Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt manA and fumB are separated by 198 nucleotides fumB and fumC are separated by 141 nucleotides
BNILDI_16935: manA - mannose-6-phosphate isomerase, at 3,391,589 to 3,392,764
manA
BNILDI_16940: fumB - class I fumarate hydratase, at 3,392,963 to 3,394,609
fumB
BNILDI_16945: fumC - class II fumarate hydratase, at 3,394,751 to 3,396,154
fumC
Position (kb)
3392
3393
3394
3395 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3392.061 kb on - strand, within manA at 3392.197 kb on + strand, within manA at 3392.386 kb on + strand, within manA at 3392.386 kb on + strand, within manA at 3392.387 kb on - strand, within manA at 3392.388 kb on + strand, within manA at 3392.392 kb on + strand, within manA at 3392.392 kb on + strand, within manA at 3392.393 kb on - strand, within manA at 3392.393 kb on - strand, within manA at 3392.504 kb on + strand, within manA at 3392.733 kb on + strand at 3392.734 kb on - strand at 3392.781 kb on - strand at 3392.804 kb on + strand at 3392.828 kb on - strand at 3392.850 kb on + strand at 3392.853 kb on + strand at 3392.853 kb on + strand at 3392.853 kb on + strand at 3392.854 kb on - strand at 3392.950 kb on - strand at 3393.024 kb on - strand at 3393.027 kb on - strand at 3393.054 kb on + strand at 3393.102 kb on + strand at 3393.142 kb on + strand, within fumB at 3393.215 kb on + strand, within fumB at 3393.215 kb on + strand, within fumB at 3393.215 kb on + strand, within fumB at 3393.215 kb on + strand, within fumB at 3393.216 kb on - strand, within fumB at 3393.216 kb on - strand, within fumB at 3393.216 kb on - strand, within fumB at 3393.308 kb on - strand, within fumB at 3393.369 kb on - strand, within fumB at 3393.371 kb on - strand, within fumB at 3393.377 kb on + strand, within fumB at 3393.377 kb on + strand, within fumB at 3393.377 kb on + strand, within fumB at 3393.377 kb on + strand, within fumB at 3393.378 kb on - strand, within fumB at 3393.378 kb on - strand, within fumB at 3393.378 kb on - strand, within fumB at 3393.421 kb on + strand, within fumB at 3393.421 kb on + strand, within fumB at 3393.421 kb on + strand, within fumB at 3393.425 kb on + strand, within fumB at 3393.425 kb on + strand, within fumB at 3393.426 kb on - strand, within fumB at 3393.426 kb on - strand, within fumB at 3393.426 kb on - strand, within fumB at 3393.428 kb on - strand, within fumB at 3393.473 kb on + strand, within fumB at 3393.494 kb on + strand, within fumB at 3393.542 kb on + strand, within fumB at 3393.542 kb on + strand, within fumB at 3393.542 kb on + strand, within fumB at 3393.543 kb on - strand, within fumB at 3393.543 kb on - strand, within fumB at 3393.543 kb on - strand, within fumB at 3393.543 kb on - strand, within fumB at 3393.570 kb on + strand, within fumB at 3393.647 kb on - strand, within fumB at 3393.688 kb on + strand, within fumB at 3393.689 kb on - strand, within fumB at 3393.717 kb on - strand, within fumB at 3393.839 kb on + strand, within fumB at 3393.982 kb on + strand, within fumB at 3393.995 kb on + strand, within fumB at 3393.995 kb on + strand, within fumB at 3393.995 kb on + strand, within fumB at 3393.996 kb on - strand, within fumB at 3393.996 kb on - strand, within fumB at 3393.996 kb on - strand, within fumB at 3394.200 kb on - strand, within fumB at 3394.205 kb on - strand, within fumB at 3394.301 kb on + strand, within fumB at 3394.302 kb on - strand, within fumB at 3394.302 kb on - strand, within fumB at 3394.384 kb on + strand, within fumB at 3394.385 kb on - strand, within fumB at 3394.434 kb on + strand, within fumB at 3394.450 kb on + strand at 3394.450 kb on + strand at 3394.451 kb on - strand at 3394.637 kb on + strand at 3394.638 kb on - strand at 3394.756 kb on + strand at 3394.808 kb on - strand at 3394.935 kb on + strand, within fumC at 3395.057 kb on - strand, within fumC at 3395.415 kb on + strand, within fumC at 3395.510 kb on - strand, within fumC
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,392,061 - manA BNILDI_16935 0.40 -1.5 3,392,197 + manA BNILDI_16935 0.52 -0.4 3,392,386 + manA BNILDI_16935 0.68 +1.0 3,392,386 + manA BNILDI_16935 0.68 -0.5 3,392,387 - manA BNILDI_16935 0.68 +0.4 3,392,388 + manA BNILDI_16935 0.68 -0.1 3,392,392 + manA BNILDI_16935 0.68 +0.1 3,392,392 + manA BNILDI_16935 0.68 +1.5 3,392,393 - manA BNILDI_16935 0.68 +0.3 3,392,393 - manA BNILDI_16935 0.68 +0.3 3,392,504 + manA BNILDI_16935 0.78 -0.1 3,392,733 + -1.4 3,392,734 - -0.8 3,392,781 - -0.6 3,392,804 + -0.0 3,392,828 - +1.4 3,392,850 + -0.2 3,392,853 + +1.8 3,392,853 + +0.7 3,392,853 + -1.4 3,392,854 - +0.1 3,392,950 - -2.7 3,393,024 - -1.1 3,393,027 - -1.4 3,393,054 + -1.2 3,393,102 + -2.0 3,393,142 + fumB BNILDI_16940 0.11 -1.5 3,393,215 + fumB BNILDI_16940 0.15 +0.2 3,393,215 + fumB BNILDI_16940 0.15 -0.2 3,393,215 + fumB BNILDI_16940 0.15 -0.2 3,393,215 + fumB BNILDI_16940 0.15 -1.0 3,393,216 - fumB BNILDI_16940 0.15 -1.4 3,393,216 - fumB BNILDI_16940 0.15 -0.3 3,393,216 - fumB BNILDI_16940 0.15 -0.3 3,393,308 - fumB BNILDI_16940 0.21 -1.1 3,393,369 - fumB BNILDI_16940 0.25 -1.7 3,393,371 - fumB BNILDI_16940 0.25 -0.5 3,393,377 + fumB BNILDI_16940 0.25 -0.2 3,393,377 + fumB BNILDI_16940 0.25 -1.3 3,393,377 + fumB BNILDI_16940 0.25 +1.0 3,393,377 + fumB BNILDI_16940 0.25 -0.2 3,393,378 - fumB BNILDI_16940 0.25 -0.9 3,393,378 - fumB BNILDI_16940 0.25 +0.4 3,393,378 - fumB BNILDI_16940 0.25 -1.2 3,393,421 + fumB BNILDI_16940 0.28 -1.4 3,393,421 + fumB BNILDI_16940 0.28 -1.1 3,393,421 + fumB BNILDI_16940 0.28 -4.0 3,393,425 + fumB BNILDI_16940 0.28 -0.3 3,393,425 + fumB BNILDI_16940 0.28 +0.1 3,393,426 - fumB BNILDI_16940 0.28 -1.4 3,393,426 - fumB BNILDI_16940 0.28 -2.4 3,393,426 - fumB BNILDI_16940 0.28 -3.6 3,393,428 - fumB BNILDI_16940 0.28 -0.6 3,393,473 + fumB BNILDI_16940 0.31 -0.5 3,393,494 + fumB BNILDI_16940 0.32 -0.2 3,393,542 + fumB BNILDI_16940 0.35 -0.7 3,393,542 + fumB BNILDI_16940 0.35 -0.3 3,393,542 + fumB BNILDI_16940 0.35 -2.8 3,393,543 - fumB BNILDI_16940 0.35 -3.5 3,393,543 - fumB BNILDI_16940 0.35 +0.1 3,393,543 - fumB BNILDI_16940 0.35 +0.8 3,393,543 - fumB BNILDI_16940 0.35 -3.1 3,393,570 + fumB BNILDI_16940 0.37 -1.1 3,393,647 - fumB BNILDI_16940 0.42 -0.6 3,393,688 + fumB BNILDI_16940 0.44 -0.1 3,393,689 - fumB BNILDI_16940 0.44 -3.1 3,393,717 - fumB BNILDI_16940 0.46 -0.8 3,393,839 + fumB BNILDI_16940 0.53 -0.1 3,393,982 + fumB BNILDI_16940 0.62 -1.2 3,393,995 + fumB BNILDI_16940 0.63 -3.3 3,393,995 + fumB BNILDI_16940 0.63 +0.4 3,393,995 + fumB BNILDI_16940 0.63 +0.3 3,393,996 - fumB BNILDI_16940 0.63 -2.7 3,393,996 - fumB BNILDI_16940 0.63 +1.1 3,393,996 - fumB BNILDI_16940 0.63 -0.1 3,394,200 - fumB BNILDI_16940 0.75 -2.5 3,394,205 - fumB BNILDI_16940 0.75 -1.5 3,394,301 + fumB BNILDI_16940 0.81 +0.1 3,394,302 - fumB BNILDI_16940 0.81 -0.1 3,394,302 - fumB BNILDI_16940 0.81 -0.5 3,394,384 + fumB BNILDI_16940 0.86 -1.3 3,394,385 - fumB BNILDI_16940 0.86 -0.1 3,394,434 + fumB BNILDI_16940 0.89 -0.6 3,394,450 + -1.4 3,394,450 + -2.0 3,394,451 - -0.8 3,394,637 + +0.9 3,394,638 - -0.3 3,394,756 + +3.2 3,394,808 - +1.4 3,394,935 + fumC BNILDI_16945 0.13 -0.1 3,395,057 - fumC BNILDI_16945 0.22 -2.5 3,395,415 + fumC BNILDI_16945 0.47 +0.4 3,395,510 - fumC BNILDI_16945 0.54 -1.6
Or see this region's nucleotide sequence