Strain Fitness in Escherichia coli ECRC100 around OKFHMN_23455

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpsuK and psuG overlap by 13 nucleotidespsuG and psuT are separated by 93 nucleotides OKFHMN_23450: psuK - pseudouridine kinase, at 4,579,133 to 4,580,074 psuK OKFHMN_23455: psuG - pseudouridine-5'-phosphate glycosidase, at 4,580,062 to 4,581,000 psuG OKFHMN_23460: psuT - Putative pseudouridine transporter, at 4,581,094 to 4,582,344 psuT Position (kb) 4580 4581 4582Strain fitness (log2 ratio) -2 -1 0 1at 4579.082 kb on + strandat 4579.082 kb on + strandat 4579.082 kb on + strandat 4579.085 kb on - strandat 4579.132 kb on + strandat 4579.159 kb on + strandat 4579.561 kb on + strand, within psuKat 4579.678 kb on + strand, within psuKat 4579.821 kb on - strand, within psuKat 4580.039 kb on - strandat 4580.329 kb on - strand, within psuGat 4580.487 kb on + strand, within psuGat 4580.488 kb on - strand, within psuGat 4580.530 kb on - strand, within psuGat 4580.615 kb on + strand, within psuGat 4580.631 kb on + strand, within psuGat 4580.802 kb on + strand, within psuGat 4580.802 kb on + strand, within psuGat 4581.131 kb on + strandat 4581.235 kb on + strand, within psuTat 4581.285 kb on + strand, within psuTat 4581.442 kb on + strand, within psuTat 4581.443 kb on - strand, within psuTat 4581.694 kb on + strand, within psuT

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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4,579,082 + -1.2
4,579,082 + +0.3
4,579,082 + +0.7
4,579,085 - -1.2
4,579,132 + +0.8
4,579,159 + -0.0
4,579,561 + psuK OKFHMN_23450 0.45 -0.3
4,579,678 + psuK OKFHMN_23450 0.58 -0.2
4,579,821 - psuK OKFHMN_23450 0.73 +0.4
4,580,039 - +0.3
4,580,329 - psuG OKFHMN_23455 0.28 -2.4
4,580,487 + psuG OKFHMN_23455 0.45 -0.7
4,580,488 - psuG OKFHMN_23455 0.45 -0.7
4,580,530 - psuG OKFHMN_23455 0.50 -0.7
4,580,615 + psuG OKFHMN_23455 0.59 -0.6
4,580,631 + psuG OKFHMN_23455 0.61 +1.1
4,580,802 + psuG OKFHMN_23455 0.79 -1.1
4,580,802 + psuG OKFHMN_23455 0.79 -0.1
4,581,131 + +0.1
4,581,235 + psuT OKFHMN_23460 0.11 +0.7
4,581,285 + psuT OKFHMN_23460 0.15 +0.3
4,581,442 + psuT OKFHMN_23460 0.28 +0.8
4,581,443 - psuT OKFHMN_23460 0.28 +0.9
4,581,694 + psuT OKFHMN_23460 0.48 -2.6

Or see this region's nucleotide sequence