Strain Fitness in Escherichia coli ECRC100 around OKFHMN_00615

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttnp-IS3 and OKFHMN_00605 are separated by 10 nucleotidesOKFHMN_00605 and OKFHMN_00610 are separated by 141 nucleotidesOKFHMN_00610 and espK are separated by 188 nucleotidesespK and potB are separated by 363 nucleotides OKFHMN_00600: tnp-IS3 - IS3 family transposase, at 27,817 to 27,978 tnp-IS3 OKFHMN_00605: OKFHMN_00605 - Mutator family transposase, at 27,989 to 28,288 _00605 OKFHMN_00610: OKFHMN_00610 - hypothetical protein, at 28,430 to 28,663 _00610 OKFHMN_00615: espK - type III secretion system effector EspK, at 28,852 to 30,213 espK OKFHMN_00620: potB - spermidine/putrescine ABC transporter permease PotB, at 30,577 to 31,440 potB Position (kb) 28 29 30 31Strain fitness (log2 ratio) -2 -1 0 1at 28.864 kb on - strandat 29.640 kb on + strand, within espKat 29.781 kb on + strand, within espKat 30.716 kb on - strand, within potBat 30.774 kb on - strand, within potBat 30.866 kb on - strand, within potB

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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28,864 - -0.3
29,640 + espK OKFHMN_00615 0.58 +0.1
29,781 + espK OKFHMN_00615 0.68 +0.2
30,716 - potB OKFHMN_00620 0.16 +0.1
30,774 - potB OKFHMN_00620 0.23 -0.6
30,866 - potB OKFHMN_00620 0.33 -2.4

Or see this region's nucleotide sequence