Strain Fitness in Escherichia coli ECRC100 around OKFHMN_02420

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpspF and puuE are separated by 130 nucleotidespuuE and puuB are separated by 37 nucleotides OKFHMN_02415: pspF - phage shock protein operon transcriptional activator, at 369,185 to 370,162 pspF OKFHMN_02420: puuE - 4-aminobutyrate transaminase, at 370,293 to 371,558 puuE OKFHMN_02425: puuB - gamma-glutamylputrescine oxidase, at 371,596 to 372,876 puuB Position (kb) 370 371 372Strain fitness (log2 ratio) -2 -1 0 1at 369.336 kb on - strand, within pspFat 369.373 kb on + strand, within pspFat 369.618 kb on + strand, within pspFat 369.619 kb on - strand, within pspFat 369.702 kb on + strand, within pspFat 369.742 kb on + strand, within pspFat 370.020 kb on + strand, within pspFat 370.216 kb on - strandat 370.216 kb on - strandat 370.220 kb on - strandat 370.220 kb on - strandat 370.226 kb on + strandat 370.258 kb on - strandat 370.268 kb on - strandat 370.710 kb on - strand, within puuEat 370.988 kb on + strand, within puuEat 371.050 kb on + strand, within puuEat 371.055 kb on + strand, within puuEat 371.134 kb on + strand, within puuEat 371.805 kb on - strand, within puuBat 372.167 kb on + strand, within puuBat 372.167 kb on + strand, within puuBat 372.192 kb on - strand, within puuBat 372.220 kb on - strand, within puuB

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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369,336 - pspF OKFHMN_02415 0.15 +0.6
369,373 + pspF OKFHMN_02415 0.19 -1.3
369,618 + pspF OKFHMN_02415 0.44 +0.1
369,619 - pspF OKFHMN_02415 0.44 +0.9
369,702 + pspF OKFHMN_02415 0.53 -0.8
369,742 + pspF OKFHMN_02415 0.57 -1.1
370,020 + pspF OKFHMN_02415 0.85 -0.2
370,216 - +0.2
370,216 - -0.3
370,220 - -2.3
370,220 - +0.8
370,226 + +0.2
370,258 - -0.5
370,268 - +0.2
370,710 - puuE OKFHMN_02420 0.33 +0.4
370,988 + puuE OKFHMN_02420 0.55 -1.2
371,050 + puuE OKFHMN_02420 0.60 -2.0
371,055 + puuE OKFHMN_02420 0.60 -1.7
371,134 + puuE OKFHMN_02420 0.66 +0.9
371,805 - puuB OKFHMN_02425 0.16 +0.5
372,167 + puuB OKFHMN_02425 0.45 +0.1
372,167 + puuB OKFHMN_02425 0.45 +0.4
372,192 - puuB OKFHMN_02425 0.47 -0.5
372,220 - puuB OKFHMN_02425 0.49 +0.2

Or see this region's nucleotide sequence