Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tnpB and OKFHMN_04200 are separated by 81 nucleotides OKFHMN_04200 and OKFHMN_04205 are separated by 3 nucleotides OKFHMN_04205 and tnpB are separated by 30 nucleotides tnpB and OKFHMN_04215 overlap by 4 nucleotides
OKFHMN_04195: tnpB - IS66 family insertion sequence element accessory protein TnpB, at 672,541 to 672,882
tnpB
OKFHMN_04200: OKFHMN_04200 - IS66 family transposase, at 672,964 to 673,821
_04200
OKFHMN_04205: OKFHMN_04205 - IS66-like element IS682 family transposase, at 673,825 to 675,438
_04205
OKFHMN_04210: tnpB - IS66 family insertion sequence element accessory protein TnpB, at 675,469 to 675,819
tnpB
OKFHMN_04215: OKFHMN_04215 - putative transposase of insertion sequence IS682, at 675,816 to 676,241
_04215
Position (kb) 673
674
675
676 Strain fitness (log2 ratio) -2
-1
0
1
2 at 673.417 kb on + strand, within OKFHMN_04200 at 673.483 kb on - strand, within OKFHMN_04200 at 673.698 kb on + strand, within OKFHMN_04200 at 673.777 kb on + strand at 674.062 kb on - strand, within OKFHMN_04205 at 674.094 kb on - strand, within OKFHMN_04205 at 674.731 kb on + strand, within OKFHMN_04205 at 675.688 kb on - strand, within tnpB at 675.759 kb on + strand, within tnpB at 675.760 kb on - strand, within tnpB at 675.766 kb on - strand, within tnpB at 675.766 kb on - strand, within tnpB at 675.891 kb on - strand, within OKFHMN_04215 at 676.275 kb on + strand at 676.275 kb on + strand at 676.294 kb on + strand at 676.301 kb on + strand at 676.305 kb on + strand at 676.422 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 673,417 + OKFHMN_04200 0.53 +2.2 673,483 - OKFHMN_04200 0.60 -0.6 673,698 + OKFHMN_04200 0.86 +0.9 673,777 + +0.5 674,062 - OKFHMN_04205 0.15 +1.0 674,094 - OKFHMN_04205 0.17 +1.5 674,731 + OKFHMN_04205 0.56 -0.2 675,688 - tnpB OKFHMN_04210 0.62 +0.9 675,759 + tnpB OKFHMN_04210 0.83 -0.4 675,760 - tnpB OKFHMN_04210 0.83 +0.1 675,766 - tnpB OKFHMN_04210 0.85 -0.5 675,766 - tnpB OKFHMN_04210 0.85 -2.5 675,891 - OKFHMN_04215 0.18 +0.6 676,275 + +0.1 676,275 + -0.7 676,294 + +0.9 676,301 + +0.2 676,305 + -1.2 676,422 + -0.2
Or see this region's nucleotide sequence