Strain Fitness in Escherichia coli ECRC100 around OKFHMN_20335

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntygcS and ygcS overlap by 4 nucleotidesygcS and ygcR overlap by 23 nucleotidesygcR and fixB overlap by 4 nucleotidesfixB and OKFHMN_20345 are separated by 15 nucleotides OKFHMN_20325: ygcS - Inner membrane metabolite transport protein YgcS, at 3,958,330 to 3,959,535 ygcS OKFHMN_20330: ygcS - Inner membrane metabolite transport protein YgcS, at 3,959,532 to 3,959,738 ygcS OKFHMN_20335: ygcR - Putative electron transfer flavoprotein subunit YgcR, at 3,959,716 to 3,960,495 ygcR OKFHMN_20340: fixB - electron transfer flavoprotein, at 3,960,492 to 3,961,061 fixB OKFHMN_20345: OKFHMN_20345 - Electron transfer flavoprotein subunit YgcQ, at 3,961,077 to 3,961,352 _20345 Position (kb) 3959 3960 3961Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3958.775 kb on - strand, within ygcSat 3958.781 kb on + strand, within ygcSat 3958.781 kb on + strand, within ygcSat 3958.862 kb on + strand, within ygcSat 3958.867 kb on + strand, within ygcSat 3958.867 kb on + strand, within ygcSat 3958.868 kb on - strand, within ygcSat 3958.868 kb on - strand, within ygcSat 3958.868 kb on - strand, within ygcSat 3958.966 kb on - strand, within ygcSat 3958.966 kb on - strand, within ygcSat 3959.104 kb on - strand, within ygcSat 3959.229 kb on + strand, within ygcSat 3959.229 kb on + strand, within ygcSat 3959.229 kb on + strand, within ygcSat 3959.268 kb on + strand, within ygcSat 3959.304 kb on - strand, within ygcSat 3959.375 kb on + strand, within ygcSat 3959.456 kb on + strandat 3959.608 kb on + strand, within ygcSat 3959.647 kb on + strand, within ygcSat 3959.647 kb on + strand, within ygcSat 3959.982 kb on - strand, within ygcRat 3960.210 kb on + strand, within ygcRat 3960.210 kb on + strand, within ygcRat 3960.458 kb on + strandat 3960.573 kb on - strand, within fixBat 3960.592 kb on + strand, within fixBat 3960.776 kb on - strand, within fixBat 3960.842 kb on - strand, within fixBat 3960.842 kb on - strand, within fixBat 3960.952 kb on + strand, within fixBat 3960.952 kb on + strand, within fixBat 3960.953 kb on - strand, within fixBat 3960.953 kb on - strand, within fixBat 3961.084 kb on - strandat 3961.169 kb on + strand, within OKFHMN_20345at 3961.202 kb on - strand, within OKFHMN_20345at 3961.220 kb on + strand, within OKFHMN_20345at 3961.241 kb on + strand, within OKFHMN_20345at 3961.398 kb on + strandat 3961.399 kb on - strandat 3961.407 kb on + strandat 3961.417 kb on + strandat 3961.461 kb on + strandat 3961.487 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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3,958,775 - ygcS OKFHMN_20325 0.37 -3.1
3,958,781 + ygcS OKFHMN_20325 0.37 +0.0
3,958,781 + ygcS OKFHMN_20325 0.37 -0.2
3,958,862 + ygcS OKFHMN_20325 0.44 +0.6
3,958,867 + ygcS OKFHMN_20325 0.45 +0.3
3,958,867 + ygcS OKFHMN_20325 0.45 +0.1
3,958,868 - ygcS OKFHMN_20325 0.45 -1.0
3,958,868 - ygcS OKFHMN_20325 0.45 +0.6
3,958,868 - ygcS OKFHMN_20325 0.45 +0.0
3,958,966 - ygcS OKFHMN_20325 0.53 -0.4
3,958,966 - ygcS OKFHMN_20325 0.53 +0.5
3,959,104 - ygcS OKFHMN_20325 0.64 +0.2
3,959,229 + ygcS OKFHMN_20325 0.75 +2.3
3,959,229 + ygcS OKFHMN_20325 0.75 +0.5
3,959,229 + ygcS OKFHMN_20325 0.75 -0.4
3,959,268 + ygcS OKFHMN_20325 0.78 +0.2
3,959,304 - ygcS OKFHMN_20325 0.81 -0.1
3,959,375 + ygcS OKFHMN_20325 0.87 -0.1
3,959,456 + +0.1
3,959,608 + ygcS OKFHMN_20330 0.37 -1.3
3,959,647 + ygcS OKFHMN_20330 0.56 +0.7
3,959,647 + ygcS OKFHMN_20330 0.56 +0.6
3,959,982 - ygcR OKFHMN_20335 0.34 +0.5
3,960,210 + ygcR OKFHMN_20335 0.63 -2.7
3,960,210 + ygcR OKFHMN_20335 0.63 +0.2
3,960,458 + -0.1
3,960,573 - fixB OKFHMN_20340 0.14 -1.0
3,960,592 + fixB OKFHMN_20340 0.18 +0.2
3,960,776 - fixB OKFHMN_20340 0.50 +2.4
3,960,842 - fixB OKFHMN_20340 0.61 -0.4
3,960,842 - fixB OKFHMN_20340 0.61 +0.6
3,960,952 + fixB OKFHMN_20340 0.81 -0.4
3,960,952 + fixB OKFHMN_20340 0.81 +0.1
3,960,953 - fixB OKFHMN_20340 0.81 -0.1
3,960,953 - fixB OKFHMN_20340 0.81 +0.4
3,961,084 - +0.8
3,961,169 + OKFHMN_20345 0.33 +1.6
3,961,202 - OKFHMN_20345 0.45 -0.4
3,961,220 + OKFHMN_20345 0.52 +0.1
3,961,241 + OKFHMN_20345 0.59 -0.3
3,961,398 + -0.1
3,961,399 - +0.4
3,961,407 + -0.1
3,961,417 + +0.3
3,961,461 + +0.1
3,961,487 + -1.6

Or see this region's nucleotide sequence