Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt astC and astA overlap by 4 nucleotides astA and astD overlap by 4 nucleotides
OKFHMN_26905: astC - succinylornithine/acetylornithine transaminase, at 5,178,925 to 5,180,145
astC
OKFHMN_26910: astA - arginine N-succinyltransferase, at 5,180,142 to 5,181,176
astA
OKFHMN_26915: astD - succinylglutamate-semialdehyde dehydrogenase, at 5,181,173 to 5,182,651
astD
Position (kb) 5180
5181
5182 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5179.326 kb on + strand, within astC at 5179.432 kb on + strand, within astC at 5179.434 kb on + strand, within astC at 5179.630 kb on + strand, within astC at 5179.630 kb on + strand, within astC at 5179.630 kb on + strand, within astC at 5179.631 kb on - strand, within astC at 5179.631 kb on - strand, within astC at 5179.631 kb on - strand, within astC at 5179.636 kb on + strand, within astC at 5179.637 kb on - strand, within astC at 5179.638 kb on + strand, within astC at 5180.451 kb on + strand, within astA at 5180.576 kb on + strand, within astA at 5180.606 kb on + strand, within astA at 5180.606 kb on + strand, within astA at 5180.655 kb on + strand, within astA at 5180.824 kb on + strand, within astA at 5180.824 kb on + strand, within astA at 5180.886 kb on + strand, within astA at 5180.887 kb on - strand, within astA at 5180.887 kb on - strand, within astA at 5180.951 kb on + strand, within astA at 5181.232 kb on + strand at 5181.604 kb on + strand, within astD at 5181.667 kb on + strand, within astD
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 5,179,326 + astC OKFHMN_26905 0.33 +0.8 5,179,432 + astC OKFHMN_26905 0.42 +0.9 5,179,434 + astC OKFHMN_26905 0.42 +0.2 5,179,630 + astC OKFHMN_26905 0.58 +0.5 5,179,630 + astC OKFHMN_26905 0.58 +0.3 5,179,630 + astC OKFHMN_26905 0.58 -0.2 5,179,631 - astC OKFHMN_26905 0.58 +0.2 5,179,631 - astC OKFHMN_26905 0.58 +0.3 5,179,631 - astC OKFHMN_26905 0.58 -0.4 5,179,636 + astC OKFHMN_26905 0.58 -0.4 5,179,637 - astC OKFHMN_26905 0.58 -2.0 5,179,638 + astC OKFHMN_26905 0.58 -0.0 5,180,451 + astA OKFHMN_26910 0.30 -0.5 5,180,576 + astA OKFHMN_26910 0.42 +0.4 5,180,606 + astA OKFHMN_26910 0.45 -2.8 5,180,606 + astA OKFHMN_26910 0.45 +1.8 5,180,655 + astA OKFHMN_26910 0.50 +0.8 5,180,824 + astA OKFHMN_26910 0.66 +0.8 5,180,824 + astA OKFHMN_26910 0.66 -0.0 5,180,886 + astA OKFHMN_26910 0.72 +0.5 5,180,887 - astA OKFHMN_26910 0.72 +0.6 5,180,887 - astA OKFHMN_26910 0.72 -0.1 5,180,951 + astA OKFHMN_26910 0.78 -0.0 5,181,232 + -1.0 5,181,604 + astD OKFHMN_26915 0.29 +0.4 5,181,667 + astD OKFHMN_26915 0.33 -1.0
Or see this region's nucleotide sequence