Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mak and rdgC are separated by 58 nucleotides rdgC and BNILDI_22920 are separated by 77 nucleotides BNILDI_22920 and ppnP are separated by 260 nucleotides
BNILDI_22910: mak - fructokinase, at 4,613,822 to 4,614,730
mak
BNILDI_22915: rdgC - recombination-associated protein RdgC, at 4,614,789 to 4,615,766
rdgC
BNILDI_22920: BNILDI_22920 - hypothetical protein, at 4,615,844 to 4,616,206
_22920
BNILDI_22925: ppnP - pyrimidine/purine nucleoside phosphorylase, at 4,616,467 to 4,616,751
ppnP
Position (kb)
4614
4615
4616 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4613.952 kb on - strand, within mak at 4613.960 kb on + strand, within mak at 4613.961 kb on - strand, within mak at 4613.961 kb on - strand, within mak at 4614.357 kb on - strand, within mak at 4614.386 kb on + strand, within mak at 4614.387 kb on - strand, within mak at 4614.387 kb on - strand, within mak at 4614.657 kb on + strand at 4614.722 kb on + strand at 4614.723 kb on - strand at 4614.723 kb on - strand at 4614.723 kb on - strand at 4614.765 kb on + strand at 4614.825 kb on - strand at 4614.852 kb on - strand at 4614.852 kb on - strand at 4614.852 kb on - strand at 4615.094 kb on + strand, within rdgC at 4615.145 kb on + strand, within rdgC at 4615.223 kb on + strand, within rdgC at 4615.320 kb on - strand, within rdgC at 4615.345 kb on + strand, within rdgC at 4615.544 kb on + strand, within rdgC at 4615.744 kb on + strand at 4615.764 kb on + strand at 4615.765 kb on - strand at 4615.841 kb on + strand at 4615.841 kb on + strand at 4615.841 kb on + strand at 4615.842 kb on - strand at 4615.842 kb on - strand at 4615.842 kb on - strand at 4615.882 kb on + strand, within BNILDI_22920 at 4615.883 kb on - strand, within BNILDI_22920 at 4616.018 kb on - strand, within BNILDI_22920 at 4616.019 kb on + strand, within BNILDI_22920 at 4616.117 kb on - strand, within BNILDI_22920 at 4616.149 kb on + strand, within BNILDI_22920 at 4616.166 kb on + strand, within BNILDI_22920 at 4616.175 kb on - strand at 4616.253 kb on - strand at 4616.316 kb on + strand at 4616.340 kb on + strand at 4616.341 kb on - strand at 4616.360 kb on - strand at 4616.469 kb on - strand at 4616.538 kb on + strand, within ppnP at 4616.558 kb on + strand, within ppnP at 4616.558 kb on + strand, within ppnP at 4616.559 kb on - strand, within ppnP at 4616.588 kb on - strand, within ppnP
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,613,952 - mak BNILDI_22910 0.14 +1.5 4,613,960 + mak BNILDI_22910 0.15 -0.1 4,613,961 - mak BNILDI_22910 0.15 -0.3 4,613,961 - mak BNILDI_22910 0.15 -1.4 4,614,357 - mak BNILDI_22910 0.59 -0.7 4,614,386 + mak BNILDI_22910 0.62 -0.2 4,614,387 - mak BNILDI_22910 0.62 +1.5 4,614,387 - mak BNILDI_22910 0.62 +0.9 4,614,657 + +0.8 4,614,722 + -0.8 4,614,723 - +0.6 4,614,723 - -0.0 4,614,723 - +0.7 4,614,765 + +0.2 4,614,825 - -1.3 4,614,852 - +0.7 4,614,852 - +1.2 4,614,852 - -0.0 4,615,094 + rdgC BNILDI_22915 0.31 +0.1 4,615,145 + rdgC BNILDI_22915 0.36 +0.2 4,615,223 + rdgC BNILDI_22915 0.44 -1.3 4,615,320 - rdgC BNILDI_22915 0.54 +0.2 4,615,345 + rdgC BNILDI_22915 0.57 +0.7 4,615,544 + rdgC BNILDI_22915 0.77 +0.1 4,615,744 + -0.8 4,615,764 + +0.0 4,615,765 - +1.0 4,615,841 + -1.1 4,615,841 + +0.9 4,615,841 + +0.9 4,615,842 - -0.0 4,615,842 - +0.0 4,615,842 - +0.4 4,615,882 + BNILDI_22920 0.10 +0.2 4,615,883 - BNILDI_22920 0.11 +0.7 4,616,018 - BNILDI_22920 0.48 -0.3 4,616,019 + BNILDI_22920 0.48 +0.0 4,616,117 - BNILDI_22920 0.75 -2.4 4,616,149 + BNILDI_22920 0.84 -2.0 4,616,166 + BNILDI_22920 0.89 -0.1 4,616,175 - -0.5 4,616,253 - +2.3 4,616,316 + -0.0 4,616,340 + -1.7 4,616,341 - -0.5 4,616,360 - -0.8 4,616,469 - -2.2 4,616,538 + ppnP BNILDI_22925 0.25 +0.1 4,616,558 + ppnP BNILDI_22925 0.32 +0.0 4,616,558 + ppnP BNILDI_22925 0.32 +0.4 4,616,559 - ppnP BNILDI_22925 0.32 +0.4 4,616,588 - ppnP BNILDI_22925 0.42 +0.3
Or see this region's nucleotide sequence