Strain Fitness in Escherichia coli ECRC100 around OKFHMN_02560

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlapB and lapA are separated by 6 nucleotideslapA and pgpB are separated by 148 nucleotides OKFHMN_02555: lapB - lipopolysaccharide assembly protein LapB, at 402,481 to 403,650 lapB OKFHMN_02560: lapA - lipopolysaccharide assembly protein LapA, at 403,657 to 403,965 lapA OKFHMN_02565: pgpB - phosphatidylglycerophosphatase B, at 404,114 to 404,878 pgpB Position (kb) 403 404Strain fitness (log2 ratio) -1 0 1 2at 403.865 kb on - strand, within lapAat 403.969 kb on - strandat 404.028 kb on - strandat 404.234 kb on - strand, within pgpBat 404.237 kb on + strand, within pgpBat 404.238 kb on - strand, within pgpBat 404.896 kb on - strandat 404.910 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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403,865 - lapA OKFHMN_02560 0.67 +1.4
403,969 - +1.3
404,028 - -0.2
404,234 - pgpB OKFHMN_02565 0.16 +0.6
404,237 + pgpB OKFHMN_02565 0.16 +0.2
404,238 - pgpB OKFHMN_02565 0.16 -0.4
404,896 - +2.1
404,910 + -0.9

Or see this region's nucleotide sequence