0.47 -3.5
426,422 + trpB OKFHMN_02670 0.79 -0.7

Or see this region's nucleotide sequence