Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Lys_trna and cpoB are separated by 164 nucleotides cpoB and pal are separated by 9 nucleotides pal and tolB are separated by 34 nucleotides
BNILDI_21220: Lys_trna - tRNA-Lys(ttt), at 4,265,289 to 4,265,364
_trna
BNILDI_21225: cpoB - cell division protein CpoB, at 4,265,529 to 4,266,320
cpoB
BNILDI_21230: pal - peptidoglycan-associated lipoprotein Pal, at 4,266,330 to 4,266,851
pal
BNILDI_21235: tolB - Tol-Pal system protein TolB, at 4,266,886 to 4,268,178
tolB
Position (kb) 4266
4267 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 4265.500 kb on + strand at 4265.501 kb on - strand at 4265.530 kb on + strand at 4265.530 kb on + strand at 4265.530 kb on + strand at 4265.531 kb on - strand at 4265.531 kb on - strand at 4265.585 kb on + strand at 4265.585 kb on + strand at 4265.602 kb on + strand at 4265.603 kb on - strand at 4265.604 kb on + strand at 4265.717 kb on - strand, within cpoB at 4265.739 kb on - strand, within cpoB at 4265.750 kb on - strand, within cpoB at 4265.754 kb on + strand, within cpoB at 4265.755 kb on - strand, within cpoB at 4265.770 kb on + strand, within cpoB at 4265.873 kb on + strand, within cpoB at 4265.885 kb on - strand, within cpoB at 4266.179 kb on + strand, within cpoB at 4266.180 kb on - strand, within cpoB at 4266.267 kb on + strand at 4266.268 kb on - strand at 4266.269 kb on + strand at 4266.269 kb on + strand at 4266.295 kb on - strand at 4266.388 kb on - strand, within pal at 4266.457 kb on + strand, within pal at 4266.457 kb on + strand, within pal at 4266.457 kb on + strand, within pal at 4266.465 kb on - strand, within pal at 4266.538 kb on - strand, within pal at 4266.551 kb on - strand, within pal at 4266.551 kb on - strand, within pal at 4266.563 kb on + strand, within pal at 4266.620 kb on - strand, within pal at 4266.726 kb on - strand, within pal at 4267.013 kb on - strand at 4267.075 kb on + strand, within tolB at 4267.076 kb on - strand, within tolB at 4267.197 kb on + strand, within tolB at 4267.200 kb on + strand, within tolB at 4267.201 kb on - strand, within tolB at 4267.201 kb on - strand, within tolB at 4267.266 kb on + strand, within tolB at 4267.267 kb on - strand, within tolB at 4267.368 kb on - strand, within tolB at 4267.471 kb on + strand, within tolB at 4267.674 kb on - strand, within tolB at 4267.680 kb on + strand, within tolB at 4267.751 kb on + strand, within tolB at 4267.795 kb on + strand, within tolB at 4267.796 kb on - strand, within tolB at 4267.845 kb on - strand, within tolB
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,265,500 + +2.6 4,265,501 - -0.2 4,265,530 + +1.3 4,265,530 + -0.2 4,265,530 + +0.1 4,265,531 - -0.1 4,265,531 - +0.5 4,265,585 + -0.2 4,265,585 + +0.5 4,265,602 + -0.1 4,265,603 - -0.3 4,265,604 + +0.0 4,265,717 - cpoB BNILDI_21225 0.24 +0.2 4,265,739 - cpoB BNILDI_21225 0.27 -1.5 4,265,750 - cpoB BNILDI_21225 0.28 +1.9 4,265,754 + cpoB BNILDI_21225 0.28 +0.7 4,265,755 - cpoB BNILDI_21225 0.29 -1.6 4,265,770 + cpoB BNILDI_21225 0.30 -0.1 4,265,873 + cpoB BNILDI_21225 0.43 +1.0 4,265,885 - cpoB BNILDI_21225 0.45 -0.1 4,266,179 + cpoB BNILDI_21225 0.82 -0.2 4,266,180 - cpoB BNILDI_21225 0.82 -0.3 4,266,267 + +0.2 4,266,268 - -0.3 4,266,269 + +1.0 4,266,269 + +0.8 4,266,295 - -0.2 4,266,388 - pal BNILDI_21230 0.11 -0.7 4,266,457 + pal BNILDI_21230 0.24 -0.7 4,266,457 + pal BNILDI_21230 0.24 -2.1 4,266,457 + pal BNILDI_21230 0.24 +0.1 4,266,465 - pal BNILDI_21230 0.26 -1.4 4,266,538 - pal BNILDI_21230 0.40 -1.3 4,266,551 - pal BNILDI_21230 0.42 +0.3 4,266,551 - pal BNILDI_21230 0.42 -3.8 4,266,563 + pal BNILDI_21230 0.45 -0.5 4,266,620 - pal BNILDI_21230 0.56 -2.5 4,266,726 - pal BNILDI_21230 0.76 +0.5 4,267,013 - -0.9 4,267,075 + tolB BNILDI_21235 0.15 -2.0 4,267,076 - tolB BNILDI_21235 0.15 -1.5 4,267,197 + tolB BNILDI_21235 0.24 -4.0 4,267,200 + tolB BNILDI_21235 0.24 -2.4 4,267,201 - tolB BNILDI_21235 0.24 -1.3 4,267,201 - tolB BNILDI_21235 0.24 -3.3 4,267,266 + tolB BNILDI_21235 0.29 -1.4 4,267,267 - tolB BNILDI_21235 0.29 -1.1 4,267,368 - tolB BNILDI_21235 0.37 -1.9 4,267,471 + tolB BNILDI_21235 0.45 -3.6 4,267,674 - tolB BNILDI_21235 0.61 -1.9 4,267,680 + tolB BNILDI_21235 0.61 -2.6 4,267,751 + tolB BNILDI_21235 0.67 -2.3 4,267,795 + tolB BNILDI_21235 0.70 -2.3 4,267,796 - tolB BNILDI_21235 0.70 -0.4 4,267,845 - tolB BNILDI_21235 0.74 +0.9
Or see this region's nucleotide sequence