Strain Fitness in Escherichia coli ECRC62 around BNILDI_18150

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycjG and tpx are separated by 118 nucleotidestpx and tyrR are separated by 43 nucleotidestyrR and ycjF are separated by 147 nucleotides BNILDI_18140: ycjG - L-Ala-D/L-Glu epimerase, at 3,652,937 to 3,653,902 ycjG BNILDI_18145: tpx - thiol peroxidase, at 3,654,021 to 3,654,527 tpx BNILDI_18150: tyrR - transcriptional regulator TyrR, at 3,654,571 to 3,656,112 tyrR BNILDI_18155: ycjF - UPF0283 membrane protein YcjF, at 3,656,260 to 3,657,321 ycjF Position (kb) 3654 3655 3656 3657Strain fitness (log2 ratio) -2 -1 0 1 2at 3653.638 kb on + strand, within ycjGat 3653.638 kb on + strand, within ycjGat 3653.683 kb on + strand, within ycjGat 3653.860 kb on + strandat 3653.861 kb on - strandat 3653.861 kb on - strandat 3653.864 kb on + strandat 3653.886 kb on - strandat 3653.886 kb on - strandat 3653.977 kb on - strandat 3653.999 kb on + strandat 3654.016 kb on + strandat 3654.214 kb on + strand, within tpxat 3654.218 kb on + strand, within tpxat 3654.218 kb on + strand, within tpxat 3654.218 kb on + strand, within tpxat 3654.219 kb on - strand, within tpxat 3654.372 kb on + strand, within tpxat 3654.431 kb on + strand, within tpxat 3654.495 kb on + strandat 3654.496 kb on - strandat 3654.525 kb on + strandat 3654.526 kb on - strandat 3654.530 kb on + strandat 3654.531 kb on - strandat 3654.531 kb on - strandat 3654.534 kb on - strandat 3654.573 kb on - strandat 3654.573 kb on - strandat 3654.603 kb on - strandat 3654.631 kb on - strandat 3654.674 kb on + strandat 3654.674 kb on + strandat 3654.895 kb on + strand, within tyrRat 3654.895 kb on + strand, within tyrRat 3654.896 kb on - strand, within tyrRat 3654.900 kb on + strand, within tyrRat 3654.901 kb on - strand, within tyrRat 3655.044 kb on - strand, within tyrRat 3655.044 kb on - strand, within tyrRat 3655.044 kb on - strand, within tyrRat 3655.044 kb on - strand, within tyrRat 3655.317 kb on + strand, within tyrRat 3655.318 kb on - strand, within tyrRat 3655.804 kb on - strand, within tyrRat 3656.053 kb on + strandat 3656.053 kb on + strandat 3656.142 kb on - strandat 3656.142 kb on - strandat 3656.153 kb on - strandat 3656.161 kb on - strandat 3656.235 kb on + strandat 3656.386 kb on - strand, within ycjFat 3656.608 kb on + strand, within ycjFat 3656.726 kb on - strand, within ycjFat 3656.798 kb on + strand, within ycjF

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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3,653,638 + ycjG BNILDI_18140 0.73 -2.4
3,653,638 + ycjG BNILDI_18140 0.73 +0.4
3,653,683 + ycjG BNILDI_18140 0.77 -0.7
3,653,860 + +0.5
3,653,861 - -1.1
3,653,861 - +1.9
3,653,864 + -0.3
3,653,886 - +0.4
3,653,886 - -0.3
3,653,977 - +0.8
3,653,999 + -0.1
3,654,016 + +1.0
3,654,214 + tpx BNILDI_18145 0.38 +0.5
3,654,218 + tpx BNILDI_18145 0.39 -0.1
3,654,218 + tpx BNILDI_18145 0.39 -0.8
3,654,218 + tpx BNILDI_18145 0.39 +0.1
3,654,219 - tpx BNILDI_18145 0.39 -0.2
3,654,372 + tpx BNILDI_18145 0.69 +0.1
3,654,431 + tpx BNILDI_18145 0.81 -0.9
3,654,495 + -2.0
3,654,496 - -0.2
3,654,525 + -0.2
3,654,526 - +1.6
3,654,530 + +0.0
3,654,531 - -0.2
3,654,531 - -0.3
3,654,534 - -0.8
3,654,573 - +0.3
3,654,573 - -0.2
3,654,603 - +0.1
3,654,631 - -0.2
3,654,674 + -0.5
3,654,674 + -0.2
3,654,895 + tyrR BNILDI_18150 0.21 -1.9
3,654,895 + tyrR BNILDI_18150 0.21 +1.7
3,654,896 - tyrR BNILDI_18150 0.21 -0.2
3,654,900 + tyrR BNILDI_18150 0.21 +0.6
3,654,901 - tyrR BNILDI_18150 0.21 -0.8
3,655,044 - tyrR BNILDI_18150 0.31 +0.2
3,655,044 - tyrR BNILDI_18150 0.31 -0.2
3,655,044 - tyrR BNILDI_18150 0.31 +0.5
3,655,044 - tyrR BNILDI_18150 0.31 +0.9
3,655,317 + tyrR BNILDI_18150 0.48 -1.3
3,655,318 - tyrR BNILDI_18150 0.48 -0.4
3,655,804 - tyrR BNILDI_18150 0.80 -0.1
3,656,053 + +0.1
3,656,053 + -0.5
3,656,142 - +1.0
3,656,142 - -0.6
3,656,153 - +1.9
3,656,161 - +0.2
3,656,235 + -0.2
3,656,386 - ycjF BNILDI_18155 0.12 -0.4
3,656,608 + ycjF BNILDI_18155 0.33 +0.1
3,656,726 - ycjF BNILDI_18155 0.44 -0.3
3,656,798 + ycjF BNILDI_18155 0.51 -0.2

Or see this region's nucleotide sequence