Strain Fitness in Escherichia coli ECRC100 around OKFHMN_01110

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyneJ and sad are separated by 100 nucleotidessad and glsB are separated by 63 nucleotidesglsB and yneG overlap by 1 nucleotides OKFHMN_01105: yneJ - Uncharacterized HTH-type transcriptional regulator YneJ, at 108,511 to 109,392 yneJ OKFHMN_01110: sad - succinate-semialdehyde dehydrogenase, at 109,493 to 110,881 sad OKFHMN_01115: glsB - glutaminase B, at 110,945 to 111,871 glsB OKFHMN_01120: yneG - Uncharacterized protein YneG, at 111,871 to 112,230 yneG Position (kb) 109 110 111Strain fitness (log2 ratio) -2 -1 0 1 2at 109.258 kb on + strand, within yneJat 109.477 kb on + strandat 109.646 kb on + strand, within sadat 109.855 kb on + strand, within sadat 109.856 kb on - strand, within sadat 109.891 kb on + strand, within sadat 109.962 kb on - strand, within sadat 109.965 kb on + strand, within sadat 109.969 kb on + strand, within sadat 110.046 kb on + strand, within sadat 110.048 kb on + strand, within sadat 110.048 kb on + strand, within sadat 111.355 kb on + strand, within glsB

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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109,258 + yneJ OKFHMN_01105 0.85 +2.0
109,477 + -2.4
109,646 + sad OKFHMN_01110 0.11 -1.0
109,855 + sad OKFHMN_01110 0.26 +0.9
109,856 - sad OKFHMN_01110 0.26 -0.5
109,891 + sad OKFHMN_01110 0.29 +0.6
109,962 - sad OKFHMN_01110 0.34 +0.2
109,965 + sad OKFHMN_01110 0.34 -0.5
109,969 + sad OKFHMN_01110 0.34 +0.4
110,046 + sad OKFHMN_01110 0.40 +0.2
110,048 + sad OKFHMN_01110 0.40 +0.5
110,048 + sad OKFHMN_01110 0.40 -0.6
111,355 + glsB OKFHMN_01115 0.44 -1.0

Or see this region's nucleotide sequence