Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt adrA and psiF are separated by 101 nucleotides psiF and phoA are separated by 118 nucleotides phoA and iraP are separated by 100 nucleotides iraP and ddlA are separated by 462 nucleotides
BNILDI_22955: adrA - diguanylate cyclase AdrA, at 4,620,110 to 4,621,225
adrA
BNILDI_22960: psiF - phosphate starvation-inducible protein PsiF, at 4,621,327 to 4,621,647
psiF
BNILDI_22965: phoA - alkaline phosphatase, at 4,621,766 to 4,623,181
phoA
BNILDI_22970: iraP - anti-adapter protein IraP, at 4,623,282 to 4,623,542
iraP
BNILDI_22975: ddlA - D-alanine--D-alanine ligase, at 4,624,005 to 4,625,099
ddlA
Position (kb) 4621
4622
4623
4624 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4620.839 kb on - strand, within adrA at 4621.155 kb on - strand at 4621.155 kb on - strand at 4621.247 kb on + strand at 4621.247 kb on + strand at 4621.249 kb on + strand at 4621.249 kb on + strand at 4621.274 kb on - strand at 4621.300 kb on + strand at 4621.319 kb on - strand at 4621.326 kb on - strand at 4621.357 kb on - strand at 4621.357 kb on - strand at 4621.363 kb on - strand, within psiF at 4621.551 kb on + strand, within psiF at 4621.552 kb on - strand, within psiF at 4621.552 kb on - strand, within psiF at 4621.703 kb on + strand at 4621.703 kb on + strand at 4621.704 kb on - strand at 4621.717 kb on - strand at 4621.797 kb on + strand at 4621.797 kb on + strand at 4621.798 kb on - strand at 4621.880 kb on - strand at 4622.070 kb on - strand, within phoA at 4622.326 kb on + strand, within phoA at 4622.823 kb on + strand, within phoA at 4622.866 kb on - strand, within phoA at 4622.929 kb on + strand, within phoA at 4622.929 kb on + strand, within phoA at 4622.929 kb on + strand, within phoA at 4622.929 kb on + strand, within phoA at 4622.930 kb on - strand, within phoA at 4623.004 kb on + strand, within phoA at 4623.197 kb on + strand at 4623.232 kb on + strand at 4623.373 kb on + strand, within iraP at 4623.373 kb on + strand, within iraP at 4623.387 kb on + strand, within iraP at 4623.425 kb on + strand, within iraP at 4623.426 kb on - strand, within iraP at 4623.426 kb on - strand, within iraP at 4623.548 kb on + strand at 4623.549 kb on - strand at 4623.549 kb on - strand at 4623.582 kb on + strand at 4623.616 kb on - strand at 4623.649 kb on - strand at 4623.711 kb on - strand at 4623.810 kb on + strand at 4623.810 kb on + strand at 4623.892 kb on + strand at 4623.965 kb on + strand at 4623.966 kb on - strand at 4623.966 kb on - strand at 4623.970 kb on + strand at 4623.971 kb on - strand at 4624.001 kb on + strand at 4624.021 kb on + strand at 4624.101 kb on - strand at 4624.181 kb on - strand, within ddlA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,620,839 - adrA BNILDI_22955 0.65 -1.0 4,621,155 - +0.1 4,621,155 - -0.5 4,621,247 + +0.2 4,621,247 + +0.2 4,621,249 + -0.0 4,621,249 + +0.1 4,621,274 - +0.7 4,621,300 + -0.6 4,621,319 - -0.2 4,621,326 - -0.8 4,621,357 - -0.0 4,621,357 - -2.7 4,621,363 - psiF BNILDI_22960 0.11 -0.2 4,621,551 + psiF BNILDI_22960 0.70 -1.4 4,621,552 - psiF BNILDI_22960 0.70 +0.1 4,621,552 - psiF BNILDI_22960 0.70 +0.0 4,621,703 + +0.1 4,621,703 + +0.0 4,621,704 - +0.0 4,621,717 - +0.1 4,621,797 + -0.0 4,621,797 + -2.8 4,621,798 - -0.5 4,621,880 - +0.1 4,622,070 - phoA BNILDI_22965 0.21 +0.4 4,622,326 + phoA BNILDI_22965 0.40 -0.8 4,622,823 + phoA BNILDI_22965 0.75 +0.3 4,622,866 - phoA BNILDI_22965 0.78 +1.0 4,622,929 + phoA BNILDI_22965 0.82 +0.0 4,622,929 + phoA BNILDI_22965 0.82 -1.2 4,622,929 + phoA BNILDI_22965 0.82 -0.2 4,622,929 + phoA BNILDI_22965 0.82 +1.0 4,622,930 - phoA BNILDI_22965 0.82 +0.9 4,623,004 + phoA BNILDI_22965 0.87 -0.0 4,623,197 + +0.2 4,623,232 + +0.7 4,623,373 + iraP BNILDI_22970 0.35 -0.0 4,623,373 + iraP BNILDI_22970 0.35 -0.4 4,623,387 + iraP BNILDI_22970 0.40 -0.7 4,623,425 + iraP BNILDI_22970 0.55 -0.2 4,623,426 - iraP BNILDI_22970 0.55 -0.4 4,623,426 - iraP BNILDI_22970 0.55 +0.3 4,623,548 + -0.3 4,623,549 - -0.0 4,623,549 - -0.2 4,623,582 + -0.2 4,623,616 - +0.8 4,623,649 - -0.3 4,623,711 - +0.7 4,623,810 + -0.3 4,623,810 + +0.2 4,623,892 + -0.0 4,623,965 + -1.1 4,623,966 - -0.3 4,623,966 - +0.3 4,623,970 + -0.0 4,623,971 - +0.6 4,624,001 + -2.8 4,624,021 + -0.5 4,624,101 - -0.1 4,624,181 - ddlA BNILDI_22975 0.16 +1.0
Or see this region's nucleotide sequence