Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glsB and yneG overlap by 1 nucleotides yneG and gGDEF are separated by 138 nucleotides gGDEF and uxaB are separated by 226 nucleotides
BNILDI_17245: glsB - glutaminase B, at 3,453,861 to 3,454,787
glsB
BNILDI_17250: yneG - Uncharacterized protein YneG, at 3,454,787 to 3,455,146
yneG
BNILDI_17255: gGDEF - diguanylate cyclase, at 3,455,285 to 3,456,703
gGDEF
BNILDI_17260: uxaB - tagaturonate reductase, at 3,456,930 to 3,458,381
uxaB
Position (kb)
3455
3456
3457 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3454.310 kb on + strand, within glsB at 3454.310 kb on + strand, within glsB at 3454.317 kb on + strand, within glsB at 3454.521 kb on - strand, within glsB at 3454.586 kb on + strand, within glsB at 3454.586 kb on + strand, within glsB at 3454.587 kb on - strand, within glsB at 3454.722 kb on - strand at 3454.782 kb on + strand at 3454.783 kb on - strand at 3454.785 kb on + strand at 3454.872 kb on - strand, within yneG at 3455.051 kb on - strand, within yneG at 3455.093 kb on + strand, within yneG at 3455.093 kb on + strand, within yneG at 3455.094 kb on - strand, within yneG at 3455.094 kb on - strand, within yneG at 3455.094 kb on - strand, within yneG at 3455.095 kb on + strand, within yneG at 3455.096 kb on - strand, within yneG at 3455.096 kb on - strand, within yneG at 3455.096 kb on - strand, within yneG at 3455.128 kb on - strand at 3455.128 kb on - strand at 3455.168 kb on + strand at 3455.168 kb on + strand at 3455.224 kb on - strand at 3455.270 kb on - strand at 3455.292 kb on + strand at 3455.293 kb on - strand at 3455.341 kb on + strand at 3455.342 kb on - strand at 3455.344 kb on + strand at 3455.344 kb on + strand at 3455.345 kb on - strand at 3455.378 kb on + strand at 3455.379 kb on - strand at 3455.466 kb on - strand, within gGDEF at 3455.466 kb on - strand, within gGDEF at 3455.476 kb on + strand, within gGDEF at 3455.489 kb on + strand, within gGDEF at 3455.489 kb on + strand, within gGDEF at 3455.490 kb on - strand, within gGDEF at 3455.490 kb on - strand, within gGDEF at 3455.523 kb on - strand, within gGDEF at 3455.643 kb on - strand, within gGDEF at 3455.671 kb on + strand, within gGDEF at 3455.672 kb on - strand, within gGDEF at 3455.697 kb on + strand, within gGDEF at 3455.705 kb on - strand, within gGDEF at 3455.705 kb on - strand, within gGDEF at 3455.812 kb on + strand, within gGDEF at 3455.812 kb on + strand, within gGDEF at 3455.813 kb on - strand, within gGDEF at 3455.974 kb on + strand, within gGDEF at 3456.053 kb on - strand, within gGDEF at 3456.218 kb on + strand, within gGDEF at 3456.236 kb on + strand, within gGDEF at 3456.306 kb on - strand, within gGDEF at 3456.330 kb on - strand, within gGDEF at 3456.330 kb on - strand, within gGDEF at 3456.539 kb on + strand, within gGDEF at 3456.554 kb on - strand, within gGDEF at 3456.783 kb on + strand at 3456.784 kb on - strand at 3457.001 kb on + strand at 3457.134 kb on + strand, within uxaB at 3457.135 kb on - strand, within uxaB at 3457.137 kb on - strand, within uxaB at 3457.137 kb on - strand, within uxaB at 3457.224 kb on + strand, within uxaB at 3457.289 kb on + strand, within uxaB at 3457.289 kb on + strand, within uxaB at 3457.311 kb on + strand, within uxaB
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,454,310 + glsB BNILDI_17245 0.48 +0.5 3,454,310 + glsB BNILDI_17245 0.48 +0.6 3,454,317 + glsB BNILDI_17245 0.49 +0.7 3,454,521 - glsB BNILDI_17245 0.71 +0.0 3,454,586 + glsB BNILDI_17245 0.78 +0.0 3,454,586 + glsB BNILDI_17245 0.78 -1.5 3,454,587 - glsB BNILDI_17245 0.78 -2.9 3,454,722 - +0.5 3,454,782 + -0.2 3,454,783 - -1.9 3,454,785 + -0.8 3,454,872 - yneG BNILDI_17250 0.24 -0.1 3,455,051 - yneG BNILDI_17250 0.73 -2.9 3,455,093 + yneG BNILDI_17250 0.85 +0.0 3,455,093 + yneG BNILDI_17250 0.85 -0.1 3,455,094 - yneG BNILDI_17250 0.85 +1.0 3,455,094 - yneG BNILDI_17250 0.85 +0.6 3,455,094 - yneG BNILDI_17250 0.85 +0.1 3,455,095 + yneG BNILDI_17250 0.86 +0.4 3,455,096 - yneG BNILDI_17250 0.86 -1.6 3,455,096 - yneG BNILDI_17250 0.86 -2.9 3,455,096 - yneG BNILDI_17250 0.86 -0.2 3,455,128 - +0.4 3,455,128 - +0.5 3,455,168 + -0.2 3,455,168 + -0.6 3,455,224 - +0.3 3,455,270 - -1.2 3,455,292 + -0.2 3,455,293 - -1.5 3,455,341 + +0.8 3,455,342 - -0.3 3,455,344 + -0.0 3,455,344 + +0.1 3,455,345 - -0.6 3,455,378 + -0.3 3,455,379 - +0.2 3,455,466 - gGDEF BNILDI_17255 0.13 +0.5 3,455,466 - gGDEF BNILDI_17255 0.13 -1.8 3,455,476 + gGDEF BNILDI_17255 0.13 +0.1 3,455,489 + gGDEF BNILDI_17255 0.14 -0.0 3,455,489 + gGDEF BNILDI_17255 0.14 +0.4 3,455,490 - gGDEF BNILDI_17255 0.14 +0.3 3,455,490 - gGDEF BNILDI_17255 0.14 +0.2 3,455,523 - gGDEF BNILDI_17255 0.17 +1.9 3,455,643 - gGDEF BNILDI_17255 0.25 -0.1 3,455,671 + gGDEF BNILDI_17255 0.27 -1.0 3,455,672 - gGDEF BNILDI_17255 0.27 -0.8 3,455,697 + gGDEF BNILDI_17255 0.29 +0.2 3,455,705 - gGDEF BNILDI_17255 0.30 +0.3 3,455,705 - gGDEF BNILDI_17255 0.30 +0.2 3,455,812 + gGDEF BNILDI_17255 0.37 -0.2 3,455,812 + gGDEF BNILDI_17255 0.37 +0.2 3,455,813 - gGDEF BNILDI_17255 0.37 -0.6 3,455,974 + gGDEF BNILDI_17255 0.49 +0.9 3,456,053 - gGDEF BNILDI_17255 0.54 +0.1 3,456,218 + gGDEF BNILDI_17255 0.66 -0.1 3,456,236 + gGDEF BNILDI_17255 0.67 -0.2 3,456,306 - gGDEF BNILDI_17255 0.72 +0.1 3,456,330 - gGDEF BNILDI_17255 0.74 +1.2 3,456,330 - gGDEF BNILDI_17255 0.74 +0.2 3,456,539 + gGDEF BNILDI_17255 0.88 +0.3 3,456,554 - gGDEF BNILDI_17255 0.89 +0.7 3,456,783 + -0.4 3,456,784 - -0.1 3,457,001 + -0.0 3,457,134 + uxaB BNILDI_17260 0.14 +1.5 3,457,135 - uxaB BNILDI_17260 0.14 +1.0 3,457,137 - uxaB BNILDI_17260 0.14 -0.2 3,457,137 - uxaB BNILDI_17260 0.14 -0.0 3,457,224 + uxaB BNILDI_17260 0.20 -0.1 3,457,289 + uxaB BNILDI_17260 0.25 -1.4 3,457,289 + uxaB BNILDI_17260 0.25 +0.2 3,457,311 + uxaB BNILDI_17260 0.26 +1.4
Or see this region's nucleotide sequence