Strain Fitness in Escherichia coli ECRC100 around OKFHMN_01435

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnarH and narW overlap by 1 nucleotidesnarW and narI overlap by 4 nucleotidesnarI and yddE are separated by 78 nucleotides OKFHMN_01425: narH - nitrate reductase subunit beta, at 192,968 to 194,512 narH OKFHMN_01430: narW - nitrate reductase molybdenum cofactor assembly chaperone, at 194,512 to 195,207 narW OKFHMN_01435: narI - respiratory nitrate reductase subunit gamma, at 195,204 to 195,884 narI OKFHMN_01440: yddE - PhzF family isomerase, at 195,963 to 196,856 yddE Position (kb) 195 196Strain fitness (log2 ratio) -3 -2 -1 0 1at 194.266 kb on - strand, within narHat 194.858 kb on + strand, within narWat 195.303 kb on + strand, within narIat 195.629 kb on - strand, within narIat 195.937 kb on - strandat 196.207 kb on + strand, within yddEat 196.672 kb on + strand, within yddEat 196.672 kb on + strand, within yddE

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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194,266 - narH OKFHMN_01425 0.84 -0.6
194,858 + narW OKFHMN_01430 0.50 -0.6
195,303 + narI OKFHMN_01435 0.15 -3.6
195,629 - narI OKFHMN_01435 0.62 +0.3
195,937 - +1.1
196,207 + yddE OKFHMN_01440 0.27 +0.0
196,672 + yddE OKFHMN_01440 0.79 +0.2
196,672 + yddE OKFHMN_01440 0.79 -1.2

Or see this region's nucleotide sequence