Strain Fitness in Escherichia coli ECRC62 around BNILDI_20260

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntkdsB and ycaR overlap by 4 nucleotidesycaR and ycaQ are separated by 51 nucleotidesycaQ and lpxK are separated by 36 nucleotides BNILDI_20250: kdsB - 3-deoxy-manno-octulosonate cytidylyltransferase, at 4,070,076 to 4,070,822 kdsB BNILDI_20255: ycaR - protein YcaR, at 4,070,819 to 4,071,001 ycaR BNILDI_20260: ycaQ - Uncharacterized protein YcaQ, at 4,071,053 to 4,072,285 ycaQ BNILDI_20265: lpxK - tetraacyldisaccharide 4'-kinase, at 4,072,322 to 4,073,308 lpxK Position (kb) 4071 4072 4073Strain fitness (log2 ratio) -1 0 1 2at 4070.078 kb on - strandat 4071.035 kb on - strandat 4071.047 kb on - strandat 4071.055 kb on - strandat 4071.203 kb on - strand, within ycaQat 4071.230 kb on - strand, within ycaQat 4071.230 kb on - strand, within ycaQat 4071.281 kb on + strand, within ycaQat 4071.281 kb on + strand, within ycaQat 4071.282 kb on - strand, within ycaQat 4071.296 kb on + strand, within ycaQat 4071.296 kb on + strand, within ycaQat 4071.296 kb on + strand, within ycaQat 4071.297 kb on - strand, within ycaQat 4071.297 kb on - strand, within ycaQat 4071.300 kb on - strand, within ycaQat 4071.315 kb on + strand, within ycaQat 4071.316 kb on - strand, within ycaQat 4071.316 kb on - strand, within ycaQat 4071.321 kb on + strand, within ycaQat 4071.344 kb on - strand, within ycaQat 4071.345 kb on + strand, within ycaQat 4071.346 kb on - strand, within ycaQat 4071.623 kb on + strand, within ycaQat 4071.725 kb on + strand, within ycaQat 4071.726 kb on - strand, within ycaQat 4071.732 kb on + strand, within ycaQat 4071.786 kb on - strand, within ycaQat 4071.786 kb on - strand, within ycaQat 4071.786 kb on - strand, within ycaQat 4071.880 kb on + strand, within ycaQat 4071.889 kb on + strand, within ycaQat 4071.890 kb on - strand, within ycaQat 4071.890 kb on - strand, within ycaQat 4071.890 kb on - strand, within ycaQat 4071.890 kb on - strand, within ycaQat 4071.945 kb on + strand, within ycaQat 4072.012 kb on + strand, within ycaQat 4072.117 kb on - strand, within ycaQat 4072.124 kb on - strand, within ycaQat 4072.136 kb on - strand, within ycaQat 4072.136 kb on - strand, within ycaQat 4072.323 kb on + strandat 4072.323 kb on + strandat 4072.323 kb on + strandat 4072.323 kb on + strandat 4072.323 kb on + strandat 4072.324 kb on - strandat 4072.324 kb on - strandat 4072.347 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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4,070,078 - +0.2
4,071,035 - +0.3
4,071,047 - +0.0
4,071,055 - -0.5
4,071,203 - ycaQ BNILDI_20260 0.12 -0.0
4,071,230 - ycaQ BNILDI_20260 0.14 +0.3
4,071,230 - ycaQ BNILDI_20260 0.14 -1.3
4,071,281 + ycaQ BNILDI_20260 0.18 -0.1
4,071,281 + ycaQ BNILDI_20260 0.18 +0.8
4,071,282 - ycaQ BNILDI_20260 0.19 +0.2
4,071,296 + ycaQ BNILDI_20260 0.20 -0.4
4,071,296 + ycaQ BNILDI_20260 0.20 -0.3
4,071,296 + ycaQ BNILDI_20260 0.20 +0.3
4,071,297 - ycaQ BNILDI_20260 0.20 +0.6
4,071,297 - ycaQ BNILDI_20260 0.20 -1.0
4,071,300 - ycaQ BNILDI_20260 0.20 -0.3
4,071,315 + ycaQ BNILDI_20260 0.21 -0.3
4,071,316 - ycaQ BNILDI_20260 0.21 +0.0
4,071,316 - ycaQ BNILDI_20260 0.21 -0.0
4,071,321 + ycaQ BNILDI_20260 0.22 +1.3
4,071,344 - ycaQ BNILDI_20260 0.24 -0.2
4,071,345 + ycaQ BNILDI_20260 0.24 +0.8
4,071,346 - ycaQ BNILDI_20260 0.24 -0.3
4,071,623 + ycaQ BNILDI_20260 0.46 +1.0
4,071,725 + ycaQ BNILDI_20260 0.55 +0.8
4,071,726 - ycaQ BNILDI_20260 0.55 +0.0
4,071,732 + ycaQ BNILDI_20260 0.55 +1.0
4,071,786 - ycaQ BNILDI_20260 0.59 +0.3
4,071,786 - ycaQ BNILDI_20260 0.59 -0.0
4,071,786 - ycaQ BNILDI_20260 0.59 +2.1
4,071,880 + ycaQ BNILDI_20260 0.67 -0.6
4,071,889 + ycaQ BNILDI_20260 0.68 +1.8
4,071,890 - ycaQ BNILDI_20260 0.68 +0.9
4,071,890 - ycaQ BNILDI_20260 0.68 +1.2
4,071,890 - ycaQ BNILDI_20260 0.68 -0.1
4,071,890 - ycaQ BNILDI_20260 0.68 -0.3
4,071,945 + ycaQ BNILDI_20260 0.72 -0.7
4,072,012 + ycaQ BNILDI_20260 0.78 +0.1
4,072,117 - ycaQ BNILDI_20260 0.86 +0.0
4,072,124 - ycaQ BNILDI_20260 0.87 -0.8
4,072,136 - ycaQ BNILDI_20260 0.88 -1.0
4,072,136 - ycaQ BNILDI_20260 0.88 -0.2
4,072,323 + -0.1
4,072,323 + -0.6
4,072,323 + +0.8
4,072,323 + -1.0
4,072,323 + +0.5
4,072,324 - -0.2
4,072,324 - -0.1
4,072,347 - -0.3

Or see this region's nucleotide sequence