Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lon_P403L and clpX are separated by 187 nucleotides clpX and clpP are separated by 125 nucleotides clpP and tig are separated by 245 nucleotides
BNILDI_22670: lon_P403L - Lon protease, at 4,561,464 to 4,563,818
_P403L
BNILDI_22680: clpX - ATP-dependent protease ATP-binding subunit ClpX, at 4,564,006 to 4,565,280
clpX
BNILDI_22685: clpP - ATP-dependent Clp endopeptidase proteolytic subunit ClpP, at 4,565,406 to 4,566,029
clpP
BNILDI_22690: tig - trigger factor, at 4,566,275 to 4,567,573
tig
Position (kb)
4564
4565
4566 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4563.136 kb on + strand, within lon_P403L at 4563.302 kb on + strand, within lon_P403L at 4563.883 kb on + strand at 4563.894 kb on - strand at 4563.894 kb on - strand at 4563.894 kb on - strand at 4563.902 kb on - strand at 4563.903 kb on + strand at 4563.985 kb on + strand at 4563.985 kb on + strand at 4564.044 kb on - strand at 4564.076 kb on - strand at 4564.128 kb on - strand at 4564.128 kb on - strand at 4564.154 kb on - strand, within clpX at 4564.770 kb on - strand, within clpX at 4564.770 kb on - strand, within clpX at 4565.148 kb on + strand, within clpX at 4565.149 kb on - strand, within clpX at 4565.153 kb on - strand at 4565.175 kb on + strand at 4565.177 kb on + strand at 4565.178 kb on - strand at 4565.178 kb on - strand at 4565.380 kb on - strand at 4565.380 kb on - strand at 4565.411 kb on + strand at 4565.412 kb on - strand at 4565.412 kb on - strand at 4565.759 kb on - strand, within clpP at 4565.762 kb on + strand, within clpP at 4565.762 kb on + strand, within clpP at 4565.763 kb on - strand, within clpP at 4565.799 kb on - strand, within clpP at 4565.799 kb on - strand, within clpP at 4565.809 kb on + strand, within clpP at 4565.810 kb on - strand, within clpP at 4565.810 kb on - strand, within clpP at 4565.813 kb on + strand, within clpP at 4565.919 kb on + strand, within clpP at 4565.929 kb on + strand, within clpP at 4566.005 kb on + strand at 4566.022 kb on + strand at 4566.075 kb on - strand at 4566.075 kb on - strand at 4566.075 kb on - strand at 4566.080 kb on + strand at 4566.098 kb on + strand at 4566.140 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,563,136 + lon_P403L BNILDI_22670 0.71 -3.1 4,563,302 + lon_P403L BNILDI_22670 0.78 -1.4 4,563,883 + -3.0 4,563,894 - -1.2 4,563,894 - +0.2 4,563,894 - +0.5 4,563,902 - -0.9 4,563,903 + -2.0 4,563,985 + -0.3 4,563,985 + -0.3 4,564,044 - -0.6 4,564,076 - -0.8 4,564,128 - +1.9 4,564,128 - -0.7 4,564,154 - clpX BNILDI_22680 0.12 -2.3 4,564,770 - clpX BNILDI_22680 0.60 +1.2 4,564,770 - clpX BNILDI_22680 0.60 -0.9 4,565,148 + clpX BNILDI_22680 0.90 -3.0 4,565,149 - clpX BNILDI_22680 0.90 -0.7 4,565,153 - -1.7 4,565,175 + -0.9 4,565,177 + -2.1 4,565,178 - -2.8 4,565,178 - -2.2 4,565,380 - +0.5 4,565,380 - -0.1 4,565,411 + -1.2 4,565,412 - -1.5 4,565,412 - -0.7 4,565,759 - clpP BNILDI_22685 0.57 -1.7 4,565,762 + clpP BNILDI_22685 0.57 +0.9 4,565,762 + clpP BNILDI_22685 0.57 -0.1 4,565,763 - clpP BNILDI_22685 0.57 -3.0 4,565,799 - clpP BNILDI_22685 0.63 +0.0 4,565,799 - clpP BNILDI_22685 0.63 -2.0 4,565,809 + clpP BNILDI_22685 0.65 +0.7 4,565,810 - clpP BNILDI_22685 0.65 -1.2 4,565,810 - clpP BNILDI_22685 0.65 -1.5 4,565,813 + clpP BNILDI_22685 0.65 -2.6 4,565,919 + clpP BNILDI_22685 0.82 -2.7 4,565,929 + clpP BNILDI_22685 0.84 -2.5 4,566,005 + -2.9 4,566,022 + -2.8 4,566,075 - +0.4 4,566,075 - +0.3 4,566,075 - +0.1 4,566,080 + -1.1 4,566,098 + -0.9 4,566,140 + -0.0
Or see this region's nucleotide sequence