Strain Fitness in Escherichia coli ECRC62 around BNILDI_22680

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlon_P403L and clpX are separated by 187 nucleotidesclpX and clpP are separated by 125 nucleotidesclpP and tig are separated by 245 nucleotides BNILDI_22670: lon_P403L - Lon protease, at 4,561,464 to 4,563,818 _P403L BNILDI_22680: clpX - ATP-dependent protease ATP-binding subunit ClpX, at 4,564,006 to 4,565,280 clpX BNILDI_22685: clpP - ATP-dependent Clp endopeptidase proteolytic subunit ClpP, at 4,565,406 to 4,566,029 clpP BNILDI_22690: tig - trigger factor, at 4,566,275 to 4,567,573 tig Position (kb) 4564 4565 4566Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4563.136 kb on + strand, within lon_P403Lat 4563.302 kb on + strand, within lon_P403Lat 4563.883 kb on + strandat 4563.894 kb on - strandat 4563.894 kb on - strandat 4563.894 kb on - strandat 4563.902 kb on - strandat 4563.903 kb on + strandat 4563.985 kb on + strandat 4563.985 kb on + strandat 4564.044 kb on - strandat 4564.076 kb on - strandat 4564.128 kb on - strandat 4564.128 kb on - strandat 4564.154 kb on - strand, within clpXat 4564.770 kb on - strand, within clpXat 4564.770 kb on - strand, within clpXat 4565.148 kb on + strand, within clpXat 4565.149 kb on - strand, within clpXat 4565.153 kb on - strandat 4565.175 kb on + strandat 4565.177 kb on + strandat 4565.178 kb on - strandat 4565.178 kb on - strandat 4565.380 kb on - strandat 4565.380 kb on - strandat 4565.411 kb on + strandat 4565.412 kb on - strandat 4565.412 kb on - strandat 4565.759 kb on - strand, within clpPat 4565.762 kb on + strand, within clpPat 4565.762 kb on + strand, within clpPat 4565.763 kb on - strand, within clpPat 4565.799 kb on - strand, within clpPat 4565.799 kb on - strand, within clpPat 4565.809 kb on + strand, within clpPat 4565.810 kb on - strand, within clpPat 4565.810 kb on - strand, within clpPat 4565.813 kb on + strand, within clpPat 4565.919 kb on + strand, within clpPat 4565.929 kb on + strand, within clpPat 4566.005 kb on + strandat 4566.022 kb on + strandat 4566.075 kb on - strandat 4566.075 kb on - strandat 4566.075 kb on - strandat 4566.080 kb on + strandat 4566.098 kb on + strandat 4566.140 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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4,563,136 + lon_P403L BNILDI_22670 0.71 -3.1
4,563,302 + lon_P403L BNILDI_22670 0.78 -1.4
4,563,883 + -3.0
4,563,894 - -1.2
4,563,894 - +0.2
4,563,894 - +0.5
4,563,902 - -0.9
4,563,903 + -2.0
4,563,985 + -0.3
4,563,985 + -0.3
4,564,044 - -0.6
4,564,076 - -0.8
4,564,128 - +1.9
4,564,128 - -0.7
4,564,154 - clpX BNILDI_22680 0.12 -2.3
4,564,770 - clpX BNILDI_22680 0.60 +1.2
4,564,770 - clpX BNILDI_22680 0.60 -0.9
4,565,148 + clpX BNILDI_22680 0.90 -3.0
4,565,149 - clpX BNILDI_22680 0.90 -0.7
4,565,153 - -1.7
4,565,175 + -0.9
4,565,177 + -2.1
4,565,178 - -2.8
4,565,178 - -2.2
4,565,380 - +0.5
4,565,380 - -0.1
4,565,411 + -1.2
4,565,412 - -1.5
4,565,412 - -0.7
4,565,759 - clpP BNILDI_22685 0.57 -1.7
4,565,762 + clpP BNILDI_22685 0.57 +0.9
4,565,762 + clpP BNILDI_22685 0.57 -0.1
4,565,763 - clpP BNILDI_22685 0.57 -3.0
4,565,799 - clpP BNILDI_22685 0.63 +0.0
4,565,799 - clpP BNILDI_22685 0.63 -2.0
4,565,809 + clpP BNILDI_22685 0.65 +0.7
4,565,810 - clpP BNILDI_22685 0.65 -1.2
4,565,810 - clpP BNILDI_22685 0.65 -1.5
4,565,813 + clpP BNILDI_22685 0.65 -2.6
4,565,919 + clpP BNILDI_22685 0.82 -2.7
4,565,929 + clpP BNILDI_22685 0.84 -2.5
4,566,005 + -2.9
4,566,022 + -2.8
4,566,075 - +0.4
4,566,075 - +0.3
4,566,075 - +0.1
4,566,080 + -1.1
4,566,098 + -0.9
4,566,140 + -0.0

Or see this region's nucleotide sequence