Strain Fitness in Escherichia coli ECRC100 around OKFHMN_02455

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpuuP and OKFHMN_02455 are separated by 133 nucleotidesOKFHMN_02455 and sapA are separated by 312 nucleotides OKFHMN_02450: puuP - putrescine/proton symporter PuuP, at 377,796 to 379,181 puuP OKFHMN_02455: OKFHMN_02455 - DUF2543 domain-containing protein, at 379,315 to 379,560 _02455 OKFHMN_02460: sapA - peptide ABC transporter substrate-binding protein SapA, at 379,873 to 381,516 sapA Position (kb) 379 380Strain fitness (log2 ratio) -3 -2 -1 0 1at 378.318 kb on - strand, within puuPat 378.318 kb on - strand, within puuPat 378.700 kb on + strand, within puuPat 379.202 kb on + strandat 379.708 kb on + strandat 379.710 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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378,318 - puuP OKFHMN_02450 0.38 -0.2
378,318 - puuP OKFHMN_02450 0.38 +0.3
378,700 + puuP OKFHMN_02450 0.65 -2.9
379,202 + +0.5
379,708 + +0.1
379,710 + +0.0

Or see this region's nucleotide sequence