Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wzzB and hisIE are separated by 96 nucleotides hisIE and hisF overlap by 7 nucleotides hisF and hisA overlap by 19 nucleotides
OKFHMN_24480: wzzB - LPS O-antigen chain length determinant protein WzzB, at 4,785,430 to 4,786,407
wzzB
OKFHMN_24485: hisIE - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 4,786,504 to 4,787,115
hisIE
OKFHMN_24490: hisF - Imidazole glycerol phosphate synthase subunit HisF, at 4,787,109 to 4,787,885
hisF
OKFHMN_24495: hisA - 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)me thylideneamino]imidazole-4-carboxamide isomerase, at 4,787,867 to 4,788,604
hisA
Position (kb)
4786
4787
4788 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 4785.646 kb on + strand, within wzzB at 4785.646 kb on + strand, within wzzB at 4785.670 kb on + strand, within wzzB at 4785.746 kb on + strand, within wzzB at 4785.763 kb on - strand, within wzzB at 4785.835 kb on + strand, within wzzB at 4785.835 kb on + strand, within wzzB at 4785.880 kb on + strand, within wzzB at 4785.880 kb on + strand, within wzzB at 4786.107 kb on + strand, within wzzB at 4786.149 kb on + strand, within wzzB at 4786.150 kb on - strand, within wzzB at 4786.150 kb on - strand, within wzzB at 4786.189 kb on + strand, within wzzB at 4786.258 kb on + strand, within wzzB at 4786.269 kb on - strand, within wzzB at 4786.481 kb on - strand at 4786.628 kb on - strand, within hisIE at 4786.628 kb on - strand, within hisIE at 4786.701 kb on - strand, within hisIE at 4786.891 kb on + strand, within hisIE at 4786.895 kb on - strand, within hisIE at 4787.009 kb on + strand, within hisIE at 4787.514 kb on - strand, within hisF at 4788.046 kb on - strand, within hisA at 4788.046 kb on - strand, within hisA
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 4,785,646 + wzzB OKFHMN_24480 0.22 -0.6 4,785,646 + wzzB OKFHMN_24480 0.22 +0.0 4,785,670 + wzzB OKFHMN_24480 0.25 +1.6 4,785,746 + wzzB OKFHMN_24480 0.32 -0.4 4,785,763 - wzzB OKFHMN_24480 0.34 -3.0 4,785,835 + wzzB OKFHMN_24480 0.41 +0.1 4,785,835 + wzzB OKFHMN_24480 0.41 +0.9 4,785,880 + wzzB OKFHMN_24480 0.46 -0.2 4,785,880 + wzzB OKFHMN_24480 0.46 -0.5 4,786,107 + wzzB OKFHMN_24480 0.69 -0.6 4,786,149 + wzzB OKFHMN_24480 0.74 -1.4 4,786,150 - wzzB OKFHMN_24480 0.74 -0.1 4,786,150 - wzzB OKFHMN_24480 0.74 +0.2 4,786,189 + wzzB OKFHMN_24480 0.78 -1.5 4,786,258 + wzzB OKFHMN_24480 0.85 -1.7 4,786,269 - wzzB OKFHMN_24480 0.86 -0.5 4,786,481 - -0.4 4,786,628 - hisIE OKFHMN_24485 0.20 -2.9 4,786,628 - hisIE OKFHMN_24485 0.20 -3.4 4,786,701 - hisIE OKFHMN_24485 0.32 -3.3 4,786,891 + hisIE OKFHMN_24485 0.63 -2.4 4,786,895 - hisIE OKFHMN_24485 0.64 -3.6 4,787,009 + hisIE OKFHMN_24485 0.83 -5.0 4,787,514 - hisF OKFHMN_24490 0.52 -3.1 4,788,046 - hisA OKFHMN_24495 0.24 -5.4 4,788,046 - hisA OKFHMN_24495 0.24 -2.6
Or see this region's nucleotide sequence