Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycjG and tpx are separated by 118 nucleotides tpx and tyrR are separated by 43 nucleotides tyrR and ycjF are separated by 147 nucleotides
BNILDI_18140: ycjG - L-Ala-D/L-Glu epimerase, at 3,652,937 to 3,653,902
ycjG
BNILDI_18145: tpx - thiol peroxidase, at 3,654,021 to 3,654,527
tpx
BNILDI_18150: tyrR - transcriptional regulator TyrR, at 3,654,571 to 3,656,112
tyrR
BNILDI_18155: ycjF - UPF0283 membrane protein YcjF, at 3,656,260 to 3,657,321
ycjF
Position (kb)
3654
3655
3656
3657 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3653.638 kb on + strand, within ycjG at 3653.638 kb on + strand, within ycjG at 3653.683 kb on + strand, within ycjG at 3653.860 kb on + strand at 3653.861 kb on - strand at 3653.861 kb on - strand at 3653.864 kb on + strand at 3653.886 kb on - strand at 3653.886 kb on - strand at 3653.977 kb on - strand at 3653.999 kb on + strand at 3654.016 kb on + strand at 3654.214 kb on + strand, within tpx at 3654.218 kb on + strand, within tpx at 3654.218 kb on + strand, within tpx at 3654.218 kb on + strand, within tpx at 3654.219 kb on - strand, within tpx at 3654.372 kb on + strand, within tpx at 3654.431 kb on + strand, within tpx at 3654.495 kb on + strand at 3654.496 kb on - strand at 3654.525 kb on + strand at 3654.526 kb on - strand at 3654.530 kb on + strand at 3654.531 kb on - strand at 3654.531 kb on - strand at 3654.534 kb on - strand at 3654.573 kb on - strand at 3654.573 kb on - strand at 3654.603 kb on - strand at 3654.631 kb on - strand at 3654.674 kb on + strand at 3654.674 kb on + strand at 3654.895 kb on + strand, within tyrR at 3654.895 kb on + strand, within tyrR at 3654.896 kb on - strand, within tyrR at 3654.900 kb on + strand, within tyrR at 3654.901 kb on - strand, within tyrR at 3655.044 kb on - strand, within tyrR at 3655.044 kb on - strand, within tyrR at 3655.044 kb on - strand, within tyrR at 3655.044 kb on - strand, within tyrR at 3655.317 kb on + strand, within tyrR at 3655.318 kb on - strand, within tyrR at 3655.804 kb on - strand, within tyrR at 3656.053 kb on + strand at 3656.053 kb on + strand at 3656.142 kb on - strand at 3656.142 kb on - strand at 3656.153 kb on - strand at 3656.161 kb on - strand at 3656.235 kb on + strand at 3656.386 kb on - strand, within ycjF at 3656.608 kb on + strand, within ycjF at 3656.726 kb on - strand, within ycjF at 3656.798 kb on + strand, within ycjF
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,653,638 + ycjG BNILDI_18140 0.73 -2.4 3,653,638 + ycjG BNILDI_18140 0.73 +0.4 3,653,683 + ycjG BNILDI_18140 0.77 -0.7 3,653,860 + +0.5 3,653,861 - -1.1 3,653,861 - +1.9 3,653,864 + -0.3 3,653,886 - +0.4 3,653,886 - -0.3 3,653,977 - +0.8 3,653,999 + -0.1 3,654,016 + +1.0 3,654,214 + tpx BNILDI_18145 0.38 +0.5 3,654,218 + tpx BNILDI_18145 0.39 -0.1 3,654,218 + tpx BNILDI_18145 0.39 -0.8 3,654,218 + tpx BNILDI_18145 0.39 +0.1 3,654,219 - tpx BNILDI_18145 0.39 -0.2 3,654,372 + tpx BNILDI_18145 0.69 +0.1 3,654,431 + tpx BNILDI_18145 0.81 -0.9 3,654,495 + -2.0 3,654,496 - -0.2 3,654,525 + -0.2 3,654,526 - +1.6 3,654,530 + +0.0 3,654,531 - -0.2 3,654,531 - -0.3 3,654,534 - -0.8 3,654,573 - +0.3 3,654,573 - -0.2 3,654,603 - +0.1 3,654,631 - -0.2 3,654,674 + -0.5 3,654,674 + -0.2 3,654,895 + tyrR BNILDI_18150 0.21 -1.9 3,654,895 + tyrR BNILDI_18150 0.21 +1.7 3,654,896 - tyrR BNILDI_18150 0.21 -0.2 3,654,900 + tyrR BNILDI_18150 0.21 +0.6 3,654,901 - tyrR BNILDI_18150 0.21 -0.8 3,655,044 - tyrR BNILDI_18150 0.31 +0.2 3,655,044 - tyrR BNILDI_18150 0.31 -0.2 3,655,044 - tyrR BNILDI_18150 0.31 +0.5 3,655,044 - tyrR BNILDI_18150 0.31 +0.9 3,655,317 + tyrR BNILDI_18150 0.48 -1.3 3,655,318 - tyrR BNILDI_18150 0.48 -0.4 3,655,804 - tyrR BNILDI_18150 0.80 -0.1 3,656,053 + +0.1 3,656,053 + -0.5 3,656,142 - +1.0 3,656,142 - -0.6 3,656,153 - +1.9 3,656,161 - +0.2 3,656,235 + -0.2 3,656,386 - ycjF BNILDI_18155 0.12 -0.4 3,656,608 + ycjF BNILDI_18155 0.33 +0.1 3,656,726 - ycjF BNILDI_18155 0.44 -0.3 3,656,798 + ycjF BNILDI_18155 0.51 -0.2
Or see this region's nucleotide sequence