Strain Fitness in Escherichia coli ECRC100 around OKFHMN_26680

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnimT and nimR are separated by 99 nucleotidesnimR and yeaL overlap by 44 nucleotidesyeaL and yeaK are separated by 274 nucleotides OKFHMN_26675: nimT - 2-nitroimidazole transporter, at 5,131,960 to 5,133,141 nimT OKFHMN_26680: nimR - DNA-binding transcriptional regulator NimR, at 5,133,241 to 5,134,059 nimR OKFHMN_26685: yeaL - UPF0756 membrane protein CKO_01811, at 5,134,016 to 5,134,462 yeaL OKFHMN_26690: yeaK - mischarged aminoacyl-tRNA deacylase, at 5,134,737 to 5,135,240 yeaK Position (kb) 5133 5134 5135Strain fitness (log2 ratio) -2 -1 0 1 2 3at 5132.307 kb on + strand, within nimTat 5132.459 kb on + strand, within nimTat 5132.675 kb on - strand, within nimTat 5132.780 kb on - strand, within nimTat 5132.833 kb on + strand, within nimTat 5133.377 kb on + strand, within nimRat 5133.465 kb on + strand, within nimRat 5133.866 kb on + strand, within nimRat 5133.963 kb on + strand, within nimRat 5133.963 kb on + strand, within nimRat 5133.969 kb on + strand, within nimRat 5134.002 kb on + strandat 5134.003 kb on - strandat 5134.735 kb on - strandat 5134.984 kb on - strand, within yeaK

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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5,132,307 + nimT OKFHMN_26675 0.29 -0.1
5,132,459 + nimT OKFHMN_26675 0.42 +0.5
5,132,675 - nimT OKFHMN_26675 0.60 +1.0
5,132,780 - nimT OKFHMN_26675 0.69 -0.2
5,132,833 + nimT OKFHMN_26675 0.74 -0.1
5,133,377 + nimR OKFHMN_26680 0.17 -2.6
5,133,465 + nimR OKFHMN_26680 0.27 +1.2
5,133,866 + nimR OKFHMN_26680 0.76 +1.0
5,133,963 + nimR OKFHMN_26680 0.88 -1.7
5,133,963 + nimR OKFHMN_26680 0.88 -0.3
5,133,969 + nimR OKFHMN_26680 0.89 +1.3
5,134,002 + +1.2
5,134,003 - +3.4
5,134,735 - -1.5
5,134,984 - yeaK OKFHMN_26690 0.49 -0.2

Or see this region's nucleotide sequence