Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rhlE and cecR are separated by 228 nucleotides cecR and hlyD are separated by 2 nucleotides hlyD and lptB overlap by 8 nucleotides
BNILDI_20925: rhlE - ATP-dependent RNA helicase RhlE, at 4,212,102 to 4,213,466
rhlE
BNILDI_20930: cecR - DNA-binding transcriptional regulator CecR, at 4,213,695 to 4,214,366
cecR
BNILDI_20935: hlyD - secretion protein HlyD, at 4,214,369 to 4,215,364
hlyD
BNILDI_20940: lptB - LPS export ABC transporter ATP-binding protein, at 4,215,357 to 4,217,093
lptB
Position (kb) 4214
4215
4216 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4213.406 kb on - strand at 4213.466 kb on + strand at 4213.466 kb on + strand at 4213.467 kb on - strand at 4213.470 kb on + strand at 4213.515 kb on + strand at 4213.515 kb on + strand at 4213.515 kb on + strand at 4213.516 kb on - strand at 4213.625 kb on - strand at 4213.700 kb on + strand at 4213.842 kb on + strand, within cecR at 4213.842 kb on + strand, within cecR at 4213.845 kb on + strand, within cecR at 4213.846 kb on - strand, within cecR at 4213.846 kb on - strand, within cecR at 4213.846 kb on - strand, within cecR at 4213.846 kb on - strand, within cecR at 4213.846 kb on - strand, within cecR at 4213.846 kb on - strand, within cecR at 4213.870 kb on - strand, within cecR at 4214.016 kb on - strand, within cecR at 4214.057 kb on + strand, within cecR at 4214.091 kb on + strand, within cecR at 4214.125 kb on + strand, within cecR at 4214.365 kb on - strand at 4214.406 kb on + strand at 4214.494 kb on + strand, within hlyD at 4214.494 kb on + strand, within hlyD at 4214.499 kb on + strand, within hlyD at 4214.603 kb on + strand, within hlyD at 4214.756 kb on + strand, within hlyD at 4214.756 kb on + strand, within hlyD at 4214.757 kb on - strand, within hlyD at 4214.758 kb on + strand, within hlyD at 4215.102 kb on - strand, within hlyD at 4215.155 kb on + strand, within hlyD at 4215.157 kb on + strand, within hlyD at 4215.269 kb on + strand at 4215.606 kb on + strand, within lptB at 4215.608 kb on + strand, within lptB at 4215.608 kb on + strand, within lptB at 4215.608 kb on + strand, within lptB at 4215.609 kb on - strand, within lptB at 4215.630 kb on + strand, within lptB at 4215.631 kb on - strand, within lptB at 4215.732 kb on - strand, within lptB at 4215.732 kb on - strand, within lptB at 4215.869 kb on + strand, within lptB at 4215.938 kb on + strand, within lptB at 4215.945 kb on + strand, within lptB at 4215.945 kb on + strand, within lptB at 4216.006 kb on - strand, within lptB at 4216.006 kb on - strand, within lptB at 4216.232 kb on + strand, within lptB at 4216.256 kb on + strand, within lptB at 4216.256 kb on + strand, within lptB at 4216.307 kb on - strand, within lptB at 4216.314 kb on - strand, within lptB at 4216.314 kb on - strand, within lptB at 4216.314 kb on - strand, within lptB at 4216.319 kb on - strand, within lptB
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,213,406 - +0.3 4,213,466 + +0.7 4,213,466 + -2.4 4,213,467 - +1.0 4,213,470 + +0.7 4,213,515 + -0.7 4,213,515 + +0.2 4,213,515 + -0.7 4,213,516 - -0.2 4,213,625 - +0.1 4,213,700 + -0.4 4,213,842 + cecR BNILDI_20930 0.22 -2.3 4,213,842 + cecR BNILDI_20930 0.22 +0.8 4,213,845 + cecR BNILDI_20930 0.22 +1.1 4,213,846 - cecR BNILDI_20930 0.22 -0.4 4,213,846 - cecR BNILDI_20930 0.22 -0.6 4,213,846 - cecR BNILDI_20930 0.22 -0.1 4,213,846 - cecR BNILDI_20930 0.22 -0.2 4,213,846 - cecR BNILDI_20930 0.22 +0.3 4,213,846 - cecR BNILDI_20930 0.22 +0.4 4,213,870 - cecR BNILDI_20930 0.26 +0.2 4,214,016 - cecR BNILDI_20930 0.48 +0.5 4,214,057 + cecR BNILDI_20930 0.54 +0.4 4,214,091 + cecR BNILDI_20930 0.59 +0.5 4,214,125 + cecR BNILDI_20930 0.64 +0.4 4,214,365 - -0.2 4,214,406 + -2.1 4,214,494 + hlyD BNILDI_20935 0.13 -1.2 4,214,494 + hlyD BNILDI_20935 0.13 -2.1 4,214,499 + hlyD BNILDI_20935 0.13 +0.4 4,214,603 + hlyD BNILDI_20935 0.23 +1.0 4,214,756 + hlyD BNILDI_20935 0.39 -1.9 4,214,756 + hlyD BNILDI_20935 0.39 +2.1 4,214,757 - hlyD BNILDI_20935 0.39 -0.1 4,214,758 + hlyD BNILDI_20935 0.39 +0.9 4,215,102 - hlyD BNILDI_20935 0.74 -0.1 4,215,155 + hlyD BNILDI_20935 0.79 -0.1 4,215,157 + hlyD BNILDI_20935 0.79 +0.7 4,215,269 + +0.6 4,215,606 + lptB BNILDI_20940 0.14 +0.5 4,215,608 + lptB BNILDI_20940 0.14 +0.2 4,215,608 + lptB BNILDI_20940 0.14 +0.7 4,215,608 + lptB BNILDI_20940 0.14 +0.5 4,215,609 - lptB BNILDI_20940 0.15 -0.2 4,215,630 + lptB BNILDI_20940 0.16 -0.7 4,215,631 - lptB BNILDI_20940 0.16 -0.3 4,215,732 - lptB BNILDI_20940 0.22 -0.3 4,215,732 - lptB BNILDI_20940 0.22 +0.2 4,215,869 + lptB BNILDI_20940 0.29 +0.3 4,215,938 + lptB BNILDI_20940 0.33 +0.2 4,215,945 + lptB BNILDI_20940 0.34 -0.1 4,215,945 + lptB BNILDI_20940 0.34 +0.8 4,216,006 - lptB BNILDI_20940 0.37 +0.1 4,216,006 - lptB BNILDI_20940 0.37 +0.5 4,216,232 + lptB BNILDI_20940 0.50 +1.7 4,216,256 + lptB BNILDI_20940 0.52 +1.3 4,216,256 + lptB BNILDI_20940 0.52 -0.5 4,216,307 - lptB BNILDI_20940 0.55 +0.6 4,216,314 - lptB BNILDI_20940 0.55 -0.4 4,216,314 - lptB BNILDI_20940 0.55 -2.4 4,216,314 - lptB BNILDI_20940 0.55 -0.2 4,216,319 - lptB BNILDI_20940 0.55 +0.6
Or see this region's nucleotide sequence