Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt curA and mddA are separated by 180 nucleotides mddA and OKFHMN_01540 overlap by 4 nucleotides OKFHMN_01540 and ydcY are separated by 0 nucleotides ydcY and ortT are separated by 85 nucleotides ortT and patD are separated by 386 nucleotides
OKFHMN_01530: curA - NADPH-dependent curcumin/dihydrocurcumin reductase, at 215,239 to 216,276
curA
OKFHMN_01535: mddA - L-methionine sulfoximine/L-methionine sulfone acetyltransferase, at 216,457 to 216,975
mddA
OKFHMN_01540: OKFHMN_01540 - Putative inner membrane exporter, YdcZ, at 216,972 to 217,421
_01540
OKFHMN_01545: ydcY - Uncharacterized protein YdcY, at 217,422 to 217,655
ydcY
OKFHMN_01550: ortT - orphan toxin OrtT, at 217,741 to 217,914
ortT
OKFHMN_01555: patD - aminobutyraldehyde dehydrogenase, at 218,301 to 219,725
patD
Position (kb) 216
217
218 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 216.087 kb on - strand, within curA at 216.106 kb on - strand, within curA at 216.315 kb on + strand at 216.527 kb on - strand, within mddA at 216.529 kb on - strand, within mddA at 216.599 kb on + strand, within mddA at 216.750 kb on + strand, within mddA at 217.068 kb on - strand, within OKFHMN_01540 at 217.194 kb on - strand, within OKFHMN_01540 at 217.420 kb on - strand at 218.140 kb on + strand at 218.174 kb on + strand at 218.360 kb on + strand at 218.361 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 216,087 - curA OKFHMN_01530 0.82 +2.1 216,106 - curA OKFHMN_01530 0.84 +0.0 216,315 + -1.1 216,527 - mddA OKFHMN_01535 0.13 +1.2 216,529 - mddA OKFHMN_01535 0.14 -0.5 216,599 + mddA OKFHMN_01535 0.27 -2.2 216,750 + mddA OKFHMN_01535 0.56 -3.5 217,068 - OKFHMN_01540 0.21 +0.7 217,194 - OKFHMN_01540 0.49 -1.4 217,420 - +0.3 218,140 + +0.0 218,174 + -0.9 218,360 + +1.1 218,361 - -0.3
Or see this region's nucleotide sequence