Strain Fitness in Escherichia coli ECRC100 around OKFHMN_00625

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpotB and potA overlap by 17 nucleotidespotA and pepT are separated by 249 nucleotides OKFHMN_00620: potB - spermidine/putrescine ABC transporter permease PotB, at 30,577 to 31,440 potB OKFHMN_00625: potA - spermidine/putrescine ABC transporter ATP-binding protein PotA, at 31,424 to 32,560 potA OKFHMN_00630: pepT - peptidase T, at 32,810 to 34,036 pepT Position (kb) 31 32 33Strain fitness (log2 ratio) -3 -2 -1 0 1at 30.716 kb on - strand, within potBat 30.774 kb on - strand, within potBat 30.866 kb on - strand, within potBat 31.284 kb on - strand, within potBat 31.284 kb on - strand, within potBat 31.399 kb on - strandat 31.964 kb on - strand, within potAat 31.976 kb on - strand, within potAat 32.563 kb on - strandat 32.685 kb on + strandat 32.777 kb on - strandat 33.101 kb on + strand, within pepTat 33.208 kb on + strand, within pepTat 33.219 kb on + strand, within pepTat 33.241 kb on + strand, within pepT

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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30,716 - potB OKFHMN_00620 0.16 +0.1
30,774 - potB OKFHMN_00620 0.23 -0.6
30,866 - potB OKFHMN_00620 0.33 -2.4
31,284 - potB OKFHMN_00620 0.82 +0.5
31,284 - potB OKFHMN_00620 0.82 +0.2
31,399 - +0.1
31,964 - potA OKFHMN_00625 0.47 -0.5
31,976 - potA OKFHMN_00625 0.49 -0.3
32,563 - +0.6
32,685 + -0.7
32,777 - +0.7
33,101 + pepT OKFHMN_00630 0.24 +0.9
33,208 + pepT OKFHMN_00630 0.32 -1.3
33,219 + pepT OKFHMN_00630 0.33 -3.7
33,241 + pepT OKFHMN_00630 0.35 -3.1

Or see this region's nucleotide sequence