Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BNILDI_15875 and yoaB are separated by 421 nucleotides yoaB and yoaA are separated by 128 nucleotides yoaA and tsaB are separated by 21 nucleotides tsaB and yeaY are separated by 39 nucleotides
BNILDI_15875: BNILDI_15875 - DUF1889 domain-containing protein, at 3,185,608 to 3,185,907
_15875
BNILDI_15880: yoaB - RutC family protein YoaB, at 3,186,329 to 3,186,676
yoaB
BNILDI_15885: yoaA - putative ATP-dependent DNA helicase YoaA, at 3,186,805 to 3,188,715
yoaA
BNILDI_15890: tsaB - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB, at 3,188,737 to 3,189,468
tsaB
BNILDI_15895: yeaY - Slp family lipoprotein YeaY, at 3,189,508 to 3,190,089
yeaY
Position (kb)
3186
3187
3188
3189 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3185.806 kb on - strand, within BNILDI_15875 at 3185.857 kb on + strand, within BNILDI_15875 at 3185.889 kb on + strand at 3185.894 kb on - strand at 3185.894 kb on - strand at 3185.894 kb on - strand at 3185.896 kb on + strand at 3185.947 kb on - strand at 3185.948 kb on + strand at 3185.951 kb on - strand at 3185.951 kb on - strand at 3186.170 kb on - strand at 3186.173 kb on + strand at 3186.173 kb on + strand at 3186.176 kb on - strand at 3186.178 kb on - strand at 3186.181 kb on + strand at 3186.181 kb on + strand at 3186.182 kb on - strand at 3186.244 kb on - strand at 3186.349 kb on + strand at 3186.349 kb on + strand at 3186.349 kb on + strand at 3186.442 kb on + strand, within yoaB at 3186.604 kb on - strand, within yoaB at 3186.626 kb on + strand, within yoaB at 3186.627 kb on - strand, within yoaB at 3186.630 kb on - strand, within yoaB at 3186.667 kb on + strand at 3186.688 kb on + strand at 3186.689 kb on - strand at 3186.689 kb on - strand at 3186.689 kb on - strand at 3186.690 kb on + strand at 3186.691 kb on - strand at 3186.775 kb on + strand at 3186.776 kb on - strand at 3186.961 kb on + strand at 3186.961 kb on + strand at 3186.967 kb on + strand at 3186.968 kb on - strand at 3187.005 kb on + strand, within yoaA at 3187.006 kb on - strand, within yoaA at 3187.081 kb on + strand, within yoaA at 3187.082 kb on - strand, within yoaA at 3187.120 kb on + strand, within yoaA at 3187.216 kb on - strand, within yoaA at 3187.331 kb on - strand, within yoaA at 3187.332 kb on + strand, within yoaA at 3187.391 kb on + strand, within yoaA at 3188.208 kb on + strand, within yoaA at 3188.218 kb on - strand, within yoaA at 3188.453 kb on + strand, within yoaA at 3188.454 kb on - strand, within yoaA at 3188.713 kb on + strand at 3188.729 kb on - strand at 3189.473 kb on + strand at 3189.473 kb on + strand at 3189.540 kb on - strand at 3189.549 kb on + strand at 3189.549 kb on + strand at 3189.550 kb on - strand at 3189.658 kb on + strand, within yeaY at 3189.659 kb on - strand, within yeaY
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,185,806 - BNILDI_15875 0.66 +0.6 3,185,857 + BNILDI_15875 0.83 -0.1 3,185,889 + -0.2 3,185,894 - +0.7 3,185,894 - +0.3 3,185,894 - +0.1 3,185,896 + -1.4 3,185,947 - +0.8 3,185,948 + -0.2 3,185,951 - -0.8 3,185,951 - -0.1 3,186,170 - -0.7 3,186,173 + +0.1 3,186,173 + +0.2 3,186,176 - -0.1 3,186,178 - -1.1 3,186,181 + +1.0 3,186,181 + -0.7 3,186,182 - -0.5 3,186,244 - +1.5 3,186,349 + -0.3 3,186,349 + -0.1 3,186,349 + +0.4 3,186,442 + yoaB BNILDI_15880 0.32 -0.5 3,186,604 - yoaB BNILDI_15880 0.79 +0.4 3,186,626 + yoaB BNILDI_15880 0.85 +1.3 3,186,627 - yoaB BNILDI_15880 0.86 +0.9 3,186,630 - yoaB BNILDI_15880 0.86 -0.0 3,186,667 + +0.9 3,186,688 + +1.0 3,186,689 - -3.3 3,186,689 - +0.3 3,186,689 - -0.8 3,186,690 + -0.9 3,186,691 - +0.2 3,186,775 + +0.1 3,186,776 - +0.1 3,186,961 + +0.1 3,186,961 + +0.0 3,186,967 + +0.1 3,186,968 - +0.7 3,187,005 + yoaA BNILDI_15885 0.10 +1.6 3,187,006 - yoaA BNILDI_15885 0.11 -0.1 3,187,081 + yoaA BNILDI_15885 0.14 -1.6 3,187,082 - yoaA BNILDI_15885 0.14 -0.8 3,187,120 + yoaA BNILDI_15885 0.16 -2.4 3,187,216 - yoaA BNILDI_15885 0.22 -0.3 3,187,331 - yoaA BNILDI_15885 0.28 -0.5 3,187,332 + yoaA BNILDI_15885 0.28 -0.7 3,187,391 + yoaA BNILDI_15885 0.31 +1.0 3,188,208 + yoaA BNILDI_15885 0.73 -0.8 3,188,218 - yoaA BNILDI_15885 0.74 +0.2 3,188,453 + yoaA BNILDI_15885 0.86 -0.3 3,188,454 - yoaA BNILDI_15885 0.86 +0.3 3,188,713 + -0.2 3,188,729 - -0.5 3,189,473 + -0.0 3,189,473 + +0.8 3,189,540 - -0.4 3,189,549 + +0.2 3,189,549 + +0.0 3,189,550 - +0.7 3,189,658 + yeaY BNILDI_15895 0.26 +0.1 3,189,659 - yeaY BNILDI_15895 0.26 +0.3
Or see this region's nucleotide sequence