Strain Fitness in Escherichia coli ECRC100 around OKFHMN_24485

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwzzB and hisIE are separated by 96 nucleotideshisIE and hisF overlap by 7 nucleotideshisF and hisA overlap by 19 nucleotides OKFHMN_24480: wzzB - LPS O-antigen chain length determinant protein WzzB, at 4,785,430 to 4,786,407 wzzB OKFHMN_24485: hisIE - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 4,786,504 to 4,787,115 hisIE OKFHMN_24490: hisF - Imidazole glycerol phosphate synthase subunit HisF, at 4,787,109 to 4,787,885 hisF OKFHMN_24495: hisA - 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)me thylideneamino]imidazole-4-carboxamide isomerase, at 4,787,867 to 4,788,604 hisA Position (kb) 4786 4787 4788Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 4785.646 kb on + strand, within wzzBat 4785.646 kb on + strand, within wzzBat 4785.670 kb on + strand, within wzzBat 4785.746 kb on + strand, within wzzBat 4785.763 kb on - strand, within wzzBat 4785.835 kb on + strand, within wzzBat 4785.835 kb on + strand, within wzzBat 4785.880 kb on + strand, within wzzBat 4785.880 kb on + strand, within wzzBat 4786.107 kb on + strand, within wzzBat 4786.149 kb on + strand, within wzzBat 4786.150 kb on - strand, within wzzBat 4786.150 kb on - strand, within wzzBat 4786.189 kb on + strand, within wzzBat 4786.258 kb on + strand, within wzzBat 4786.269 kb on - strand, within wzzBat 4786.481 kb on - strandat 4786.628 kb on - strand, within hisIEat 4786.628 kb on - strand, within hisIEat 4786.701 kb on - strand, within hisIEat 4786.891 kb on + strand, within hisIEat 4786.895 kb on - strand, within hisIEat 4787.009 kb on + strand, within hisIEat 4787.514 kb on - strand, within hisFat 4788.046 kb on - strand, within hisAat 4788.046 kb on - strand, within hisA

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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4,785,646 + wzzB OKFHMN_24480 0.22 -0.6
4,785,646 + wzzB OKFHMN_24480 0.22 +0.0
4,785,670 + wzzB OKFHMN_24480 0.25 +1.6
4,785,746 + wzzB OKFHMN_24480 0.32 -0.4
4,785,763 - wzzB OKFHMN_24480 0.34 -3.0
4,785,835 + wzzB OKFHMN_24480 0.41 +0.1
4,785,835 + wzzB OKFHMN_24480 0.41 +0.9
4,785,880 + wzzB OKFHMN_24480 0.46 -0.2
4,785,880 + wzzB OKFHMN_24480 0.46 -0.5
4,786,107 + wzzB OKFHMN_24480 0.69 -0.6
4,786,149 + wzzB OKFHMN_24480 0.74 -1.4
4,786,150 - wzzB OKFHMN_24480 0.74 -0.1
4,786,150 - wzzB OKFHMN_24480 0.74 +0.2
4,786,189 + wzzB OKFHMN_24480 0.78 -1.5
4,786,258 + wzzB OKFHMN_24480 0.85 -1.7
4,786,269 - wzzB OKFHMN_24480 0.86 -0.5
4,786,481 - -0.4
4,786,628 - hisIE OKFHMN_24485 0.20 -2.9
4,786,628 - hisIE OKFHMN_24485 0.20 -3.4
4,786,701 - hisIE OKFHMN_24485 0.32 -3.3
4,786,891 + hisIE OKFHMN_24485 0.63 -2.4
4,786,895 - hisIE OKFHMN_24485 0.64 -3.6
4,787,009 + hisIE OKFHMN_24485 0.83 -5.0
4,787,514 - hisF OKFHMN_24490 0.52 -3.1
4,788,046 - hisA OKFHMN_24495 0.24 -5.4
4,788,046 - hisA OKFHMN_24495 0.24 -2.6

Or see this region's nucleotide sequence