Strain Fitness in Escherichia coli ECRC100 around OKFHMN_02660

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttrpE and trpD overlap by 1 nucleotidestrpD and trpCF are separated by 3 nucleotides OKFHMN_02655: trpE - anthranilate synthase component I, at 420,949 to 422,511 trpE OKFHMN_02660: trpD - bifunctional anthranilate synthase glutamate amidotransferase component TrpG/anthranilate phosphoribosyltransferase TrpD, at 422,511 to 424,106 trpD OKFHMN_02665: trpCF - bifunctional indole-3-glycerol-phosphate synthase TrpC/phosphoribosylanthranilate isomerase TrpF, at 424,110 to 425,468 trpCF Position (kb) 422 423 424 425Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 421.792 kb on + strand, within trpEat 421.809 kb on + strand, within trpEat 421.822 kb on + strand, within trpEat 421.823 kb on - strand, within trpEat 422.126 kb on - strand, within trpEat 422.126 kb on - strand, within trpEat 422.132 kb on - strand, within trpEat 422.258 kb on + strand, within trpEat 422.259 kb on - strand, within trpEat 422.589 kb on - strandat 422.708 kb on + strand, within trpDat 423.492 kb on - strand, within trpDat 423.689 kb on + strand, within trpDat 423.724 kb on + strand, within trpDat 424.338 kb on + strand, within trpCFat 424.479 kb on + strand, within trpCFat 424.810 kb on + strand, within trpCFat 424.885 kb on + strand, within trpCFat 424.886 kb on - strand, within trpCFat 424.941 kb on + strand, within trpCFat 424.941 kb on + strand, within trpCFat 425.078 kb on + strand, within trpCFat 425.078 kb on + strand, within trpCFat 425.078 kb on + strand, within trpCF

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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421,792 + trpE OKFHMN_02655 0.54 -1.4
421,809 + trpE OKFHMN_02655 0.55 -2.3
421,822 + trpE OKFHMN_02655 0.56 +0.1
421,823 - trpE OKFHMN_02655 0.56 -3.2
422,126 - trpE OKFHMN_02655 0.75 -1.5
422,126 - trpE OKFHMN_02655 0.75 -2.9
422,132 - trpE OKFHMN_02655 0.76 -6.0
422,258 + trpE OKFHMN_02655 0.84 +0.3
422,259 - trpE OKFHMN_02655 0.84 -1.2
422,589 - -1.7
422,708 + trpD OKFHMN_02660 0.12 -1.7
423,492 - trpD OKFHMN_02660 0.61 -3.8
423,689 + trpD OKFHMN_02660 0.74 -1.3
423,724 + trpD OKFHMN_02660 0.76 -1.5
424,338 + trpCF OKFHMN_02665 0.17 -0.7
424,479 + trpCF OKFHMN_02665 0.27 -1.2
424,810 + trpCF OKFHMN_02665 0.52 -2.7
424,885 + trpCF OKFHMN_02665 0.57 -2.2
424,886 - trpCF OKFHMN_02665 0.57 -0.8
424,941 + trpCF OKFHMN_02665 0.61 -1.5
424,941 + trpCF OKFHMN_02665 0.61 -2.1
425,078 + trpCF OKFHMN_02665 0.71 -3.7
425,078 + trpCF OKFHMN_02665 0.71 -2.1
425,078 + trpCF OKFHMN_02665 0.71 -2.4

Or see this region's nucleotide sequence