Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aldA and ydcF are separated by 196 nucleotides ydcF and hrpA are separated by 271 nucleotides
BNILDI_17835: aldA - aldehyde dehydrogenase, at 3,584,808 to 3,586,247
aldA
BNILDI_17840: ydcF - Protein YdcF, at 3,586,444 to 3,587,244
ydcF
BNILDI_17845: hrpA - ATP-dependent RNA helicase HrpA, at 3,587,516 to 3,591,418
hrpA
Position (kb)
3586
3587
3588 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3585.469 kb on - strand, within aldA at 3585.633 kb on + strand, within aldA at 3585.664 kb on - strand, within aldA at 3585.734 kb on + strand, within aldA at 3585.734 kb on + strand, within aldA at 3585.915 kb on - strand, within aldA at 3585.916 kb on + strand, within aldA at 3585.916 kb on + strand, within aldA at 3585.916 kb on + strand, within aldA at 3586.199 kb on + strand at 3586.199 kb on + strand at 3586.219 kb on + strand at 3586.219 kb on + strand at 3586.220 kb on - strand at 3586.220 kb on - strand at 3586.220 kb on - strand at 3586.220 kb on - strand at 3586.223 kb on + strand at 3586.223 kb on + strand at 3586.223 kb on + strand at 3586.223 kb on + strand at 3586.224 kb on - strand at 3586.224 kb on - strand at 3586.224 kb on - strand at 3586.224 kb on - strand at 3586.368 kb on - strand at 3586.368 kb on - strand at 3586.578 kb on + strand, within ydcF at 3586.640 kb on - strand, within ydcF at 3586.667 kb on - strand, within ydcF at 3586.714 kb on + strand, within ydcF at 3586.838 kb on - strand, within ydcF at 3587.079 kb on + strand, within ydcF at 3587.080 kb on - strand, within ydcF at 3587.194 kb on + strand at 3587.195 kb on - strand at 3587.203 kb on + strand at 3587.203 kb on + strand at 3587.204 kb on - strand at 3587.204 kb on - strand at 3587.205 kb on + strand at 3587.205 kb on + strand at 3587.205 kb on + strand at 3587.284 kb on + strand at 3587.287 kb on + strand at 3587.323 kb on + strand at 3587.324 kb on - strand at 3587.324 kb on - strand at 3587.324 kb on - strand at 3587.357 kb on - strand at 3587.376 kb on + strand at 3587.381 kb on - strand at 3587.387 kb on + strand at 3587.449 kb on + strand at 3587.455 kb on + strand at 3587.548 kb on + strand at 3587.596 kb on - strand at 3587.616 kb on - strand at 3587.841 kb on - strand at 3588.064 kb on + strand, within hrpA at 3588.083 kb on - strand, within hrpA at 3588.126 kb on + strand, within hrpA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,585,469 - aldA BNILDI_17835 0.46 +0.1 3,585,633 + aldA BNILDI_17835 0.57 -2.1 3,585,664 - aldA BNILDI_17835 0.59 +0.4 3,585,734 + aldA BNILDI_17835 0.64 +0.2 3,585,734 + aldA BNILDI_17835 0.64 +1.3 3,585,915 - aldA BNILDI_17835 0.77 +0.3 3,585,916 + aldA BNILDI_17835 0.77 -0.2 3,585,916 + aldA BNILDI_17835 0.77 +0.9 3,585,916 + aldA BNILDI_17835 0.77 +0.0 3,586,199 + +0.3 3,586,199 + +0.4 3,586,219 + +0.3 3,586,219 + -0.4 3,586,220 - -0.1 3,586,220 - -1.9 3,586,220 - -0.1 3,586,220 - +0.8 3,586,223 + -0.0 3,586,223 + +0.6 3,586,223 + -3.1 3,586,223 + -0.7 3,586,224 - -0.6 3,586,224 - -0.2 3,586,224 - +0.6 3,586,224 - +0.2 3,586,368 - -1.9 3,586,368 - +0.8 3,586,578 + ydcF BNILDI_17840 0.17 -0.0 3,586,640 - ydcF BNILDI_17840 0.24 +0.5 3,586,667 - ydcF BNILDI_17840 0.28 -0.6 3,586,714 + ydcF BNILDI_17840 0.34 -0.8 3,586,838 - ydcF BNILDI_17840 0.49 -0.8 3,587,079 + ydcF BNILDI_17840 0.79 -1.6 3,587,080 - ydcF BNILDI_17840 0.79 -1.3 3,587,194 + -1.5 3,587,195 - +0.2 3,587,203 + -0.8 3,587,203 + +0.0 3,587,204 - -1.0 3,587,204 - -0.3 3,587,205 + +0.0 3,587,205 + -0.5 3,587,205 + -0.3 3,587,284 + -0.4 3,587,287 + -2.2 3,587,323 + +0.2 3,587,324 - +0.3 3,587,324 - +0.0 3,587,324 - -0.7 3,587,357 - -0.9 3,587,376 + -0.7 3,587,381 - +0.3 3,587,387 + -2.3 3,587,449 + -0.7 3,587,455 + -1.2 3,587,548 + -0.2 3,587,596 - +0.1 3,587,616 - -0.5 3,587,841 - -0.0 3,588,064 + hrpA BNILDI_17845 0.14 +0.2 3,588,083 - hrpA BNILDI_17845 0.15 +1.3 3,588,126 + hrpA BNILDI_17845 0.16 +0.2
Or see this region's nucleotide sequence