Strain Fitness in Escherichia coli ECRC100 around OKFHMN_01145

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyneE and tam are separated by 3 nucleotidestam and lsrG are separated by 56 nucleotideslsrG and lsrF are separated by 23 nucleotideslsrF and lsrB are separated by 26 nucleotides OKFHMN_01135: yneE - UPF0187 protein YneE, at 115,662 to 116,576 yneE OKFHMN_01140: tam - trans-aconitate 2-methyltransferase, at 116,580 to 117,338 tam OKFHMN_01145: lsrG - (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase, at 117,395 to 117,685 lsrG OKFHMN_01150: lsrF - 3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase, at 117,709 to 118,584 lsrF OKFHMN_01155: lsrB - autoinducer 2 ABC transporter substrate-binding protein LsrB, at 118,611 to 119,633 lsrB Position (kb) 117 118Strain fitness (log2 ratio) -1 0 1at 117.313 kb on + strandat 117.315 kb on + strandat 117.491 kb on - strand, within lsrGat 117.729 kb on - strandat 117.738 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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117,313 + +0.4
117,315 + +0.7
117,491 - lsrG OKFHMN_01145 0.33 -0.2
117,729 - -0.2
117,738 - +0.8

Or see this region's nucleotide sequence