Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sdhA and sdhD overlap by 1 nucleotides sdhD and sdhC overlap by 7 nucleotides sdhC and ybgU are separated by 380 nucleotides ybgU and gltA are separated by 205 nucleotides
BNILDI_21320: sdhA - succinate dehydrogenase flavoprotein subunit, at 4,287,997 to 4,289,763
sdhA
BNILDI_21325: sdhD - succinate dehydrogenase membrane anchor subunit, at 4,289,763 to 4,290,110
sdhD
BNILDI_21330: sdhC - succinate dehydrogenase cytochrome b556 subunit, at 4,290,104 to 4,290,508
sdhC
BNILDI_21335: ybgU - Protein YbgU, at 4,290,889 to 4,290,996
ybgU
BNILDI_21340: gltA - citrate synthase, at 4,291,202 to 4,292,485
gltA
Position (kb) 4290
4291 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 4289.138 kb on + strand, within sdhA at 4289.139 kb on - strand, within sdhA at 4289.143 kb on - strand, within sdhA at 4289.143 kb on - strand, within sdhA at 4289.271 kb on - strand, within sdhA at 4289.420 kb on + strand, within sdhA at 4289.421 kb on - strand, within sdhA at 4289.425 kb on - strand, within sdhA at 4289.497 kb on - strand, within sdhA at 4289.502 kb on + strand, within sdhA at 4289.503 kb on - strand, within sdhA at 4289.503 kb on - strand, within sdhA at 4289.503 kb on - strand, within sdhA at 4289.504 kb on + strand, within sdhA at 4289.505 kb on - strand, within sdhA at 4289.556 kb on - strand, within sdhA at 4289.560 kb on - strand, within sdhA at 4289.574 kb on + strand, within sdhA at 4289.589 kb on + strand at 4289.652 kb on + strand at 4289.653 kb on - strand at 4289.792 kb on - strand at 4289.801 kb on - strand, within sdhD at 4289.977 kb on + strand, within sdhD at 4289.977 kb on + strand, within sdhD at 4289.978 kb on - strand, within sdhD at 4289.978 kb on - strand, within sdhD at 4289.978 kb on - strand, within sdhD at 4290.015 kb on - strand, within sdhD at 4290.016 kb on + strand, within sdhD at 4290.016 kb on + strand, within sdhD at 4290.016 kb on + strand, within sdhD at 4290.017 kb on - strand, within sdhD at 4290.017 kb on - strand, within sdhD at 4290.017 kb on - strand, within sdhD at 4290.017 kb on - strand, within sdhD at 4290.025 kb on + strand, within sdhD at 4290.025 kb on + strand, within sdhD at 4290.026 kb on - strand, within sdhD at 4290.111 kb on - strand at 4290.149 kb on - strand, within sdhC at 4290.149 kb on - strand, within sdhC at 4290.488 kb on + strand at 4290.517 kb on + strand at 4290.885 kb on + strand at 4290.887 kb on + strand at 4290.932 kb on + strand, within ybgU at 4290.933 kb on - strand, within ybgU at 4290.975 kb on + strand, within ybgU at 4291.010 kb on + strand at 4291.117 kb on - strand at 4291.288 kb on + strand at 4291.444 kb on + strand, within gltA at 4291.445 kb on - strand, within gltA at 4291.448 kb on + strand, within gltA at 4291.503 kb on - strand, within gltA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,289,138 + sdhA BNILDI_21320 0.65 -0.3 4,289,139 - sdhA BNILDI_21320 0.65 -3.8 4,289,143 - sdhA BNILDI_21320 0.65 -3.4 4,289,143 - sdhA BNILDI_21320 0.65 -3.9 4,289,271 - sdhA BNILDI_21320 0.72 -4.1 4,289,420 + sdhA BNILDI_21320 0.81 -3.0 4,289,421 - sdhA BNILDI_21320 0.81 -3.1 4,289,425 - sdhA BNILDI_21320 0.81 -3.2 4,289,497 - sdhA BNILDI_21320 0.85 -2.3 4,289,502 + sdhA BNILDI_21320 0.85 -1.6 4,289,503 - sdhA BNILDI_21320 0.85 -2.5 4,289,503 - sdhA BNILDI_21320 0.85 -4.9 4,289,503 - sdhA BNILDI_21320 0.85 -3.9 4,289,504 + sdhA BNILDI_21320 0.85 -1.3 4,289,505 - sdhA BNILDI_21320 0.85 -3.6 4,289,556 - sdhA BNILDI_21320 0.88 -3.8 4,289,560 - sdhA BNILDI_21320 0.88 -3.5 4,289,574 + sdhA BNILDI_21320 0.89 -1.4 4,289,589 + -3.8 4,289,652 + -4.3 4,289,653 - -2.8 4,289,792 - -3.4 4,289,801 - sdhD BNILDI_21325 0.11 -2.8 4,289,977 + sdhD BNILDI_21325 0.61 -2.2 4,289,977 + sdhD BNILDI_21325 0.61 -1.4 4,289,978 - sdhD BNILDI_21325 0.62 +3.5 4,289,978 - sdhD BNILDI_21325 0.62 +0.6 4,289,978 - sdhD BNILDI_21325 0.62 -0.9 4,290,015 - sdhD BNILDI_21325 0.72 -0.4 4,290,016 + sdhD BNILDI_21325 0.73 +0.3 4,290,016 + sdhD BNILDI_21325 0.73 -4.3 4,290,016 + sdhD BNILDI_21325 0.73 -3.8 4,290,017 - sdhD BNILDI_21325 0.73 -1.6 4,290,017 - sdhD BNILDI_21325 0.73 -2.2 4,290,017 - sdhD BNILDI_21325 0.73 -1.9 4,290,017 - sdhD BNILDI_21325 0.73 -3.4 4,290,025 + sdhD BNILDI_21325 0.75 -4.7 4,290,025 + sdhD BNILDI_21325 0.75 -4.2 4,290,026 - sdhD BNILDI_21325 0.76 -3.9 4,290,111 - -2.8 4,290,149 - sdhC BNILDI_21330 0.11 -1.1 4,290,149 - sdhC BNILDI_21330 0.11 -2.7 4,290,488 + +0.3 4,290,517 + -1.9 4,290,885 + +0.6 4,290,887 + +0.3 4,290,932 + ybgU BNILDI_21335 0.40 +0.1 4,290,933 - ybgU BNILDI_21335 0.41 +0.2 4,290,975 + ybgU BNILDI_21335 0.80 +0.6 4,291,010 + -1.6 4,291,117 - -0.4 4,291,288 + -1.1 4,291,444 + gltA BNILDI_21340 0.19 -1.8 4,291,445 - gltA BNILDI_21340 0.19 -1.2 4,291,448 + gltA BNILDI_21340 0.19 -2.2 4,291,503 - gltA BNILDI_21340 0.23 -1.3
Or see this region's nucleotide sequence