Strain Fitness in Escherichia coli ECRC62 around BNILDI_16940

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmanA and fumB are separated by 198 nucleotidesfumB and fumC are separated by 141 nucleotides BNILDI_16935: manA - mannose-6-phosphate isomerase, at 3,391,589 to 3,392,764 manA BNILDI_16940: fumB - class I fumarate hydratase, at 3,392,963 to 3,394,609 fumB BNILDI_16945: fumC - class II fumarate hydratase, at 3,394,751 to 3,396,154 fumC Position (kb) 3392 3393 3394 3395Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 3392.061 kb on - strand, within manAat 3392.197 kb on + strand, within manAat 3392.386 kb on + strand, within manAat 3392.386 kb on + strand, within manAat 3392.387 kb on - strand, within manAat 3392.388 kb on + strand, within manAat 3392.392 kb on + strand, within manAat 3392.392 kb on + strand, within manAat 3392.393 kb on - strand, within manAat 3392.393 kb on - strand, within manAat 3392.504 kb on + strand, within manAat 3392.733 kb on + strandat 3392.734 kb on - strandat 3392.781 kb on - strandat 3392.804 kb on + strandat 3392.828 kb on - strandat 3392.850 kb on + strandat 3392.853 kb on + strandat 3392.853 kb on + strandat 3392.853 kb on + strandat 3392.854 kb on - strandat 3392.950 kb on - strandat 3393.024 kb on - strandat 3393.027 kb on - strandat 3393.054 kb on + strandat 3393.102 kb on + strandat 3393.142 kb on + strand, within fumBat 3393.215 kb on + strand, within fumBat 3393.215 kb on + strand, within fumBat 3393.215 kb on + strand, within fumBat 3393.215 kb on + strand, within fumBat 3393.216 kb on - strand, within fumBat 3393.216 kb on - strand, within fumBat 3393.216 kb on - strand, within fumBat 3393.308 kb on - strand, within fumBat 3393.369 kb on - strand, within fumBat 3393.371 kb on - strand, within fumBat 3393.377 kb on + strand, within fumBat 3393.377 kb on + strand, within fumBat 3393.377 kb on + strand, within fumBat 3393.377 kb on + strand, within fumBat 3393.378 kb on - strand, within fumBat 3393.378 kb on - strand, within fumBat 3393.378 kb on - strand, within fumBat 3393.421 kb on + strand, within fumBat 3393.421 kb on + strand, within fumBat 3393.421 kb on + strand, within fumBat 3393.425 kb on + strand, within fumBat 3393.425 kb on + strand, within fumBat 3393.426 kb on - strand, within fumBat 3393.426 kb on - strand, within fumBat 3393.426 kb on - strand, within fumBat 3393.428 kb on - strand, within fumBat 3393.473 kb on + strand, within fumBat 3393.494 kb on + strand, within fumBat 3393.542 kb on + strand, within fumBat 3393.542 kb on + strand, within fumBat 3393.542 kb on + strand, within fumBat 3393.543 kb on - strand, within fumBat 3393.543 kb on - strand, within fumBat 3393.543 kb on - strand, within fumBat 3393.543 kb on - strand, within fumBat 3393.570 kb on + strand, within fumBat 3393.647 kb on - strand, within fumBat 3393.688 kb on + strand, within fumBat 3393.689 kb on - strand, within fumBat 3393.717 kb on - strand, within fumBat 3393.839 kb on + strand, within fumBat 3393.982 kb on + strand, within fumBat 3393.995 kb on + strand, within fumBat 3393.995 kb on + strand, within fumBat 3393.995 kb on + strand, within fumBat 3393.996 kb on - strand, within fumBat 3393.996 kb on - strand, within fumBat 3393.996 kb on - strand, within fumBat 3394.200 kb on - strand, within fumBat 3394.205 kb on - strand, within fumBat 3394.301 kb on + strand, within fumBat 3394.302 kb on - strand, within fumBat 3394.302 kb on - strand, within fumBat 3394.384 kb on + strand, within fumBat 3394.385 kb on - strand, within fumBat 3394.434 kb on + strand, within fumBat 3394.450 kb on + strandat 3394.450 kb on + strandat 3394.451 kb on - strandat 3394.637 kb on + strandat 3394.638 kb on - strandat 3394.756 kb on + strandat 3394.808 kb on - strandat 3394.935 kb on + strand, within fumCat 3395.057 kb on - strand, within fumCat 3395.415 kb on + strand, within fumCat 3395.510 kb on - strand, within fumC

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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3,392,061 - manA BNILDI_16935 0.40 -1.5
3,392,197 + manA BNILDI_16935 0.52 -0.4
3,392,386 + manA BNILDI_16935 0.68 +1.0
3,392,386 + manA BNILDI_16935 0.68 -0.5
3,392,387 - manA BNILDI_16935 0.68 +0.4
3,392,388 + manA BNILDI_16935 0.68 -0.1
3,392,392 + manA BNILDI_16935 0.68 +0.1
3,392,392 + manA BNILDI_16935 0.68 +1.5
3,392,393 - manA BNILDI_16935 0.68 +0.3
3,392,393 - manA BNILDI_16935 0.68 +0.3
3,392,504 + manA BNILDI_16935 0.78 -0.1
3,392,733 + -1.4
3,392,734 - -0.8
3,392,781 - -0.6
3,392,804 + -0.0
3,392,828 - +1.4
3,392,850 + -0.2
3,392,853 + +1.8
3,392,853 + +0.7
3,392,853 + -1.4
3,392,854 - +0.1
3,392,950 - -2.7
3,393,024 - -1.1
3,393,027 - -1.4
3,393,054 + -1.2
3,393,102 + -2.0
3,393,142 + fumB BNILDI_16940 0.11 -1.5
3,393,215 + fumB BNILDI_16940 0.15 +0.2
3,393,215 + fumB BNILDI_16940 0.15 -0.2
3,393,215 + fumB BNILDI_16940 0.15 -0.2
3,393,215 + fumB BNILDI_16940 0.15 -1.0
3,393,216 - fumB BNILDI_16940 0.15 -1.4
3,393,216 - fumB BNILDI_16940 0.15 -0.3
3,393,216 - fumB BNILDI_16940 0.15 -0.3
3,393,308 - fumB BNILDI_16940 0.21 -1.1
3,393,369 - fumB BNILDI_16940 0.25 -1.7
3,393,371 - fumB BNILDI_16940 0.25 -0.5
3,393,377 + fumB BNILDI_16940 0.25 -0.2
3,393,377 + fumB BNILDI_16940 0.25 -1.3
3,393,377 + fumB BNILDI_16940 0.25 +1.0
3,393,377 + fumB BNILDI_16940 0.25 -0.2
3,393,378 - fumB BNILDI_16940 0.25 -0.9
3,393,378 - fumB BNILDI_16940 0.25 +0.4
3,393,378 - fumB BNILDI_16940 0.25 -1.2
3,393,421 + fumB BNILDI_16940 0.28 -1.4
3,393,421 + fumB BNILDI_16940 0.28 -1.1
3,393,421 + fumB BNILDI_16940 0.28 -4.0
3,393,425 + fumB BNILDI_16940 0.28 -0.3
3,393,425 + fumB BNILDI_16940 0.28 +0.1
3,393,426 - fumB BNILDI_16940 0.28 -1.4
3,393,426 - fumB BNILDI_16940 0.28 -2.4
3,393,426 - fumB BNILDI_16940 0.28 -3.6
3,393,428 - fumB BNILDI_16940 0.28 -0.6
3,393,473 + fumB BNILDI_16940 0.31 -0.5
3,393,494 + fumB BNILDI_16940 0.32 -0.2
3,393,542 + fumB BNILDI_16940 0.35 -0.7
3,393,542 + fumB BNILDI_16940 0.35 -0.3
3,393,542 + fumB BNILDI_16940 0.35 -2.8
3,393,543 - fumB BNILDI_16940 0.35 -3.5
3,393,543 - fumB BNILDI_16940 0.35 +0.1
3,393,543 - fumB BNILDI_16940 0.35 +0.8
3,393,543 - fumB BNILDI_16940 0.35 -3.1
3,393,570 + fumB BNILDI_16940 0.37 -1.1
3,393,647 - fumB BNILDI_16940 0.42 -0.6
3,393,688 + fumB BNILDI_16940 0.44 -0.1
3,393,689 - fumB BNILDI_16940 0.44 -3.1
3,393,717 - fumB BNILDI_16940 0.46 -0.8
3,393,839 + fumB BNILDI_16940 0.53 -0.1
3,393,982 + fumB BNILDI_16940 0.62 -1.2
3,393,995 + fumB BNILDI_16940 0.63 -3.3
3,393,995 + fumB BNILDI_16940 0.63 +0.4
3,393,995 + fumB BNILDI_16940 0.63 +0.3
3,393,996 - fumB BNILDI_16940 0.63 -2.7
3,393,996 - fumB BNILDI_16940 0.63 +1.1
3,393,996 - fumB BNILDI_16940 0.63 -0.1
3,394,200 - fumB BNILDI_16940 0.75 -2.5
3,394,205 - fumB BNILDI_16940 0.75 -1.5
3,394,301 + fumB BNILDI_16940 0.81 +0.1
3,394,302 - fumB BNILDI_16940 0.81 -0.1
3,394,302 - fumB BNILDI_16940 0.81 -0.5
3,394,384 + fumB BNILDI_16940 0.86 -1.3
3,394,385 - fumB BNILDI_16940 0.86 -0.1
3,394,434 + fumB BNILDI_16940 0.89 -0.6
3,394,450 + -1.4
3,394,450 + -2.0
3,394,451 - -0.8
3,394,637 + +0.9
3,394,638 - -0.3
3,394,756 + +3.2
3,394,808 - +1.4
3,394,935 + fumC BNILDI_16945 0.13 -0.1
3,395,057 - fumC BNILDI_16945 0.22 -2.5
3,395,415 + fumC BNILDI_16945 0.47 +0.4
3,395,510 - fumC BNILDI_16945 0.54 -1.6

Or see this region's nucleotide sequence