Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aroG and ybgS are separated by 315 nucleotides ybgS and zitB are separated by 113 nucleotides zitB and pnuC overlap by 4 nucleotides pnuC and nadA are separated by 37 nucleotides
BNILDI_21165: aroG - 3-deoxy-7-phosphoheptulonate synthase AroG, at 4,259,360 to 4,260,412
aroG
BNILDI_21170: ybgS - Uncharacterized protein YbgS, at 4,260,728 to 4,261,108
ybgS
BNILDI_21175: zitB - CDF family zinc transporter ZitB, at 4,261,222 to 4,262,163
zitB
BNILDI_21180: pnuC - nicotinamide riboside transporter PnuC, at 4,262,160 to 4,262,879
pnuC
BNILDI_21185: nadA - quinolinate synthase NadA, at 4,262,917 to 4,263,960
nadA
Position (kb)
4261
4262
4263 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4260.366 kb on - strand at 4260.465 kb on - strand at 4260.600 kb on + strand at 4260.661 kb on + strand at 4260.662 kb on - strand at 4260.662 kb on - strand at 4260.663 kb on + strand at 4260.663 kb on + strand at 4260.663 kb on + strand at 4260.663 kb on + strand at 4260.663 kb on + strand at 4260.663 kb on + strand at 4260.663 kb on + strand at 4260.664 kb on - strand at 4260.664 kb on - strand at 4260.704 kb on + strand at 4260.705 kb on - strand at 4260.772 kb on - strand, within ybgS at 4260.772 kb on - strand, within ybgS at 4260.820 kb on + strand, within ybgS at 4260.916 kb on - strand, within ybgS at 4261.034 kb on - strand, within ybgS at 4261.047 kb on + strand, within ybgS at 4261.177 kb on - strand at 4261.177 kb on - strand at 4261.192 kb on + strand at 4261.193 kb on - strand at 4261.266 kb on + strand at 4261.267 kb on - strand at 4261.267 kb on - strand at 4261.269 kb on + strand at 4261.288 kb on + strand at 4261.289 kb on - strand at 4261.289 kb on - strand at 4261.315 kb on - strand at 4261.381 kb on - strand, within zitB at 4261.381 kb on - strand, within zitB at 4261.520 kb on + strand, within zitB at 4261.521 kb on - strand, within zitB at 4261.626 kb on + strand, within zitB at 4261.628 kb on + strand, within zitB at 4261.644 kb on - strand, within zitB at 4261.680 kb on - strand, within zitB at 4261.680 kb on - strand, within zitB at 4261.749 kb on + strand, within zitB at 4261.749 kb on + strand, within zitB at 4261.857 kb on - strand, within zitB at 4261.905 kb on + strand, within zitB at 4261.946 kb on + strand, within zitB at 4261.946 kb on + strand, within zitB at 4261.946 kb on + strand, within zitB at 4261.946 kb on + strand, within zitB at 4261.947 kb on - strand, within zitB at 4261.947 kb on - strand, within zitB at 4261.947 kb on - strand, within zitB at 4261.947 kb on - strand, within zitB at 4261.947 kb on - strand, within zitB at 4261.947 kb on - strand, within zitB at 4261.952 kb on + strand, within zitB at 4261.952 kb on + strand, within zitB at 4261.952 kb on + strand, within zitB at 4261.952 kb on + strand, within zitB at 4261.952 kb on + strand, within zitB at 4261.952 kb on + strand, within zitB at 4261.953 kb on - strand, within zitB at 4262.249 kb on - strand, within pnuC at 4262.249 kb on - strand, within pnuC at 4262.310 kb on - strand, within pnuC at 4262.339 kb on - strand, within pnuC at 4262.342 kb on + strand, within pnuC at 4262.343 kb on - strand, within pnuC at 4262.370 kb on - strand, within pnuC at 4262.501 kb on + strand, within pnuC at 4262.624 kb on - strand, within pnuC at 4262.624 kb on - strand, within pnuC at 4262.664 kb on + strand, within pnuC at 4262.665 kb on - strand, within pnuC at 4262.738 kb on - strand, within pnuC at 4262.771 kb on + strand, within pnuC at 4262.771 kb on + strand, within pnuC at 4262.771 kb on + strand, within pnuC at 4262.772 kb on - strand, within pnuC at 4262.841 kb on + strand at 4262.841 kb on + strand at 4262.841 kb on + strand at 4262.841 kb on + strand at 4262.841 kb on + strand at 4262.841 kb on + strand at 4262.844 kb on - strand at 4262.844 kb on - strand at 4262.844 kb on - strand at 4262.844 kb on - strand at 4262.847 kb on + strand at 4262.847 kb on + strand at 4262.847 kb on + strand at 4262.848 kb on - strand at 4262.908 kb on + strand at 4262.909 kb on - strand at 4262.912 kb on - strand at 4262.912 kb on - strand at 4262.927 kb on - strand at 4262.927 kb on - strand at 4262.932 kb on - strand at 4262.950 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,260,366 - +0.2 4,260,465 - -0.6 4,260,600 + -1.0 4,260,661 + -0.1 4,260,662 - +0.6 4,260,662 - -0.1 4,260,663 + -0.5 4,260,663 + +0.8 4,260,663 + +0.1 4,260,663 + +0.1 4,260,663 + -0.0 4,260,663 + -0.3 4,260,663 + +0.0 4,260,664 - -0.2 4,260,664 - +0.1 4,260,704 + -0.5 4,260,705 - -1.5 4,260,772 - ybgS BNILDI_21170 0.12 +0.2 4,260,772 - ybgS BNILDI_21170 0.12 +1.2 4,260,820 + ybgS BNILDI_21170 0.24 -0.1 4,260,916 - ybgS BNILDI_21170 0.49 -1.1 4,261,034 - ybgS BNILDI_21170 0.80 +1.1 4,261,047 + ybgS BNILDI_21170 0.84 -1.2 4,261,177 - +0.5 4,261,177 - +1.9 4,261,192 + +0.4 4,261,193 - +0.2 4,261,266 + +0.5 4,261,267 - -0.5 4,261,267 - -0.7 4,261,269 + -0.9 4,261,288 + +2.1 4,261,289 - -0.9 4,261,289 - +0.7 4,261,315 - +0.3 4,261,381 - zitB BNILDI_21175 0.17 +0.5 4,261,381 - zitB BNILDI_21175 0.17 +1.2 4,261,520 + zitB BNILDI_21175 0.32 -1.0 4,261,521 - zitB BNILDI_21175 0.32 +1.4 4,261,626 + zitB BNILDI_21175 0.43 +0.2 4,261,628 + zitB BNILDI_21175 0.43 +0.9 4,261,644 - zitB BNILDI_21175 0.45 -0.3 4,261,680 - zitB BNILDI_21175 0.49 -1.1 4,261,680 - zitB BNILDI_21175 0.49 -0.1 4,261,749 + zitB BNILDI_21175 0.56 +0.1 4,261,749 + zitB BNILDI_21175 0.56 -1.0 4,261,857 - zitB BNILDI_21175 0.67 +0.2 4,261,905 + zitB BNILDI_21175 0.73 -0.4 4,261,946 + zitB BNILDI_21175 0.77 +0.1 4,261,946 + zitB BNILDI_21175 0.77 +0.3 4,261,946 + zitB BNILDI_21175 0.77 +0.6 4,261,946 + zitB BNILDI_21175 0.77 -0.4 4,261,947 - zitB BNILDI_21175 0.77 +0.0 4,261,947 - zitB BNILDI_21175 0.77 +0.4 4,261,947 - zitB BNILDI_21175 0.77 -2.7 4,261,947 - zitB BNILDI_21175 0.77 -2.3 4,261,947 - zitB BNILDI_21175 0.77 +0.2 4,261,947 - zitB BNILDI_21175 0.77 -0.0 4,261,952 + zitB BNILDI_21175 0.77 +0.3 4,261,952 + zitB BNILDI_21175 0.77 -1.1 4,261,952 + zitB BNILDI_21175 0.77 +1.2 4,261,952 + zitB BNILDI_21175 0.77 +1.0 4,261,952 + zitB BNILDI_21175 0.77 +1.2 4,261,952 + zitB BNILDI_21175 0.77 -0.3 4,261,953 - zitB BNILDI_21175 0.78 -0.7 4,262,249 - pnuC BNILDI_21180 0.12 -0.4 4,262,249 - pnuC BNILDI_21180 0.12 +0.1 4,262,310 - pnuC BNILDI_21180 0.21 -0.6 4,262,339 - pnuC BNILDI_21180 0.25 +0.7 4,262,342 + pnuC BNILDI_21180 0.25 -0.3 4,262,343 - pnuC BNILDI_21180 0.25 -1.0 4,262,370 - pnuC BNILDI_21180 0.29 -0.7 4,262,501 + pnuC BNILDI_21180 0.47 -1.2 4,262,624 - pnuC BNILDI_21180 0.64 -2.4 4,262,624 - pnuC BNILDI_21180 0.64 -0.2 4,262,664 + pnuC BNILDI_21180 0.70 +0.2 4,262,665 - pnuC BNILDI_21180 0.70 +0.4 4,262,738 - pnuC BNILDI_21180 0.80 -0.1 4,262,771 + pnuC BNILDI_21180 0.85 -1.2 4,262,771 + pnuC BNILDI_21180 0.85 -0.7 4,262,771 + pnuC BNILDI_21180 0.85 -0.1 4,262,772 - pnuC BNILDI_21180 0.85 -0.1 4,262,841 + -0.7 4,262,841 + -3.2 4,262,841 + -0.3 4,262,841 + -0.2 4,262,841 + +0.1 4,262,841 + +1.0 4,262,844 - -0.3 4,262,844 - -0.6 4,262,844 - -0.3 4,262,844 - -0.2 4,262,847 + -0.8 4,262,847 + +0.2 4,262,847 + +0.4 4,262,848 - -0.9 4,262,908 + +0.0 4,262,909 - -0.3 4,262,912 - +1.3 4,262,912 - +0.1 4,262,927 - -0.3 4,262,927 - +0.5 4,262,932 - -0.7 4,262,950 - -1.8
Or see this region's nucleotide sequence