Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phoR and phoB are separated by 57 nucleotides phoB and sbcD are separated by 189 nucleotides
BNILDI_22885: phoR - phosphate regulon sensor histidine kinase PhoR, at 4,605,795 to 4,607,090
phoR
BNILDI_22890: phoB - phosphate response regulator transcription factor PhoB, at 4,607,148 to 4,607,837
phoB
BNILDI_22895: sbcD - exonuclease subunit SbcD, at 4,608,027 to 4,609,229
sbcD
Position (kb)
4607
4608 Strain fitness (log2 ratio)
-2
-1
0
1 at 4606.176 kb on + strand, within phoR at 4606.188 kb on - strand, within phoR at 4606.188 kb on - strand, within phoR at 4606.293 kb on + strand, within phoR at 4606.446 kb on + strand, within phoR at 4606.447 kb on - strand, within phoR at 4606.544 kb on + strand, within phoR at 4606.627 kb on + strand, within phoR at 4606.627 kb on + strand, within phoR at 4606.627 kb on + strand, within phoR at 4606.832 kb on + strand, within phoR at 4606.863 kb on + strand, within phoR at 4606.863 kb on + strand, within phoR at 4606.864 kb on - strand, within phoR at 4606.907 kb on + strand, within phoR at 4606.907 kb on + strand, within phoR at 4606.907 kb on + strand, within phoR at 4606.908 kb on - strand, within phoR at 4607.046 kb on + strand at 4607.046 kb on + strand at 4607.047 kb on - strand at 4607.117 kb on - strand at 4607.272 kb on + strand, within phoB at 4607.273 kb on - strand, within phoB at 4607.273 kb on - strand, within phoB at 4607.273 kb on - strand, within phoB at 4607.273 kb on - strand, within phoB at 4607.280 kb on - strand, within phoB at 4607.317 kb on + strand, within phoB at 4607.318 kb on - strand, within phoB at 4607.427 kb on + strand, within phoB at 4607.531 kb on + strand, within phoB at 4607.533 kb on + strand, within phoB at 4607.534 kb on - strand, within phoB at 4607.534 kb on - strand, within phoB at 4607.534 kb on - strand, within phoB at 4607.670 kb on + strand, within phoB at 4607.670 kb on + strand, within phoB at 4607.691 kb on + strand, within phoB at 4607.734 kb on + strand, within phoB at 4607.825 kb on + strand at 4607.852 kb on + strand at 4607.852 kb on + strand at 4607.853 kb on - strand at 4607.853 kb on - strand at 4607.855 kb on + strand at 4607.856 kb on - strand at 4607.860 kb on - strand at 4607.860 kb on - strand at 4607.932 kb on - strand at 4607.932 kb on - strand at 4608.072 kb on + strand at 4608.073 kb on - strand at 4608.077 kb on + strand at 4608.156 kb on - strand, within sbcD at 4608.213 kb on + strand, within sbcD at 4608.213 kb on + strand, within sbcD at 4608.213 kb on + strand, within sbcD at 4608.262 kb on + strand, within sbcD at 4608.581 kb on - strand, within sbcD at 4608.693 kb on - strand, within sbcD at 4608.782 kb on + strand, within sbcD at 4608.783 kb on - strand, within sbcD at 4608.783 kb on - strand, within sbcD at 4608.786 kb on + strand, within sbcD at 4608.786 kb on + strand, within sbcD at 4608.786 kb on + strand, within sbcD at 4608.787 kb on - strand, within sbcD at 4608.787 kb on - strand, within sbcD
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,606,176 + phoR BNILDI_22885 0.29 +0.2 4,606,188 - phoR BNILDI_22885 0.30 -0.4 4,606,188 - phoR BNILDI_22885 0.30 -0.0 4,606,293 + phoR BNILDI_22885 0.38 -0.5 4,606,446 + phoR BNILDI_22885 0.50 -1.7 4,606,447 - phoR BNILDI_22885 0.50 -0.4 4,606,544 + phoR BNILDI_22885 0.58 +0.6 4,606,627 + phoR BNILDI_22885 0.64 -0.1 4,606,627 + phoR BNILDI_22885 0.64 -0.1 4,606,627 + phoR BNILDI_22885 0.64 -0.5 4,606,832 + phoR BNILDI_22885 0.80 -0.5 4,606,863 + phoR BNILDI_22885 0.82 -0.5 4,606,863 + phoR BNILDI_22885 0.82 -1.3 4,606,864 - phoR BNILDI_22885 0.82 +0.2 4,606,907 + phoR BNILDI_22885 0.86 -1.2 4,606,907 + phoR BNILDI_22885 0.86 +0.6 4,606,907 + phoR BNILDI_22885 0.86 +0.8 4,606,908 - phoR BNILDI_22885 0.86 +0.0 4,607,046 + +0.0 4,607,046 + -0.4 4,607,047 - +0.2 4,607,117 - -2.0 4,607,272 + phoB BNILDI_22890 0.18 +0.0 4,607,273 - phoB BNILDI_22890 0.18 +1.4 4,607,273 - phoB BNILDI_22890 0.18 +0.6 4,607,273 - phoB BNILDI_22890 0.18 +1.1 4,607,273 - phoB BNILDI_22890 0.18 -0.1 4,607,280 - phoB BNILDI_22890 0.19 -0.2 4,607,317 + phoB BNILDI_22890 0.24 -0.5 4,607,318 - phoB BNILDI_22890 0.25 +0.4 4,607,427 + phoB BNILDI_22890 0.40 -0.4 4,607,531 + phoB BNILDI_22890 0.56 -0.3 4,607,533 + phoB BNILDI_22890 0.56 +0.5 4,607,534 - phoB BNILDI_22890 0.56 -0.7 4,607,534 - phoB BNILDI_22890 0.56 +0.1 4,607,534 - phoB BNILDI_22890 0.56 +1.7 4,607,670 + phoB BNILDI_22890 0.76 -0.3 4,607,670 + phoB BNILDI_22890 0.76 +0.4 4,607,691 + phoB BNILDI_22890 0.79 +0.3 4,607,734 + phoB BNILDI_22890 0.85 -0.2 4,607,825 + +0.1 4,607,852 + -0.1 4,607,852 + +0.8 4,607,853 - -0.0 4,607,853 - +0.1 4,607,855 + +0.4 4,607,856 - +0.3 4,607,860 - +0.2 4,607,860 - -0.4 4,607,932 - -0.5 4,607,932 - -1.2 4,608,072 + -0.3 4,608,073 - -0.1 4,608,077 + +1.0 4,608,156 - sbcD BNILDI_22895 0.11 +0.2 4,608,213 + sbcD BNILDI_22895 0.15 -0.1 4,608,213 + sbcD BNILDI_22895 0.15 -1.4 4,608,213 + sbcD BNILDI_22895 0.15 -0.0 4,608,262 + sbcD BNILDI_22895 0.20 -0.4 4,608,581 - sbcD BNILDI_22895 0.46 -0.8 4,608,693 - sbcD BNILDI_22895 0.55 -1.3 4,608,782 + sbcD BNILDI_22895 0.63 +0.1 4,608,783 - sbcD BNILDI_22895 0.63 +0.8 4,608,783 - sbcD BNILDI_22895 0.63 +1.5 4,608,786 + sbcD BNILDI_22895 0.63 +1.2 4,608,786 + sbcD BNILDI_22895 0.63 -0.5 4,608,786 + sbcD BNILDI_22895 0.63 -0.0 4,608,787 - sbcD BNILDI_22895 0.63 -0.0 4,608,787 - sbcD BNILDI_22895 0.63 -0.3
Or see this region's nucleotide sequence