Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt uspE and ynaJ are separated by 49 nucleotides ynaJ and ynaI are separated by 243 nucleotides
BNILDI_18095: uspE - universal stress protein UspE, at 3,643,864 to 3,644,814
uspE
BNILDI_18100: ynaJ - Uncharacterized protein YnaJ, at 3,644,864 to 3,645,121
ynaJ
BNILDI_18105: ynaI - low conductance mechanosensitive channel YnaI, at 3,645,365 to 3,646,396
ynaI
Position (kb)
3644
3645
3646 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3643.874 kb on - strand at 3643.940 kb on - strand at 3643.943 kb on + strand at 3643.943 kb on + strand at 3643.987 kb on + strand, within uspE at 3643.988 kb on - strand, within uspE at 3644.040 kb on + strand, within uspE at 3644.095 kb on + strand, within uspE at 3644.096 kb on - strand, within uspE at 3644.370 kb on + strand, within uspE at 3644.472 kb on + strand, within uspE at 3644.515 kb on - strand, within uspE at 3644.646 kb on - strand, within uspE at 3644.689 kb on - strand, within uspE at 3644.760 kb on + strand at 3644.760 kb on + strand at 3644.862 kb on - strand at 3644.866 kb on - strand at 3644.866 kb on - strand at 3644.890 kb on - strand, within ynaJ at 3644.920 kb on + strand, within ynaJ at 3645.000 kb on + strand, within ynaJ at 3645.242 kb on - strand at 3645.258 kb on + strand at 3645.389 kb on - strand at 3645.548 kb on - strand, within ynaI at 3645.551 kb on + strand, within ynaI at 3645.558 kb on + strand, within ynaI at 3645.946 kb on + strand, within ynaI at 3645.946 kb on + strand, within ynaI at 3646.007 kb on + strand, within ynaI
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,643,874 - -1.9 3,643,940 - +0.6 3,643,943 + +0.2 3,643,943 + -2.4 3,643,987 + uspE BNILDI_18095 0.13 -0.2 3,643,988 - uspE BNILDI_18095 0.13 +0.8 3,644,040 + uspE BNILDI_18095 0.19 +1.0 3,644,095 + uspE BNILDI_18095 0.24 +0.3 3,644,096 - uspE BNILDI_18095 0.24 -3.1 3,644,370 + uspE BNILDI_18095 0.53 +0.0 3,644,472 + uspE BNILDI_18095 0.64 +0.1 3,644,515 - uspE BNILDI_18095 0.68 +0.4 3,644,646 - uspE BNILDI_18095 0.82 +0.9 3,644,689 - uspE BNILDI_18095 0.87 +1.0 3,644,760 + -0.3 3,644,760 + -1.6 3,644,862 - +0.2 3,644,866 - +0.9 3,644,866 - -0.4 3,644,890 - ynaJ BNILDI_18100 0.10 -1.3 3,644,920 + ynaJ BNILDI_18100 0.22 +0.4 3,645,000 + ynaJ BNILDI_18100 0.53 -0.2 3,645,242 - +1.3 3,645,258 + +0.6 3,645,389 - +0.4 3,645,548 - ynaI BNILDI_18105 0.18 +0.6 3,645,551 + ynaI BNILDI_18105 0.18 -0.4 3,645,558 + ynaI BNILDI_18105 0.19 -1.2 3,645,946 + ynaI BNILDI_18105 0.56 +0.1 3,645,946 + ynaI BNILDI_18105 0.56 -0.8 3,646,007 + ynaI BNILDI_18105 0.62 -0.1
Or see this region's nucleotide sequence