Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yncD and mcbR are separated by 35 nucleotides mcbR and curA are separated by 197 nucleotides curA and mddA are separated by 180 nucleotides mddA and OKFHMN_01540 overlap by 4 nucleotides
OKFHMN_01520: yncD - putative TonB-dependent receptor YncD, at 212,238 to 214,340
yncD
OKFHMN_01525: mcbR - colanic acid/biofilm transcriptional regulator McbR, at 214,376 to 215,041
mcbR
OKFHMN_01530: curA - NADPH-dependent curcumin/dihydrocurcumin reductase, at 215,239 to 216,276
curA
OKFHMN_01535: mddA - L-methionine sulfoximine/L-methionine sulfone acetyltransferase, at 216,457 to 216,975
mddA
OKFHMN_01540: OKFHMN_01540 - Putative inner membrane exporter, YdcZ, at 216,972 to 217,421
_01540
Position (kb) 215
216
217 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 214.338 kb on + strand at 214.342 kb on + strand at 215.175 kb on - strand at 215.560 kb on - strand, within curA at 215.562 kb on + strand, within curA at 215.563 kb on - strand, within curA at 215.963 kb on + strand, within curA at 216.087 kb on - strand, within curA at 216.106 kb on - strand, within curA at 216.315 kb on + strand at 216.527 kb on - strand, within mddA at 216.529 kb on - strand, within mddA at 216.599 kb on + strand, within mddA at 216.750 kb on + strand, within mddA at 217.068 kb on - strand, within OKFHMN_01540 at 217.194 kb on - strand, within OKFHMN_01540
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 214,338 + +1.3 214,342 + +1.6 215,175 - -0.4 215,560 - curA OKFHMN_01530 0.31 +0.8 215,562 + curA OKFHMN_01530 0.31 -0.2 215,563 - curA OKFHMN_01530 0.31 +0.8 215,963 + curA OKFHMN_01530 0.70 -1.3 216,087 - curA OKFHMN_01530 0.82 +1.2 216,106 - curA OKFHMN_01530 0.84 +0.9 216,315 + +0.1 216,527 - mddA OKFHMN_01535 0.13 +0.1 216,529 - mddA OKFHMN_01535 0.14 -0.5 216,599 + mddA OKFHMN_01535 0.27 -1.1 216,750 + mddA OKFHMN_01535 0.56 -3.9 217,068 - OKFHMN_01540 0.21 -0.5 217,194 - OKFHMN_01540 0.49 -3.9
Or see this region's nucleotide sequence